Allprep dna rna ffpe mini kit

Manufactured by Qiagen

Sourced in Germany

The AllPrep DNA/RNA FFPE Mini Kit is a laboratory equipment product designed to simultaneously purify both DNA and RNA from formalin-fixed, paraffin-embedded (FFPE) samples. The kit utilizes a streamlined workflow to extract high-quality nucleic acids from a small amount of FFPE tissue.

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Lab products found in correlation

Nanodrop nd 1000, by Thermo Fisher Scientific (1 mentions) Tso500 assay, by Illumina (1 mentions) Tso500 local app software, by Illumina (1 mentions) Nextseq, by Illumina (1 mentions)

Close spelling variants of this product

AllPrep DNA/RNA FFPE Kit (387 citations) AllPrep DNA/RNA FFPE (11 citations) AllPrep DNA/RNA Mini (13 citations) AllPrep DNA FFPE Kit (10 citations) AllPrep FFPE kit (21 citations) Allprep RNA/DNA (2 citations) AllPrep Mini Kit (47 citations) FFPE RNA kit (5 citations) FFPE DNA Kit (17 citations) RNA mini kit (163 citations)

2 protocols using allprep dna rna ffpe mini kit

ERVE4 Expression Profiling in FFPE Tumors

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RNA was extracted from micro-dissected tumor-enriched areas from 4-μm-thick unstained FFPE slides using the AllPrep DNA/RNA FFPE Mini Kit (Qiagen Cat# 80234). RNA concentration was assessed using the NanoDrop ND-1000 (Thermo Fisher Scientific).
Quantitative real-time PCR was performed using reverse transcribed RNA extracted from FFPE tumor tissues to assess levels of pan-ERVE4 and hERV4700-ENV and the reference genes 18S and HPRT (see Supplementary Materials and Methods). A list of forward primers, reverse primers, and probes used for the assay is provided in Supplementary Table S2.

Ficial M., Jegede O.A., Sant’Angelo M., Hou Y., Flaifel A., Pignon J.C., Braun D.A., Wind-Rotolo M., Sticco-Ivins M.A., Catalano P.J., Freeman G.J., Sharpe A.H., Hodi F.S., Motzer R.J., Wu C.J., Atkins M.B., McDermott D.F., Shukla S.A., Choueiri T.K, & Signoretti S. (2020). Expression of T-cell Exhaustion Molecules and Human Endogenous Retroviruses as Predictive Biomarkers for response to Nivolumab in Metastatic Clear Cell Renal Cell Carcinoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 27(5), 1371-1380.

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Comprehensive Cancer Gene Profiling with TSO500

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The TSO500 assay from Illumina (catalog number 20028216) can detect copy number variants, gene fusions, insertions/deletions, single-nucleotide variants, and transcript variants from the RNA of 55 genes and the DNA of 523 genes found across a large range of cancers, including fusions of the NTRK1/2/3 genes. Only the RNA component of the assay was processed: sample input for this assay was 40 ng RNA, as per manufacturer's instructions, isolated using the AllPrep DNA/RNA FFPE Mini Kit from Qiagen (Hilden, Germany; catalog number 80234). Library was prepared and enriched as recommended by the assay protocol. Libraries were multiplexed for sequencing on an Illumina NextSeq (catalog number SY-415-1001) with up to 16 RNA libraries per flowcell. Data were analyzed using the TSO500 Local App software version 2.0.1.4 (Illumina). Samples had to pass the following QC criteria: i) median insert size !80; ii) median CV value of target region coverage across all genes with coverage above 500Â 93; and iii) total on-target reads !9,000,000.

Bormann Chung C., Lee J., Barritault M., Bringuier P.P., Xu Z., Huang W.Y., Beharry A., Castillo J., Christiansen J., Lin J.C, & Sheffield B.S. (2022). Evaluating Targeted Next-Generation Sequencing Assays and Reference Materials for NTRK Fusion Detection. The Journal of molecular diagnostics : JMD, 24(1).

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