Chromatographic separation was achieved on a Waters Acquity® UPLC HSS T3 Column (100 Å, 1.8 μm, 2.1 mm × 100 mm), and products were detected using the Waters SYNAPT G2-Si High Definition Mass Spectrometry with electrospray ionization (operated in the positive ionization mode). Gradient composition and UPLC-MS settings were the same as with the LC1-MS1 method; a TOF-MS/MS mode was applied with a fixed mass on the quadrupole of m/z 865.157, a fixed trap collision energy of 30 eV, and an acquired MS/MS over the range of 100–1450 m/z (scan time 1 s). EntF* retention time was between 3.40-3.50 min. When at least four EntF* daughter ions (m/z ± 0.05) were detected at the expected retention time and at least three of the most abundant isotope parent peaks (m/z ± 0.05) were detected, the sample was considered EntF* positive.
Synapt g2 si high definition mass spectrometry
Manufactured by Waters Corporation
The SYNAPT G2-Si High Definition Mass Spectrometry is a laboratory instrument designed for high-resolution mass spectrometry analysis. It provides accurate mass measurement and enhanced separation capabilities for complex sample analysis.
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2 protocols using synapt g2 si high definition mass spectrometry
1
Chromatographic Separation and Mass Spectrometry Analysis of EntF*
2
Ultra-sensitive EntF* Peptide Detection
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Chromatographic separation was achieved on a Waters Acquity® UPLC HSS T3 Column (100 Å, 1.8 µm, 2.1 mm x 100 mm), with detection using the Waters SYNAPT G2-Si High Definition Mass Spectrometry with electrospray ionization (operated in the positive ionization mode). Gradient composition and UPLC-MS settings were the same as with the LC1-MS1 method; a TOF-MS/MS mode was applied with a fixed mass on the quadrupole of 865.157, a fixed trap collision energy of 30 eV and an acquired MS/MS over the range of 100-1450 m/z (scan time 1 second). EntF* retention was observed between 3.40-3.50 min. When at least four daughter ions (m/z ± 0.05) of EntF* were detected at the expected retention time and at least three of the most abundant isotope parent peaks (m/z ± 0.05) were detected, the sample was considered to contain the EntF* peptide.
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