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2 protocols using rosmarinic

1

UHPLC-DAD-ESI-MS Analysis of Bioactive Compounds

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All solvents used for UHPLC-DAD-ESI-MS, i.e., acetonitrile (99.9%, LC/MS grade) and water (LC/MS grade), were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Petroleum ether (95% GC/MS grade, 40–65 °C) and n-alkanes (C8–C20) were purchased from Carlo Erba Reagents S.A.S. (Barcelona, Spain) and Fluka, Sigma-Aldrich (Burlington, MA, USA), respectively. Methanol (99.8%, HPLC grade) and water (HPLC grade) used for the extraction were purchased from Thermo Fisher Scientific (Waltham, MA, USA). HPLC reference standards rosmarinic (≥99% analytical standard) and carnosic acid (≥90%) were purchased from Extrasynthese (Genay Cedex, France), while luteolin 7-O-glucoside (≥95%) was from Phytolab (Vestenbergsgreuth, Germany). Bovine Serum Albumin (BSA) Fraction V (lyophilized powder >95%) was purchased from Pan Biotech (Aidenbach, Germany), 2-deoxy-d-ribose (>99%) from Alfa Aesar (Kandel, Germany), aminoguanidine bicarbonate salt (>98%) from Sigma-Aldrich (St. Louis, MO, USA), sodium azide (>99%) and trichloroacetic acid (TCA) (>99.5%, pharma grade) from PanReac AppliChem ITW Reagents (Barcelona, Spain). Anhydrous sodium sulphate (>99%) was purchased from Penta Chemicals (Prague, Czech Republic) while disodium hydrogen phosphate (>99%) and sodium dihydrogen phosphate dihydrate (>99%) were purchased from Merck (Kenilworth, NJ, USA).
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2

Antioxidant and Chromatographic Analysis

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For antioxidant tests, 2,2-dipheny-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Ward Hill, MA, USA). For chromatographic analysis, HPLC-grade acetonitrile was from Fisher Scientific (Lisbon, Portugal). The standards, such as fatty acids methyl ester (FAME) reference standard mixture (standard 47885-U), β-carotene and Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were purchased from Sigma (St Louis, MO, USA), as also formic acid. Phenolic compound standards, namely rosmarinic and caffeic acids, were purchased from Extrasynthèse (Genay, France). Sodium alginate was provided from Fluka Chemie (Steinheim, Switzerland). All other chemicals and solvents were of analytical grade and purchased from common sources. Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).
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