Cells were harvested at 48 h after transfection. The cells were washed with PBS and fixed in ethanol at −20°C. The cells were then washed with PBS, rehydrated and resuspended in propidium iodide (PI; 10 µl)-RNase A solution (Sigma-Aldrich; Merck KGaA) at 37°C for 30 min. The stained cells (1×105) were then analyzed for DNA content with a flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). The results were analyzed with FlowJo 7.6.1 software (FlowJo LLC, Ashland, OR, USA).
10 l rnase a solution
Manufactured by Merck Group
The 10 µl)-RNase A solution is a laboratory reagent containing ribonuclease A (RNase A) at a concentration of 10 µl). RNase A is an enzyme that catalyzes the hydrolysis of ribonucleic acid (RNA) molecules, breaking down single-stranded RNA into smaller fragments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 10 l rnase a solution
1
Cell Cycle Analysis by Flow Cytometry
2
Cell Cycle Analysis by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Forty-eight hours post-transfection, cells were harvested. These cells were washed with PBS and fixed in ethanol at −20˚C. The cells were again washed with PBS, rehydrated, and resuspended in propidium iodide (PI; 10 µl)-RNase A solution (Sigma–Aldrich; Merck KGaA) at 37˚C for 30 min. The stained cells (1 × 105) were then analyzed for DNA content using a flow cytometer (BD Biosciences, Franklin Lakes, NJ, U.S.A.). The results were analyzed using FlowJo 7.6.1 software (FlowJo LLC, Ashland, OR, U.S.A.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!