Small interfering RNAs (siRNAs) against Drp1 and Mfn2 siRNA were purchased from Ambion-Applied Biosystems (Austin, TX). Antibodies against Cyclin D1, PCNA (proliferating cell Nuclear Antigen), Caspase3, Caspase9, CytC (Cytochrome C), ATP5A (Mitochondrial ATPase α chain) and SDHA (Succinate Dehydrogenase Subunit A) were obtained from Santa Cruz Biotechnologies (Santa Cruz, CA). Antibodies against Enolase and LDHA (Lactose Dehydrogenase subunit A) were purchased from ProteinTech (Rosemont, IL). Antibodies to Bcl2 were obtained from Abcam (Cambridge, MA), and β-Actin antibodies from Sigma Aldrich (St. Louis MO). Culture plates, chemical reagents and cartridges for the XFe24 Extracellular Flux Analyzer were purchased from SeaHorse Biosciences (Billerica, MA). TMRE and MitoTracker Green dyes, and CyQuant NF Kit was obtained from Invitrogen/ Molecular Probes (Carlsbad, CA). Multiparameter Apoptosis Kit was obtained from Cayman Chemicals (Ann Arbor, MI). Oligonucleotides used as primers in quantitative PCR were synthesized by Integrated DNA Technologies (Coralville, IA). Other chemicals [including 3-Bromopyruvate (3-BP)] were purchased from Sigma Aldrich (St. Louis MO), unless stated otherwise.
Antibodies against cyclin d1
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Antibodies against cyclin D1 are laboratory reagents used in research applications to detect and study the expression of the cyclin D1 protein. Cyclin D1 is a key regulator of the cell cycle and plays a critical role in cell proliferation. These antibodies provide a tool for researchers to investigate the expression and function of cyclin D1 in various biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Xfe24 extracellular flux analyzer, by Agilent Technologies (1 mentions)
3 bromopyruvate 3 bp, by Merck Group (1 mentions)
Multiparameter apoptosis kit, by Cayman Chemical (1 mentions)
Caspase 3, by Santa Cruz Biotechnology (1 mentions)
β actin antibody, by Merck Group (1 mentions)
Nc membrane, by Merck Group (1 mentions)
Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Bsa method, by Keygen Biotech (1 mentions)
Antibody against β actin, by Merck Group (1 mentions)
Mmp 2, by Santa Cruz Biotechnology (1 mentions)
Peroxidase labeled donkey anti rabbit and sheep anti mouse immunoglobulin, by Cytiva (1 mentions)
Enhanced chemiluminescence ecl kit, by Cytiva (1 mentions)
Caspase activity assay kits, by R&D Systems (1 mentions)
Vascular endothelial growth factor (vegf), by Santa Cruz Biotechnology (1 mentions)
C myc, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Icam 1, by Santa Cruz Biotechnology (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Elastase, by Merck Group (1 mentions)
Mercaptoethanol, by Merck Group (1 mentions)
5 protocols using antibodies against cyclin d1
1
Mechanistic Impact of Mitochondrial Dynamics on Cell Signaling
2
Evaluating miR-15b's Protein Targets
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At 48 h after transfection with miR-15b mimics or scramble oligonucleotides, total proteins from control and transfected cells were extracted and the protein concentration was determined by BSA method (keyGEN, China). 30 μg of protein lysates was subjected to SDS-PAGE in 10% acrylamide gel each sample. Then, the electrophoresed proteins were transferred to NC membranes (Millipore Corporation, USA). After that, the membrane was blocked in 5% nonfat milk and incubated with diluted antibodies against Cyclin D1 (1 : 200, Santa Cruz, USA) overnight at 4°C, followed by incubation with HRP-conjugated secondary antibody (1 : 2500, Santa Cruz, USA). After washing with stripping buffer, the membrane was reprobed with GAPDH (1 : 5000, Kangchen, China), using ultra enhanced chemiluminescence western blotting detection reagents. All Western bands were quantified by densitometry and are presented in the form of a bar graph.
3
Anticancer Activity of Vitis coignetiae Pulliat
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Human lung cancer cell lines A549 cells from the American type culture collection (Rockville, MD, USA) were cultured in RPMI 1640 medium (Invitrogen Corp, Carlsbad, CA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS) (GIBCO BRL, Grand Island, NY, USA), 1 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C in a humidified atmosphere of 95% air and 5% CO2. Caspase activity assay kits were obtained from R and D Systems (Minneapolis, MN, USA). Antibodies against cyclin D1, c-Myc, MMP-2, ICAM-1, and VEGF were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against β-actin was from Sigma (Beverly, MA). Peroxidase-labeled donkey anti-rabbit and sheep anti-mouse immunoglobulin, and an enhanced chemiluminescence (ECL) kit were purchased from Amersham (Arlington Heights, IL). All other chemicals not specifically cited here were purchased from Sigma Chemical Co. (St. Louis, MO). The anthocyanins were isolated from Vitis coignetiae Pulliat.
4
Endothelial Cell Proliferation Assay
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M199 medium, streptomycin, penicillin, gelatin, heparin, trypsin, ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), NaOH, elastase, cesium chloride, collagenase, phosphate-buffered saline (PBS), RNase, propidium iodide, glycerol, bromophenol blue, isolectin B4, and mercaptoethanol were from Sigma-Aldrich (St. Louis, MO). Matrigel and endothelial cell growth factor were from BD Biosciences (San Jose, CA). Antibodies against cyclin D1, cyclin E, cyclin A, p21, p27, platelet endothelial cell adhesion molecule-1 (PECAM-1), SGLT2, and β-actin were from Santa Cruz Biotechnology (Santa Cruz, CA), the antibody against SGLT1 was from GeneTex (Irvine, CA), and the antibody against phospho-retinoblastoma protein was from Cell Signaling Technologies (Beverley, MA). [3H]Thymidine (20 Ci/mmol) was from Perkin Elmer (Boston, MA). Canagliflozin, empagliflozin, and dapagliflozin were purchased from Selleck Chemicals (Houston, TX).
5
Immunofluorescence Analysis of Cell Cycle
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Zingerone, Hoechst33342, MTT, 3,3-diaminobenzidine, and antibodies for α-tubulin and β-tubulin were purchased from Sigma Aldrich (St. Louis, MO, USA). Hematoxylin solution was purchased from Merck (Darmstadt, Germany). Antibodies for γ-tubulin and pH3 were purchased from NOVUS (Littleton, CO, USA) and Merck (Darmstadt, Germany), respectively. Antibodies against cyclin D1, cyclin B1, PARP-1, PLK-1, Aurora B, p53, and caspase-3 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Secondary antibodies conjugated to either Alexa-488 or Alexa-594 were purchased from Invitrogen (Eugene, OR, USA).
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