Chelex 100 molecular biology resin

This site uses cookies to ensure you get a better browsing experience. Read our Privacy Policy. OK DNA was extracted using Chelex 100 Molecular Biology Resin (BioRad, Hercules, CA, USA), as previously reported (19 19 Bispo PJ, Hofling-Lima AL, Pignatari AC. Characterization of ocular methicillin-resistant Staphylococcus epidermidis isolates belonging predominantly to clonal complex 2 subcluster II. J Clin Microbiol. 2014;52(5):1412-7. ) . Briefly, fresh isolates grown on 5% sheep blood agar (PROBAC) were cultured in 5 mL of TSB without shaking overnight at 37°C in a 5% CO 2 atmosphere. An aliquot of 1 mL was centrifuged for 5 min at 13,000 rpm (5415R; Eppendorf, Hamburg, Germany), and the cells were washed twice using 1' phosphate buffered saline. The pellets were resuspended in 300 mL of 10% Chelex 100 resin and incubated for 30 min at 95°C. After centrifugation for 5 min at 13,000 rpm, a 1 mL aliquot of the supernatant was collected, diluted 1:10 (v/v), and used for polymerase chain reaction (PCR) amplification.