Trimethylolpropane trimethacrylate

Citrinin (CIT), 1-hydroxy-2-naphthoic acid, 1,4-dihydroxy-2-naphthoic acid, 2,2-azobis isobutyronitrile (AIBN), trimethylolpropane trimethacrylate (TRIM), methacrylic acid (MAA), and PBS tablets were obtained from Sigma (Steinheim, Germany). Barium chloride, N,N-dimethylaminoethyl methacrylate (DAEM), ethylene glycol dimethacrylate (EGDMA), methacrylamide (MAM), salicylic acid, acetic acid, ammonia (25% aq.), sodium hydroxide solution (1M), orthophosphoric acid, and Tween 20 were purchased from Merck (Darmstadt, Germany). LC-grade acetonitrile and methanol were from VWR (Langenfeld, Germany), acetone was from Honeywell (Seelze, Germany). 2-Naphthoic acid was obtained from Acros Organics (Geel, Belgium), 2-oxocyclohexane carboxylic acid was obtained from Fluorochem (Karlsruhe, Germany), and rhodizonic acid dehydrate (RHO) was obtained from abcr (Karlsruhe, Germany). Ochratoxin A (OTA) was provided by HPC Standards (Borsdorf, Germany) and EASI-EXTRACT^® Citrinin columns were provided by R-Biopharm Rhone Ltd. (Glasgow, UK). Cereal products (wheat flour, pasta, rice, whole grain rice crispies, and oat flakes) were purchased from local markets and did not contain detectable levels of CIT. CIT stock and working solution were prepared according to DIN EN 17203 [38 (link)] and stored in acid-washed amber vials at −20 °C.

Styrene (St) (99%), 4-vinylpyridine (4VP) (95%), trimethylolpropane trimethacrylate (TRIM) (90%), divinylbenzene (DVB) (85%), 2,2′-azobis(2-methylpropionitrile) (AIBN) (98%), ibuprofen (IBU) (99%), ketoprofen (KETO) (99%), aspirin (ASP) (99%), and sodium lauryl sulfate (SDS) (99%) were obtained from Sigma-Aldrich. Toluene, methanol, ethanol, tetrahydrofuran (THF), acetone, and methanoic acid were from POCh (Gliwice, Poland). The 1,4-dimethacryloiloxybenzene (14DMB) was obtained in our laboratory according to the earlier described procedure [21 (link)]. PolyStyrene standards for inverse size exclusion chromatography (ISEC) experiments were obtained from Toyo Soda (Tokyo, Japan). THF, acetonitrile, and methanol of HPLC-grade used in chromatography experiments were from Merck Milipore. Monomers: 4VP, TRIM, and Styrene were purified of inhibitor by vacuum distillation and stored in a refrigerator until use. Other reagents were used as received without further purification.

Ammonium hydroxide, azoisobutyronitrile (AIBN), acrylamide, trimethylolpropane trimethacrylate (TRIM) and methanol were sourced from Sigma Aldrich (Gillingham, UK). 3-(Trimethoyxysilyl)propyl methacrylate and amoxicillin were provided by Acros Organics (Geel, Belgium). Phosphate buffered saline (PBS) tablets were purchased from Oxoid (Hampsire, UK). Hydrochloric acid, acetone, and toluene were acquired from Fisher (Loughborough, UK). Hydrogen peroxide (33% solution) was supplied by Alfa Aesar (Heysham, UK). All deionised water (DI) had a resistivity higher than 18 MΩ cm⁻¹.

Bisphenol A (99%), trimethylolpropane trimethacrylate (TRIM), β-CD, MAA, toluene 2,4-diisocyanate (TDI), dimethylacetamide (DMAC), 2,4-dichlorophenol (2,4-DCP), 2,4-dinitrophenol (2,4-DNP) and dibutyltin dilaurate (DBTDL) were bought from Sigma Aldrich USA. Benzoyl peroxide (BPO), methanol (MeOH), iron (II) chloride tetrahydrate (FeCl₂.4H₂O) and iron (III) chloride hexahydrate (FeCl₃.6H₂O) were bought from R&M (Essex, UK). Analytical grade absolute ethanol (denatured, 99.7) and methanol were obtained from HmbG Chemicals (Cologne, Germany), and NH₃ solution (28%) and acetic acid were purchased from Bright Chem SDN. BHD. Nitrogen gas was obtained from Malaysian oxygen (MOx). The stock solution of 1000 mg l⁻¹ BPA, 2,4-DCP and 2,4-DNP was prepared by diluting respective standards in methanol and stored at 4°C. Fresh working standards were prepared daily by diluting the stock solution in deionized water. All reagents and chemicals were of analytical reagent grade and were used as received without further purification.

Ethylene glycol dimethacrylate, methacrylic acid, trimethylolpropane trimethacrylate, 2-hydroxyethyl methacrylate, 5-fluorouracil, 2,2′-azobisisobutyronitrile solution (AIBN; 0.2 M in toluene), and all solvents (HPLC grade) were obtained from Sigma-Aldrich (St. Louis, MO, USA).