Endogro ls medium

Manufactured by Merck Group

Sourced in United States

EndoGRO-LS medium is a cell culture medium designed for the growth and maintenance of human endothelial cells. It provides a defined, serum-free, and low-calcium formulation to support the in vitro culture of endothelial cells.

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EndoGRO (16 citations) EndoGRO medium (10 citations) EndoGRO-LS complete medium (2 citations) EndoGro-LS complete culture medium (2 citations)

4 protocols using endogro ls medium

Cell Culture of Common Cancer Models

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The human pancreatic cancer cell lines AsPC-1 and Panc-1, the human umbilical vein endothelial cells (HUVEC), and the human fibroblast cell line WI-38 were all purchased from the American Type Culture Collection (ATCC). Cells were initially grown and multiple aliquots were cryopreserved. All the cell lines were used within 6 months after resuscitation. AsPC-1 cells were grown in RPMI-1640 medium; Panc-1 and WI-38 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; Sigma), both supplemented with 10% FBS. HUVECs were grown in EndoGRO-LS medium containing endothelial cell growth supplements (Millipore Corporation).

Awasthi N., Zhang C., Schwarz A.M., Hinz S., Schwarz M.A, & Schwarz R.E. (2014). Enhancement of Nab-Paclitaxel Antitumor Activity through Addition of Multitargeting Antiangiogenic Agents in Experimental Pancreatic Cancer. Molecular cancer therapeutics, 13(5), 1032-1043.

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HUVEC Tube Formation Assay

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Low-passage HUVECs (1 × 10⁴ cells/well) were grown in endothelial growth basal medium (EndoGRO-LS medium; Millipore, MA, USA) for 12 h in growth factor-reduced Matrigel (BD Biosciences, NJ, USA)-coated 96-well plates. The tube formation of HUVECs was stimulated by adding 100 ng/mL human recombinant VEGF (Millipore, Burlington, MA, USA) in the presence of vehicle or compound for 12 h. Phenotypic tube formation was monitored every hour from 0 h to 12 h by microscope (Zeiss Imager Z1).

Yang C.C., Chang M.T., Chang C.K, & Shyur L.F. (2021). Phytogalactolipid dLGG Inhibits Mouse Melanoma Brain Metastasis through Regulating Oxylipin Activity and Re-Programming Macrophage Polarity in the Tumor Microenvironment. Cancers, 13(16), 4120.

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Characterization of Gastric Cancer Cell Lines

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The human GAC cell lines AGS, SNU-1, SNU-5, SNU-16, and KATO-III were purchased from the American Type Culture Collection (ATCC, Rockville, MD). Human GAC cell line MKN-45 was purchased from Creative Bioarray (Shirley, NY). The characteristics of these GAC cell lines are presented in Supplementary Table S1 indicating that MKN-45 cells express c-met and E-cadherin oncogenes, while KATO-III cells express c-met and uniquely overexpress FGFR2 oncogenes. The human umbilical vein endothelial cells HUVEC, and the human fibroblast cell line WI-38 were also purchased from ATCC. All these cell lines were tested and authenticated by ATCC. All GAC cells were grown in RPMI 1640 medium (Sigma Chemical Co., St. Louis, MO) containing 10% or 20% FBS and maintained at 37°C in a humidified incubator with 5% CO₂ and 95% air. HUVECs were grown in EndoGRO-LS medium containing endothelial cell growth supplements (Millipore Corp., Billerica, MA). WI-38 cells were grown in DMEM supplemented with 10% FBS.

Awasthi N., Schwarz M.A., Zhang C, & Schwarz R.E. (2018). Augmentation of nab-paclitaxel chemotherapy response by mechanistically diverse antiangiogenic agents in preclinical gastric cancer models. Molecular cancer therapeutics, 17(11), 2353-2364.

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Pancreatic Cancer Cell Lines and Reagents

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The human pancreatic cancer cell lines AsPC-1, BxPC-3, Panc-1, MIA PaCa-2, the human umbilical vein endothelial cells (HUVEC), and the human fibroblast cell line WI-38 were all purchased from the American Type Culture Collection (ATCC, Rockville, MD). Pancreatic cancer associated stromal cells were kindly provided by Dr. Melissa Fishel at the Indiana University Pancreatic Cancer Signature Center, Indianapolis. The murine pancreatic cancer cell line PanO2 was obtained from the National Cancer Institute (Bethesda, MD). Cells were initially grown and multiple aliquots were cryopreserved. All the cell lines were used within 6 months after culture start. AsPC-1 and BxPC-3 cells were grown in RPMI 1640 medium; Panc-1, MIA PaCa-2, PanO2, WI-38 and pancreatic cancer stromal cells were grown in DMEM (Sigma Chemical Co. St. Louis, MO), both media were supplemented with 10% FBS. HUVECs were grown in EndoGRO-LS medium containing endothelial cell growth supplements (Millipore Corp., Billerica, MA). Gemcitabine was purchased from Eli Lilly Corporation (Indianapolis, IN). Nintedanib was purchased from LC Laboratories (Woburn, MA). The cell proliferation reagent WST-1 was purchased from Roche Diagnostic Corporation (Indianapolis, IN).

Awasthi N., Hinz S., Brekken R.A., Schwarz M.A, & Schwarz R.E. (2014). Nintedanib, a triple angiokinase inhibitor, enhances cytotoxic therapy response in pancreatic cancer. Cancer letters, 358(1), 59-66.

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