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Human cxcl12 quantikine elisa kit

Manufactured by R&D Systems
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The Human CXCL12 Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure the concentration of human CXCL12 in cell culture supernates, serum, and plasma. It utilizes a microplate pre-coated with an antibody specific for CXCL12.

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Human CXCL12/SDF-1 alpha Quantikine ELISA Kit Human CXCL12/SDF-1α Quantikine ELISA Kit Human CXCL12 ELISA kit CXCL12 ELISA kits Quantikine ELISA kit Human CXCL12 Quantikine ELISA Quantikine Human Quantikine kits CXCL12

3 protocols using human cxcl12 quantikine elisa kit

1

Quantifying Plasma CXCL12 Levels

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Fasting arterial blood samples for plasma were drawn at cardiac catheterization and stored at −80°C before analysis. CXCL12 levels were measured using the Human CXCL12 Quantikine ELISA kit (R&D systems). Discovery cohort samples were processed at the time of enrollment and replication cohort samples were processed after an average 3.2 years. The inter-assay precision for the CXCL12 assay varies between 8.2% to 13.4% for the different concentrations. In a batch of 20 samples from the discovery cohort that were re-analyzed with the assay kits used for the replication cohort, an insignificant 3.48% (p=0.56) difference in means of CXCL12 level was found. Mean CXCL12 was 1623±606 pg/ml in the discovery cohort compared to 2534±1011 pg/ml in the replication cohort.
Ghasemzadeh N., Hritani A.W., De Staercke C., Eapen D.J., Veledar E., Al Kassem H., Khayata M., Zafari A.M., Sperling L., Hooper C., Vaccarino V., Mavromatis K, & Quyyumi A.A. (2014). Plasma Stromal Cell-Derived Factor 1α/CXCL12 Level Predicts Long-Term Adverse Cardiovascular Outcomes in Patients with Coronary Artery Disease. Atherosclerosis, 238(1), 113-118.
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2

Quantifying CXCL12 Levels in Breast Cancer

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CXCL12 levels were determined in cell-conditioned media of BC cells plated on p100-dishes and incubated in DMEM 1% FCS for 48 h. A human CXCL12 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA) was used according to the manufacturer’s instructions. Protein levels of CXCL12 were calculated relative to the standard curve of the standard samples and were normalized to the amount of the total protein in cell lysates of seeded cells quantified by the Lowry method.
Neves M., Marolda V., Mayor F J.r, & Penela P. (2022). Crosstalk between CXCR4/ACKR3 and EGFR Signaling in Breast Cancer Cells. International Journal of Molecular Sciences, 23(19), 11887.
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3

Quantifying CXCL12 in Bone Marrow Fluid

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Bone marrow extracellular fluid was obtained by crushing bone marrow core biopsies wash crushed using a mortal and pestle in 1 ml of sterile saline. Bone fragments and cells were removed by centrifugation at 500 x g for 3 minutes at 4oC. CXCL12 protein expression was measured using the Human CXCL12 Quantikine ELISA kit from R&D Systems, per manufacturer’s recommendation.
Uy G.L., Hsu Y.M., Schmidt A.P., Stock W., Fletcher T.R., Trinkaus K.M., Westervelt P., DiPersio J.F, & Link D.C. (2015). Targeting Bone Marrow Lymphoid Niches In Acute Lymphoblastic Leukemia. Leukemia research, 39(12), 1437-1442.
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