Mice were euthanized 24 h after intranasal sensitization. Based on the size of the required section area, fresh mouse tissues were soaked in 5% glacial acetic acid aqueous solution for 10–20 min and frozen. The frozen tissue was cut into flake tissue blocks of approximately 2-mm thickness and embedded in paraffin. The sections were then treated, and the pathological changes in the nasal mucosa were investigated using a Hematoxylin and Eosin Staining Kit (Beyotime). The sections were stained with Sirius red to determine eosinophil infiltration in the nasal mucosa (Ansari and Nikpour 2023 (link)). The number of eosinophils in the nasal mucosa was quantified at 400 × magnification.
Hematoxylin and eosin staining kit
Manufactured by Beyotime
Sourced in China
The Hematoxylin and Eosin Staining Kit is a laboratory tool used for staining tissue samples. It contains the necessary reagents to perform the H&E staining technique, which is a widely used method in histology and pathology for the visualization of cellular and subcellular structures.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
3 3 diaminobenzidine (dab), by Vector Laboratories (2 mentions)
Pannoramic midi automatic digital slide scanner, by 3DHISTECH (2 mentions)
Masson s trichrome stain kit, by Solarbio (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)
Bx51 microscope, by Olympus (2 mentions)
Reactive oxygen species assay kit, by Beyotime (2 mentions)
Light microscopy, by Olympus (2 mentions)
Sp pod kit, by Solarbio (2 mentions)
Bmal1, by Abcam (1 mentions)
Dab detection kit streptavidin biotin, by ZSGB-BIO (1 mentions)
Inverted microscope, by Olympus (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Sytox red dead cell stain, by Thermo Fisher Scientific (1 mentions)
Sybr green master mix, by Vazyme (1 mentions)
Fitc annexin 5 apoptosis detection kit, by BD (1 mentions)
Caspase 9 9502, by Cell Signaling Technology (1 mentions)
Solutol hs 15, by MedChemExpress (1 mentions)
3 3 diaminobenzidine (dab), by Solarbio (1 mentions)
111 protocols using hematoxylin and eosin staining kit
1
Quantifying Eosinophil Infiltration in Nasal Mucosa
2
Immunohistochemical Analysis of PAK7 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Slides were deparaffinized, dehydrated through graded alcohols and rinsed with distilled water. Antigen retrieval was carried out by bringing slides to a boil in 1 mM Sodium Citrate Buffer pH 6.0 followed by 20 min at a sub-boiling temperature. Endogenous peroxidase activity was blocked by incubating the slides with 3% H2O2 for 10 min. Slides were washed in PBS, blocked with serum for 30 min and incubated with a rabbit antibody for PAK7 (1:100 dilution, Proteintech, USA) at 4°C overnight in a humidified container. After washing 3 times with PBS, biotin-conjugated anti-rabbit secondary antibody was incubated for 2 h at 37°C at a dilution of 1:300. 3, 3΄-diaminobenzidine (DAB) (Vector laboratories) was used to stain slides for 2 min and coloring reaction stopped using distilled water. Slides were counterstained with Hematoxylin and Eosin Staining Kit (Beyotime Biotechnology Co., Jiangsu, China) to visualize cell nuclei, followed by bluing in running tap water. Finally, the slides were dehydrated, dipped in xylene and mounted. The Pannoramic MIDI automatic digital slide scanner (3DHISTECH Ltd., Budapest, HUNGARY) was used for image processing and quantifications. Quantification of PAK7 staining was done using IHC profiler in ImageJ33 .
3
Temporal Expression of Circadian Genes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The first molars were dissected from the E14.5 d, E16.5 d, E18.5 d, P.N.4 d, P.N.7 d, P.N.10 d, and P.N.15 d rats (Figures 1 , 2 ) and the E16.5d p75NTRExIII−/− and p75NTRExIII+/+ mice (Figure 4 ), fixed in 4% paraformaldehyde, decalcified with 10% EDTA, and embedded in paraffin. The 6-μm sections of tissue specimens were obtained for H.E. and immunohistochemistry staining. The primary antibodies were used in this study are as follows: rabbit anti-rat p75NTR (1:1,500; Abcam, Cambridge, MA, United States, ab245134, monoclonal), rabbit anti-rat BMAL1 (1:1,000; Abcam, Cambridge, MA, United States, ab230822, monoclonal), rabbit anti-rat CLOCK (1:1,000; Abcam, Cambridge, MA, United States, ab3517, polyclonal), rabbit anti-rat PER1 (1:500; Bioss, Beijing, China,bs-2350R, polyclonal), rabbit anti-rat CRY1 (1:500; Bioss, Beijing, China bs-11441R, polyclonal), rabbit anti-rat ALP (1:500; Bioss, Beijing, China, bs-2928R, polyclonal), rabbit anti-rat COL1 (1:500; Bioss, Beijing, China, bs-0578R, polyclonal). These specimens were treated with the DAB Detection Kit Streptavidin-Biotin (ZSGB-BIO, Beijing, China) and Hematoxylin and Eosin Staining Kit (Beyotime, Shanghai, China) according to the manufacturer’s protocols, followed by visualization under phase-contrast microscopy.
4
Photodynamic Therapy Evaluation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following treatment with TMPyP4 for 4 h, the cells were incubated for an additional 24 h prior to morphological analysis of the cells observed under an inverted microscope (Olympus, Tokyo, Japan). The laser energy density was set at 6 J/cm2 and the cells were stained using a hematoxylin and eosin staining kit (Beyotime Institute of Biotechnology).
5
Histological Analysis of Gouty Arthritis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Joints were collected from mice with gouty arthritis, fixed with 10% formalin, and embedded in paraffin. The joints were then cut into 4-μm thick sections and stained with a Hematoxylin and Eosin Staining Kit (Beyotime Biotechnology, China) for histological examination. Images were acquired using a microscope (Olympus, Japan).
6
Organ Damage Assessment after MI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On day 28 after MI, whole blood was collected, and serum Cr, BUN, ALT, and AST levels were measured using a biochemical analyzer (Chemray240, China). Major organs (heart, liver, lung, kidney, spleen) were harvested for H&E staining (Hematoxylin and Eosin Staining Kit, Beyotime, Shanghai, China) and TUNEL staining according to instructions.
7
Anticancer Effects of MY11 Compound
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MY11 (purity 95%) was synthesized and provided by pharmaceutical chemistry laboratory, East China University of Science and Technology, China [23 (link)]. RPMI 1640 and DMEM was purchased from Hyclone (Logan, UT, USA). MEM, Penicillin/Streptomycin and Fetal Bovine Serum (FBS) were obtained from Gibco (Invitrogen Corporation, New York, USA). Antibodies for Cyclin D1 (A19038), PUMA (A3752), Bcl-2 (A19693), Bax (A19684), NF-κB p65 (A19653), Phospho-NF-κB p65-S536 (AP0124), β-Actin (AC004) and secondary antibodies were purchased from ABclonal (Wuhan, China). Caspase-9 (#9502) and p21 (#2947) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). FITC Annexin V Apoptosis Detection Kit were purchased from BD Biosciences Company. SYTOX Red dead cell stain was purchased from Invitrogen. SYBR green master mix was purchased from Vazyme. Solutol HS-15 was obtained from MedChemExpress. Hematoxylin and Eosin Staining Kit were purchased from Beyotime.
8
Histological Analysis of Organ Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue samples from the intestine, heart, liver, lungs and kidneys were fastened with 10% formalin, embedded with paraffin and then cut into sections (5 μm). The sections were subject to hematoxylin-eosin (H&E) staining using a Hematoxylin and Eosin Staining Kit (Beyotime, Shanghai, China) in accordance with the manufacturer's protocol. Finally, the images were captured using a microscope.
9
Immunohistochemical Analysis of Prostate Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human or rat prostate tissue was routinely paraffin embedded after fixation. The samples were cut into 5 μm thick slices, deparaffinized in xylene and dehydrated with graded alcohol. Antigen extraction was carried out in a 10 mM sodium citrate buffer at pH 6.0, heated to 96 °C for 30 min. Endogenous peroxidase activity was quenched by 3% hydrogen peroxide for 15 min. After rinsing and sealing the slides, incubate overnight in the corresponding primary antibody (listed in Additional file 1 : Table S1) at 4 ℃. Biotin-conjugated anti-rabbit secondary antibody was incubated for 2 h at 37 °C. The slides were stained with DAB (Solarbio, CHN) and counterstained with Hematoxylin and Eosin Staining Kit (Beyotime, CHN). The results were observed and captured by fluorescence microscope.
10
Histopathological Tissue Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Histopathology of the tumors and organs was assessed by a hematoxylin and eosin staining kit (Beyotime), according to the manufacturer’s instructions. The results were evaluated by a pathologist.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!