Magnolol concentration in MAG-SMOF samples was determined using the UV-Vis spectrophotometry method at room temperature on a Lambda 20 UV/Vis Spectrophotometer (PerkinElmer, Shelton, CT, USA). To collect the UV–Vis absorption spectra, 100 µL of MAG-SMOF was dissolved in 1 mL of dichloromethane, and the sample was filled up to 10 mL with methanol. Each sample was transferred to 1.0 cm quartz cells, and the measurements were conducted with respect to the intensity of light passing through a blank sample consisting of 100 µL of SMOFlipid (without the addition of magnolol), 1 mL of dichloromethane and filled up to 10 mL with methanol. The concentration of magnolol was calculated based on calibration curve performer in the range of 0.004 to 0.044 mg/mL with absorption determined at 291.2 nm. The calibration curve was performed by the dilution of the appropriate volume of standard stock solutions of magnolol in methanol (1 mg/mL) in a 10 mL of standard diluent obtained by the binary mixture of dichloromethane and methanol (1:9) and the addition of 100 µL of SMOFlipid.
Lambda 20 uv vis spectrophotometer
Manufactured by PerkinElmer
Sourced in United States
The Lambda 20 UV VIS Spectrophotometer is a dual-beam instrument designed for ultraviolet and visible spectroscopy. It measures the absorption or transmission of light through a sample across a specified wavelength range. The instrument features a monochromator, photodetectors, and a data processing system to analyze the spectral data.
Automatically generated - may contain errors
Lab products found in correlation
Frontier ft ir fir spectrometer, by PerkinElmer (2 mentions)
Pcb 1500 water peltier system, by PerkinElmer (1 mentions)
Ptp 6 6 peltier system, by PerkinElmer (1 mentions)
Lambda 950 uv vis nir spectrophotometer, by PerkinElmer (1 mentions)
Tripure isolation reagent, by Roche (1 mentions)
Pyris 1 tga apparatus, by PerkinElmer (1 mentions)
Agilent 5977a, by Agilent Technologies (1 mentions)
7890b gc system, by Agilent Technologies (1 mentions)
Kaleidagraph, by Synergy Software (1 mentions)
Tga q5000, by TA Instruments (1 mentions)
Kbr pellets, by Thermo Fisher Scientific (1 mentions)
25 protocols using lambda 20 uv vis spectrophotometer
1
Magnolol Quantification in MAG-SMOF
2
Antioxidant Activity of Propolis Extract
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The raw propolis samples were macerated and continuously homogenized for 24 h in 70% ethanol solution (1:100 w/v), and then evaporated to dryness. A reaction mixture containing 2,2-diphenyl-1-picrylhydrazyl (DPPH) 0.1 mM ethanoic solution and 0.6 mg/mL propolis solution was prepared. The absorbance was measured in a quartz cuvette (1 cm3) at λ = 515 nm with a Lambda 20 UV VIS Spectrophotometer (Perkin Elmer UV/VIS, Washington, DC, USA). Absorbance (A) was measured at the initiation of the reaction, then after 10 and 20 min. The antioxidant activity was calculated using the formula [65 ,66 (link)]: %RSA = (ADPPH − Asample)/ADPPH × 100.
3
Optical and Electrochemical Characterization of Polymers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 2
Film UV-Vis absorption spectra of polymers from Example 2 were acquired on a Perkin Elmer Lambda 20 UV/VIS Spectrophotometer. All film samples were spin-cast on ITO/ZnO substrates. Solution UV-Vis absorption spectra at elevated temperatures were collected on a Perkin Elmer Lambda 950 UV/VIS/NIR Spectrophotometer. The temperature of the cuvette was controlled with a Perkin Elmer PTP 6+6 Peltier System, which is supplied by a Perkin Elmer PCB 1500 Water Peltier System. Before each measurement, the system was held for at least 10 min at the target temperature to reach thermal equilibrium. A cuvette with a stopper (Sigma Z600628) was used to avoid volatilization during the measurement. The onset of the absorption is used to estimate the polymer bandgap.
Cyclic voltammetry was carried out on a CHI760E electrochemical workstation with three electrodes configuration, using Ag/AgCl as the reference electrode, a Pt plate as the counter electrode, and a glassy carbon as the working electrode. Polymers were drop-cast onto the electrode from DCB solutions to form thin films. 0.1 mol L−1 tetrabutylammonium hexafluorophosphate in anhydrous acetonitrile was used as the supporting electrolyte. Potentials were referenced to the ferrocenium/ferrocene couple by using ferrocene as external standards in acetonitrile solutions. The scan rate is 0.1 V s−1.
4
Extraction of Total RNA from Rat Brain Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from the brain tissue specimens of rats using a TriPure isolation reagent (Roche Diagnostics, Mannheim, Germany), according to the manufacturer’s instructions. Briefly, fresh or frozen tissues (~100 mg) were homogenized in 1 ml TriPure reagent and submitted to centrifugation at 21,578 × g for 10 min at 4°C. Following the addition of 0.2 ml chloroform to the supernatants, the mixture was incubated at room temperature for 20 min with occasional stirring. The samples were then submitted to centrifugation again, as aforementioned. The resulting supernatants were mixed with 0.5 ml isopropanol, incubated for 5–10 min and centrifuged as aforementioned. The pellet containing total RNA was washed with 1 ml ethanol (75%) and centrifuged at 8,429 × g for 5 min at 4°C. The RNA pellet was air dried, dissolved in 3 ml diethylpyrocarbonate-treated water and the optical density (OD) values were measured at 260 and 280 nm on a Lambda 20 UV/Vis spectrophotometer (Perkin-Elmer, Norwalk, CT, USA). The RNA concentration was calculated as follows: RNA concentration (μg/ml) = OD260 × dilution factor × 40/1,000. RNA purity was assessed by the OD260/OD280 ratio.
5
Nanodisc Formation from DMPC/DMTAP Lipids
6
Quantifying Propolis Phenolics via Folin-Ciocalteu
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For this determination, the Folin–Ciocalteu method was used [61 (link),62 (link)]. Dried raw propolis was grounded in a mixer to a fine powder, dissolved and homogenized in ethanol and filtered. An equivalent quantity of Folin–Ciocalteu reagent was added. The absorbance was measured against a blank (distilled water) at 765 nm with a Lambda 20 UV VIS Spectrophotometer (Perkin Elmer UV/VIS, Washington D.C., USA). The total phenolic concentrations were compared to a standard curve of gallic acid.
7
Determination of HMF in Honey
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the determination of HMF in honey, 10 g of honey was dissolved in approximately 25 mL of distillate water and transferred to a 50 mL volumetric flask; 2 mL of honey solution and 5 mL of p-toluidine were placed in two test tubes. In one tube 1 mL of distilled water was added (reference solution) and in the other 1 mL of barbituric acid solution 0.5% (sample solution). The absorbance was read in 1 cm cuvettes at 550 nm with a Lambda 20 UV VIS Spectrophotometer (Perkin Elmer, Waltham, MA, USA). The HMF content was determined by the external standard method (p 99%, Sigma-Aldrich, Milan, Italy) and by using the proposed formula for the method [53 ].
8
Dbp5 ATPase Kinetics and Stimulation
9
Quantifying Brain Lipid Peroxidation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The quantitative measurement of lipid peroxidation in the whole brain was measured according to the method of Wills (1966 (link)). The amount of malondialdehyde (MDA) concentration formed was measured by the reaction with thiobarbituric acid at 532 nm using Perkin Elmer lambda 20 UV-vis spectrophotometer (Norwalk, CT, USA). The results were expressed as nano moles of MDA per milligram protein using the molar extinction coefficient of chromophore (1.56 × 10 M/cm) and represented as percentage of naive.
10
Characterization of Novel Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the reagents were purchased from Sigma Aldrich and used without any further purification. Infrared spectra (FTIR) were obtained using KBr pellets or NaCl windows on a Perkin-Elmer Frontier FT-IR/FIR spectrometer. Electronic spectra were recorded in methanol on a Perkin Elmer Lambda 20 UV–Vis spectrophotometer. Thermogravimetric analysis (TGA) was carried out with a Perkin-Elmer Pyris 1 TGA apparatus under an N2 atmosphere at a flow rate of 10 mL/min over a temperature range of 25 to 800 °C at a heating rate of 5 °C/min and analyzed using the Pyris version 11.1.1.0492 software package. Electron paramagnetic resonance spectra (EPR) of polycrystalline samples at 298 K and solution at 77 K were recorded in quartz tubes with a Joel JES-TE300 spectrometer equipped with a cylindrical cavity (TE011 mode) operating at X band frequency (9.4 GHz) at 100 KHz field modulation. Gas chromatograms were recorded on an Agilent 7890B-GC System equipped with a mass selective detector, the Agilent 5977A.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!