All determinations were performed in triplicate. Means and standard deviations for all quality classes are summarized in Supporting Information: Tables S1-S4. Pearson and Spearman correlation coefficients were calculated in R (Version 4.1.1) using the Hmisc package. Significant correlations were present at a significance level of p < .05. Correlations were classified as very weak (.41 ≤ r < .54), weak (.54 ≤ r < .67), medium (.67 ≤ r < .78), and strong (r ≥ .78). A principal component analysis (PCA) was performed to evaluate multivariate correlations between all parameters using Origin 2020 (OriginLab Corporation). One-way analysis of variance to identify significant differences between samples sorted by quality class was performed in Origin 2020 (OriginLab Corporation). Differences were identified with Tukey's test at a significance level of p < .05. The PLS-regressions were calculated with The Unscrambler X (Camo Analytics) using the nonlinear iterative partial least squares (NIPALS) algorithm. All PLS-regression models were cross validated.
Origin 2020
Manufactured by OriginLab
Sourced in United States
Origin 2020 is a data analysis and graphing software package. It provides a range of tools for data visualization, analysis, and report generation. The software supports a variety of data formats and can be used for a wide range of applications, including scientific research, engineering, and business analytics.
Automatically generated - may contain errors
Lab products found in correlation
Prism 8, by GraphPad (4 mentions)
Prism 9, by GraphPad (3 mentions)
Amos 24, by IBM (2 mentions)
Spss 19, by IBM (2 mentions)
Spss 26 statistical software, by IBM (2 mentions)
Ix51 inverted microscope, by Olympus (2 mentions)
Instat 3, by GraphPad (2 mentions)
Ftir spectrometer, by PerkinElmer (1 mentions)
Till polychrome 5 digital imaging system, by Toptica (1 mentions)
Spss statistics 26 program, by IBM (1 mentions)
Parstat 2273, by Ametek (1 mentions)
Spss statistical software, by IBM (1 mentions)
Alpha ftir spectrometer, by Bruker (1 mentions)
Avance 700 mhz, by Bruker (1 mentions)
Topspin, by Bruker (1 mentions)
Matlab r2021a, by MathWorks (1 mentions)
Typhoon trio imager, by GE Healthcare (1 mentions)
Spss 25, by IBM (1 mentions)
Spss statistics 22, by IBM (1 mentions)
Matlab r2023a, by MathWorks (1 mentions)
117 protocols using origin 2020
1
Multivariate Analysis of Biomaterial Quality
2
Structural Analysis of Biomaterial Scaffolds
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FTIR spectra were recorded on a PerkinElmer FTIR spectrometer in the spectral range 400–4000 cm−1 with a resolution of 1 cm−1 in transmission mode. The FTIR measurements were repeated three times at random locations for each scaffold type to minimize the possibility of error. In order to minimize the interference of water peaks and peptides in the amide I region, entire samples were dried by vacuum dryer. Data processing was performed using Origin 2020 software (OriginLab Corporation, Northampton, MA, USA). All the measured spectra are background-corrected and normalized. A peak analyzer was used to perform non-linear fitting of the peaks in the spectral data. Baseline corrections were performed using a second derivative (zeroes) method for finding anchor points and detecting the baseline. Hidden peaks were also detected in the spectral range 1500–1700 cm−1 by a second derivative method followed by smoothing with the ten-point Savitsky–Golay function with polynomial order of 2. The deconvoluted spectra were fitted with the Gaussian function. The positions and the number of the components (used as an input file for the curve-fitting function) were obtained from both the second derivative and the deconvoluted spectra. The quality of the fitting was estimated by standard deviation.
3
Statistical Analysis of Experimental Data
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Origin 2020 software (OriginLab Corporation, Northampton, MA, USA) was used to compare the statistical significance between the groups. One-way analysis of variance and t-test were conducted to assess significance, and Tukey’s post hoc test was conducted for post hoc analysis. p<0.05 was considered statistically significant. All results were obtained from three or more independent experiments and expressed as mean±SD.
4
Statistical Analysis of Experimental Data
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Statistical analysis was conducted using Origin 2020 software (OriginLab Corporation, MA, USA). Data are presented as the mean ± standard deviation of observations obtained from more than three independent experiments. Statistical differences were analyzed using one-way ANOVA followed by Tukey’s post hoc test and T-test. Values of p < 0.05 were considered statistically significant.
5
Calcium Imaging of OLE Cells
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OLE cells were grown on 25 mm circular coverslips and incubated with the fluorescent Ca2+ indicator Fura-2AM (2 μM) for 1 h at RT in normal extracellular solution (NES) (137 NaCl mM, 5 mM KCl, 1 mM MgCl2, 2 mM CaCl2, and 10 mM HEPES and pH adjusted to 7.4 by NaOH). Coverslips were washed and incubated with NES for 30 min at RT in the dark. The coverslips were placed in a stainless-steel holder (bath volume ∼0.8 mL) and imaged using a Leica DMI300 B inverted microscope coupled to a TILL Polychrome V digital imaging system (Toptica Photonics, Farmington, NY, USA). Cells were treated with flow-through of 1 µM P4 for 100–400 seconds, 10 nM for 500–700 s, and 80 mM KCl at 800 s. In parallel, a second coverslip of cells was pretreated with 10 nM nicardipine for 30 min followed by the same treatment paradigm described previously. Results present the ratio (R/R0) of fluorescence intensities at the excitation wavelengths of 340 and 380 nm. Ca2+ imaging data were analyzed using Origin 2020 Software (Origin Lab, Northampton, MA, USA), and data for all figures are expressed as mean ± s.e.m. Statistical significance was calculated using one-way ANOVA followed by Student’s t-test and ** represents statistical significance (**P < 0.01).
6
Lettuce Growth Responses to Light
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This experiment was designed as a single factor experiment. A one-way ANOVA was performed for each treatment. When the ANOVA result was significant, Duncan's multiple range test at p < 0.05 was used for mean separation. The linear relationships between plant stem length and G/B ratio were determined by GraphPad Prism 8 (GraphPad Software Inc., San Diego, CA, USA), using simple linear regression. The fitting light intensity response curves of Pn and gs with non-linear regression were performed by the GraphPad Prism 8 (GraphPad Software Inc., San Diego, CA, USA), using [Agonist] vs. response–variable slope (four parameters) equation. The data of chlorophyll concentration in different layers of lettuce plants were performed by a box-plot and drawn with the Origin 2020 software (OriginLab Inc., Northampton, MA, USA). The desperation of data is proportional to the height of the box. The lines and dots in the box represent the median and the average values, respectively. The highest and the lowest point of the vertical line represent the maximum and minimum values. All statistical analyses were performed by the IBM SPSS Statistics 26 program (SPSS Inc., Chicago, IL, USA). This experiment was repeated three times.
7
Comprehensive Statistical Analysis Protocol
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Statistical analyses were performed for each experiment, and appropriate tests were chosen based on the type of data and research question. Data normalization was performed using Excel 2016 (Microsoft, Redmond, WA, USA). For physical characterizations, the data was analyzed and expressed using Origin 2020 software (OriginLab Corporation, Northampton, MA, USA) as mean ± standard deviation to evaluate the relationship between variables. Data analysis and graphing were conducted for in vitro and in vivo experiments utilizing Excel and Prism 8 (GraphPad Software, San Diego, CA, USA). One-way analysis of variance (ANOVA) or t-test was performed to determine the degree of statistical significance between groups. Significance levels were set at a p-value < 0.05 for all analyses.
8
Comprehensive Data Analysis Protocol
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Figures were produced using Microsoft Excel 2016. Variance analysis was performed using the SPSS software (version 15.0). All data were analyzed by two-way ANOVA, the normality of residuals and the homogeneity of variance should be tested using two-way ANOVA. The significance was determined at the 5% significance level. All results are presented as means plus or minus the standard error (SE). Graphical representations and illustrations were created using Origin 2020 software(Origin Lab, USA).
9
Electrochemical Analysis of Zinc-Magnesium-Copper Alloys
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Electrochemical analysis was performed in Hanks’ modified solution at 37 ± 1 °C and pH 7.4 using a PARSTAT 2273 potentiostat (Princeton Applied Research, USA). Saturated calomel electrode (SCE) and platinum electrode were used as the reference electrode and counter electrode, respectively. After 30 min of stabilization of the open circuit potential (OCP), polarization curves were obtained by scanning from −1.4 to −0.6 V potential against the OCP at a scan rate of 0.16 mV/s, according to ASTM G5-14 [40 (link)]. The corrosion current density (icorr) and the corrosion potential (Ecorr) were determined by extrapolating the cathodic Tafel line with Origin 2020 software (OriginLab, USA). The corrosion rate (CR) (μm/year) was calculated from the corrosion current density using Eq. (1) [42 (link)]: where icorr is the corrosion current density (μA/cm2), EW is the equivalent metal weight and ρ is the metal density. According to ASTM G102-89 [42 (link)], the EW and ρ used for calculation were 32.68, 32.42, 32.15, 33.70, 34.41, and 7.14, 7.11, 7.09, 7.19, 7.23 g/cm3 for Zn, Zn-0.5Mg, Zn-1Mg, Zn-3Cu, and Zn-5Cu, respectively.
10
Statistical Analysis of PM Varieties
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SPSS statistical software (version 21.0, SPSS Inc., Chicago, IL, USA) was used to analyze the experimental data. The data are expressed as the mean ± standard deviation of three determinations, and the significant differences among the PM varieties processed by different methods were determined followed by one-way analysis of variance (ANOVA) with Duncan’s test (p < 0.05). Origin 2020 software (OriginLab Corp., Northampton, MA, USA) were used for data processing and chart drawing.
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