Trizma buffer

Manufactured by Merck Group

Sourced in United States

Trizma buffer is a commonly used buffering agent in various laboratory applications. It is a zwitterionic organic compound that maintains a stable pH range, allowing for consistent and reliable experimental conditions. The core function of Trizma buffer is to regulate the pH of solutions, ensuring optimal and reproducible results in a variety of laboratory settings.

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Lab products found in correlation

13 protocols using trizma buffer

Protease Cleaving Mesh Matrix pH Adjustment

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Example 3

The pH of the sample lysate/proteolytic product flowing from the protease cleaving mesh matrix is about 7.5 to 9.0. This pH was adjusted to a pH compatible with the pH of the FAOX enzyme in the reaction zone.

A 200 mM Trizma Buffer solution was prepared with Trizma Buffer—Sigma t4661—by adding about 12 grams of the Trizma Buffer to 400 mL of distilled water and stirring until dissolved. The pH was adjusted to 7.5 at a total solution volume of 500 mL. About 0.5 grams of HEC was added to the solution and stirred during which time 0.05 grams CaboSil 720, 0.10 grams of Avicel Cl-611F, and 0.125 grams of Vinac XX-210 were added. The mixture was homogenized, and the pH adjusted to 8.0.

Polymer dispense: A Biodot PixSys 3200 dispenser was used to dispense 2 uL of the mixture in the region downstream of the second chemical reaction zone, but before the electrical reaction zones. The test sensors were then dried at 45° C. for about 15 minutes.

US11199551B1. Test sensors, systems, and analysis techniques for measuring glycated hemoglobin in undiluted blood samples (2021-12-14). None [US]. Inventors: Jim Connolly [US].

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Bottlenose Dolphin Urine LH EIA

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A double antibody LH EIA has been previously described and validated for bottlenose dolphin urine (Robeck et al. 2005 (link)). Briefly, microtiter plates pre-treated with goat anti-mouse IgG (X12-1EA, Arbor Assays, Ann Arbor, MI) were blocked with 0.02 M Trizma buffer (Sigma–Aldrich) and incubated at room temperature overnight or up to 3 weeks. Plates were emptied, washed (0.02% Tween solution, Sigma–Aldrich) and neat urine (0.05–0.001 mL) and standards (bovine LH, NIH-LH-B10, AFP-5551B, National Institute of Health, Bethesda, MD, USA) were added to wells along with 0.1 mL of antibody (monoclonal anti-bovine LH, LH 518-B7, 1:600,000, J. Roser, UC Davis, CA, USA) and incubated at room temperature overnight. Biotinylated LH (1:1,500,000) was then added, and after another incubation (4 h, room temperature), the plates were washed and streptavidin peroxidase solution (1 µL in 24 mL assay buffer, Roche Diagnostics) was added. Following 40 min incubation, TMB substrate was added prior to a final incubation (45–60 min). A stopping solution was then added (0.6 M H₂SO₄) and the optical density was measured at 450 nm with a 655 nm reference filter. Intra-assay variation was <10% and inter-assay variation was 13.3 and 12% at 30 and 60% binding (n = 35), respectively.

Montano G., Clough P., Schmitt T., Davis M., Steinman K., O’Brien J, & Robeck T. (2022). Follicular and hormonal changes after estrous synchronization in bottlenose dolphins. Reproduction & Fertility, 3(3), 238-254.

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Potentiometric Sensor for Thallium Detection

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All chemicals and reagents used were of analytical grade and were used as received and prepared with de-ionized water. For membrane preparation, high molecular weight poly (vinyl chloride) (PVC), O-nitrophenyloctyl ether (o-NPOE), Tetrahydrofurane (THF), and sodium tetraphenylborate (NaTPB) were used as received from Fluka or Merck. Multiwall carbon nanotubes (MWCNTs) were purchased from (EPRI, cairo, Egypt). Sodium sulphide (Na₂S), sodium iodide (NaI), sodium bromide (NaBr), and Trizma^® buffer were purchased from Sigma Aldrich. A 0.1 M stock solution of TlNO₃ was freshly prepared.

Hassan S.S., Abdelbasir S.M., Fathy M.A., Amr A.E., Al-Omar M.A, & Kamel A.H. (2019). Gold Plate Electrodes Functionalized by Multiwall Carbon Nanotube Film for Potentiometric Thallium(I) Detection. Nanomaterials, 9(8), 1160.

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Chromium(VI) Quantification Using Rhodamine-B Complex

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All chemicals and reagents used in this work were of analytical reagent grade unless otherwise stated and doubly distilled water was used throughout. Whatman qualitative filter paper (Grade 5, COTRAN833 (KC63) was used. High molecular weight poly (vinyl chloride) (PVC) and o-nitrophenyloctyl ether (o-NPOE) were used as received from Fluka (Ronkonoma, NY, USA). Conductive carbon paint was purchased from (Merck, Darmstadt, Germany). Hydrogen peroxide (30%). Tetrahydrofuran (THF), Rhodamine-B (RB), potassium chromate, and Trizma^® buffer were purchased from Sigma Chem. Co (St. Louis, MO, USA).
A 1.0 × 10⁻¹ mol L⁻¹ stock solution of CrO₄²⁻ was freshly prepared in de-ionized water. Working chromium (VI) solutions (1.0 × 10⁻²–1.0 × 10⁻⁶ mol L⁻¹) were prepared by accurate dilutions, and stored in brown bottles. Rhodamin-B /chromate ion pair complex was prepared by mixing equal volumes of 1.0 × 10⁻² mol L⁻¹ of Rhodamine-B and potassium chromate. The red precipitated complex was collected by filtration, washed with distilled water, dried in air, and ground to fine powders.

Hassan S.S., Kamel A.H., Amr A.E., Abdelwahab Fathy M, & Al-Omar M.A. (2020). Paper Strip and Ceramic Potentiometric Platforms Modified with Nano-Sized Polyaniline (PANi) for Static and Hydrodynamic Monitoring of Chromium in Industrial Samples. Molecules, 25(3), 629.

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Potentiometric Sensing of DMA+ Ions

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All chemicals were of the highest purity and used as received. Dimethylamine (DMA), sodium tetraphenyl borate (NaTPB), o-nitrophenyloctyl ether (o-NPOE), high molecular weight poly(vinyl chloride) (PVC), tetrahydrofuran (THF), acrylamide (AM) and ethylene glycol dimethacrylate (EGDMA) were obtained from Fluka (Ronkonoma, city, New York, USA). Benzoyl peroxide (BPO) was purchased from Riedel-deHaen. Acetonitrile, acetic acid, methanol, Tris (hydroxymethyl) amino methane (Trizma) and were purchased from Sigma Chemical Company (St. Louis, MO, USA).
Trizma buffer (5 mmol L⁻¹, pH 7.1) was prepared and used as a working buffer solution. A stock solution of 1.0 × 10^–1 mol L^–1 DMA⁺ was prepared and the working solutions (1.0 × 10⁻² to 1.0 × 10⁻⁵ mol L⁻¹) were also prepared by accurate dilutions.

S. M. Hassan S., E. Amr A.E., Abd El-Naby H., A. Al-Omar M., H. Kamel A, & Khalifa N.M. (2019). Potentiometric PVC-Membrane-Based Sensor for Dimethylamine Assessment Using A Molecularly Imprinted Polymer as A Sensory Recognition Element. Polymers, 11(10), 1695.

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Cultivation of Synechococcus and Phage S-PM2d

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Axenic cultures of Synechococcus sp. WH7803 and phage S-PM2d (Myoviridae) were kindly provided by Dr. Martha Clokie (University of Leicester, UK). Synechococcus strain was maintained at 23°C under continuous light (20–23 μE m^-2 s^-1) on artificial seawater medium (ASW) containing per L: 25 g NaCl; 2 g MgCl₂ x 6H₂O; 0.5 g KCl; 0.75 g NaNO₃; 0.002 g K₂HPO₄ x 3H₂O; 3.5 g MgSO₄ x 7H₂O; 0.5 g CaCl₂ x 2H₂O; 1.1 g Trizma buffer (Sigma), and 1 mL trace metal solution containing per L: 2.86 g 1H₃BO₃; 1.81 g MnCl₂ x 4H₂O; 0.222 g ZnSO₄x 7H₂O; 0.39 g Na2MoO₄ x 2H₂O; 0.008 g CuSO₄ x 5H₂O; 0.0049 g Co(NO₃)₂ x 6H₂O; 3.0 g FeCl₃ x 6H₂O and 0.5 g EDTA, as described in [16 (link),17 ] but with reduced phosphate concentration.
Clonal strain of the phage S-PM2d was cultured by picking a single plaque from a plaque assay and then re-suspending it in a liquid culture of Synechococcus sp. WH7803. The culture was spun down after host-lysis at 6000 rpm for 20 min to remove cell debris. The supernatant containing the phage particles (lysate) was then filtered (0.22 μm) to remove any remaining cells and stored at 4°C until addition into the chemostats.

Tjendra J., Storesund J.E., Dahle H., Sandaa R.A, & Våge S. (2023). Molecular evidence of parallel evolution in a cyanophage. PLOS ONE, 18(2), e0281537.

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Collagenase Degradation of Crosslinked Cartilage

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The effect of photochemical crosslinking on the resistance of cartilage to degradation by collagenase was investigated. Osteochondral specimens were treated with one of the two selected crosslinking protocols or were left untreated (n = 5 for each protocol). Using a sledge microtome (HM 450 Richard Allan, Kalamazoo, MI) equipped with a freezing stage (Physitemp, Clifton, NJ), three 50 μm sections were taken starting from the articular surface of each specimen. Individual sections were incubated for 45 minutes at 37 °C in 0.5 ml of a 2 mg/ml solution of type I collagenase from Clostridium histolyticum in 50 mM Trizma buffer at pH 7.4 containing 10 mM CaCl₂ (all from Sigma-Aldrich, St. Louis, MO).^{11 (link),12 (link)} To quantify the digested collagen, the digest solution for all sections were collected and assayed for HYP and the amount of digested collagen was estimated, as above.

Noori-Dokht H., Joukar A., Karnik S., Williams T., Trippel S.B, & Wagner D.R. (2022). A Photochemical Crosslinking Approach to Enhance Resistance to Mechanical Wear and Biochemical Degradation of Articular Cartilage. Cartilage, 13(3), 19476035221093064.

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Quantifying MMP-9, MMP-2, and Neutrophil Elastase

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Enzyme-linked immunosorbent assay (ELISA) for MMP-9 and MMP-2 in BM supernatant and plasma was performed using Quantikine ELISA kits (R&D Systems, Minneapolis, MN). Plates were read using Epoch7 96-well plate reader and the kinetic function on Gen5 software. Because of high cost and low availability of commercially prepared ELISA kits for neutrophil elastase in our species, an enzymatic assay of elastase (EC 3.4.1.36) (Sigma–Aldrich, St. Louis, MO) cleavage function was adapted for use in a 96-well plate with the following modifications: 10 µL of sample (BM supernatant or plasma), 120 µL of Trizma Buffer (12.1 mg/mL, pH 8.0 at 25°C) (Sigma–Aldrich), and 20 µL of N-succinyl-(Ala)₃-p-nitroanilide substrate (2 mg/mL; Sigma–Aldrich) were used per well. The pathlength for 150 µL in the 96-well Flat Bottom plate (Becton Dickinson Labware, Franklin Lakes, NJ) was experimentally determined and used to correct the millimolar coefficient (for 1 cm pathlength) provided in the protocol defined as units per milliliter. All samples were run in duplicate.

Doobay G.D., Miller E.S., Apple C.G., Loftus T.J., Kannan K.B., Efron P.A, & Mohr A.M. (2020). Mediators of Prolonged Hematopoietic Progenitor Cell Mobilization After Severe Trauma. The Journal of surgical research, 260, 315-324.

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Antioxidant and Elastase Inhibition

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The materials utilized include DPPH, quercetin, elastase from porcine pancreas (porcine pancreatic elastase or PPE), N-Succinyl-Ala-Ala-Ala-p-nitroanilide (elastase substrate), gallic acid, and Trizma buffer, which were obtained from Sigma-Aldrich, USA; ethanol, ammonia, chloroform, HCl, and ether were purchased from PT. Smart Lab Indonesia, Indonesia; acetic acid anhydrous, sulfuric acid, magnesium powder, amyl alcohol, FeCl₃, formaldehyde, C₂H₃NaO₃, and NaOH were purchased from PT. Brataco, Indonesia; petroleum ether, methanol pro analyze, and distilled water were purchased from PT. Ikapharmindo Putramas, Indonesia.

Ambarwati N.S., Sukma N.T., Desmiaty Y., Auliya A., Budi S., Arifuddin M, & Ahmad I. (2024). Garcinia dulcis and Garcinia forbesii King fruit peel extract: Secondary metabolite composition, antioxidant, and elastase inhibitory activity evaluation. Journal of Advanced Pharmaceutical Technology & Research, 15(1), 8-12.

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Quantitative Cell Viability Assay

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Cells were fixed by adding 33% TCA and kept for 1 h at 4°C. After washing with tap water, 0.4% SRB (Sigma) solution in 1% acetic acid (Sigma) was added to each well, and the plates were kept for 5 min at RT. After staining, the plates were washed with 1% acetic acid and air-dried. The bound stain was solubilized with 1% Trizma buffer (Sigma), and the absorbance of the dye in solution is measured at OD 515 nm.

Lee S., Jeong Y., Roe J.S., Huh H., Paik S.H, & Song J. (2021). Mitochondrial dysfunction induced by callyspongiolide promotes autophagy-dependent cell death. BMB Reports, 54(4), 227-232.

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