Dimension rxl max integrated chemistry system

Plasma was obtained from whole blood samples by centrifugation for 5 minutes at 2000 g and 4°C. Then, the levels of cholesterol, triglycerides, high-density lipoprotein (HDL), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, albumin, and urea were measured using the automated Dimension® RXL MAX Integrated Chemistry System (Siemens, USA).

Mice were fasted overnight for all blood analyses. For hematology, blood samples were collected in Microvette 100 EDTA tubes (20.1278, Sarstedt, Nümbrech, Germany) and analyzed with a PENTRA 80 hematology analyzer (Horiba, Kyoto, Japan). For lipid profile analysis, blood samples were collected in plastic tubes, incubated at room temperature (RT) to allow clotting, and centrifuged at 1900× g for 10 min (4 °C). Collected serum was centrifuged for 10 min at maximum speed (4 °C), harvested, and stored at −80 °C. When the serum sample volume was insufficient for the analysis, specimens from 2–3 mice of the same genotype were pooled. Hemolyzed specimens were excluded from testing. The serum content of total cholesterol, free cholesterol, HDL, and LDL was measured using a Dimension RxL Max Integrated Chemistry System (Siemens Healthineers, Erlangen, Germany). All analyses were performed by specialized staff at the CNIC Animal Facility.

Blood urea nitrogen (BUN), creatinine, total calcium, and phosphorus levels were determined in sera of all participants alongside the urinary calcium and phosphorus levels using Siemens Dimension RxL Max Integrated Chemistry System (Siemens Healthcare Diagnostics, DE, USA).

Lap times were obtained to measure the running speed (m/s) every 400 meters. After dividing the distance of entire ultramarathon race (100 km) into 10 splits, the mean and standard deviation (SD) of running speed in each 10 km-split (25 laps of 400 m oval track) were calculated for all runners. The variability of speed was determined by using the coefficient of variation (CV), which was defined as the ratio of the standard deviation to the mean of lap speeds [23 (link),26 (link),27 (link)].
Venous blood (20ml) was drawn antiseptically by a 20-gauge intravenous catheter 1 week before and immediately post-race to examine biochemical data, including blood urea nitrogen (BUN), creatinine (Cr), creatine kinase (CK), lactate dehydrogenase (LDH), myoglobin, uric acid (UA), electrolytes, D-dimer, Procalcitonin, and liver function tests. BUN and Cr were examined again one day after race to assess the recovery of renal function. All specimens were refrigerated and transported to the laboratory within 4 hours of sampling. Plasma samples were assayed on the Siemens Dimension RXL Max Integrated Chemistry System using reagents supplied by the manufacturer. Body weight (BW) change (the difference between before and 4 hours after the start) was also recorded to monitor the dehydrated status.