Anti mouse rat foxp3 apc

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Anti-Mouse/Rat Foxp3 APC is a flow cytometry reagent that detects the expression of the Foxp3 transcription factor in mouse and rat cells. Foxp3 is a marker for regulatory T cells (Tregs). The APC (Allophycocyanin) fluorochrome is used for labeling the anti-Foxp3 antibody, enabling its detection by flow cytometry.

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Lab products found in correlation

Lsrfortessa, by BD (1 mentions) Rat igg2a k isotype control apc, by Thermo Fisher Scientific (1 mentions) Golgiplug protein transport inhibitor, by BD (1 mentions) Irinotecan, by Merck Group (1 mentions) Tcdca, by Merck Group (1 mentions) Tαmca, by Steraloids (1 mentions) Cytofix cytoperm fixation permeabilization solution, by BD (1 mentions) Foxp3 fix perm buffer set, by BioLegend (1 mentions) T β mca, by Steraloids (1 mentions) T udca, by Steraloids (1 mentions) T hdca, by Steraloids (1 mentions) Anti mouse cd4 efluor450, by Thermo Fisher Scientific (1 mentions) Anti mouse cd45 alexa fluor 700, by Thermo Fisher Scientific (1 mentions) Anti mouse il 10 apc, by Thermo Fisher Scientific (1 mentions) Mouse il 10 elisa kit, by BioLegend (1 mentions) Facscanto, by BD (1 mentions) Fc block, by BD (1 mentions) Anti mouse f4 80 pe, by Thermo Fisher Scientific (1 mentions) Streptavidin fitc, by Thermo Fisher Scientific (1 mentions) Anti canine cd3, by Bio-Rad (1 mentions)

Spelling variants of this product

Anti-Foxp3 (119 citations) Foxp3-APC (77 citations) Anti-Foxp3-APC (68 citations) Rat anti-Foxp3 (10 citations) Rat anti-mouse Foxp3 (8 citations) Anti-mouse Foxp3 APC (8 citations) Anti-Mouse/Rat FoxP3 Staining Set APC (4 citations) Anti-Mouse/Rat-Foxp3-APC-(FJK-16s) (3 citations)

4 protocols using anti mouse rat foxp3 apc

Immune Cell Profiling by Flow Cytometry

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Peripheral blood mononuclear cell suspensions were analyzed using BD LSRFortessa custom flow cytometer (LSRFortessa SORP, Becton, Dickinson and Company), using the following antibodies: Rat CD4 APC-Cy7 OX-35, MS IGG2A KPA ITCL APC-CY7 G155-178, Rat IL-4 PE OX-81, Ms IgG1 Kpa ItCl PE MOPC-21, Rat IFN-Gma FITC DB-1, Ms IgG1 Kpa ItCl FITC MOPC-21, Rat CD25 BV421 OX-39, Mouse IgG1 Kpa ItCl BV421 X40 (BD Pharmingen, USA), Anti-Mouse/Rat IL-17A PerCP-Cyanine5.5, Rat IgG2a K Isotype Control PerCP-Cyanine5.5, Anti-Mouse/Rat Foxp3 APC, Rat IgG2a K Isotype Control APC (eBioscience, USA), and Transcription Factor Buffer Set, Leuko Act Cktl With GolgiPlug (BD Pharmingen, USA).The expression levels of Th1, Th2, Th17, and Treg cells were detected by the expression levels of CD3⁺CD4⁺IFN-γ⁺, CD3⁺CD4⁺IL-4⁺, CD3⁺CD4⁺IL-17A⁺, CD4⁺CD25⁺Foxp3⁺, respectively.

Peng J., Lu X., Xie K., Xu Y., He R., Guo L., Han Y., Wu S., Dong X., Lu Y., Liu Z., Cao W, & Gong M. (2019). Dynamic Alterations in the Gut Microbiota of Collagen-Induced Arthritis Rats Following the Prolonged Administration of Total Glucosides of Paeony. Frontiers in Cellular and Infection Microbiology, 9, 204.

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Bile Acid Panel Profiling Protocol

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β-MCA, ω-MCA, UDCA, HDCA, T-α-MCA, T-β-MCA, T-UDCA, and THDCA were purchased from Steraloids Inc. (Newport, RI). α-MCA, CA, DCA, CDCA, TCDCA, TDCA, and TCA were purchased from Sigma-Aldrich (St Louis, MO). Irinotecan was obtained from Sigma-Aldrich (St Louis, MO). All other reagents and solvents were of HPLC grade. Anti-mouse CD3 (clone 145-2C11), anti-mouse CD28 (clone 37.51), anti-mouse CD16/CD32 (clone 93), anti-mouse CD45 Alexa Fluor® 700 (Clone: 30-F11), anti-mouse CD4 eFluor® 450 (Clone: GK1.5), anti-mouse CD8α PE-Cyanine7 (Clone: 53-6.7), anti-mouse CD8β FITC (Clone: eBioH35-17.2), anti-mouse TCRβ PerCP-Cyanine5.5 (Clone: H57-597), anti-mouse/rat Foxp3 APC (Clone: FJK-16s), anti-mouse IL-10 APC (Clone: JES5-16E3) were from eBiosciences (San Diego, CA). Cytofix/Cytoperm Fixation/ Permeabilization Solution and Golgi Plug protein transport inhibitor were purchased from BD Biosciences (Franklin Lakes, NJ). Foxp3 Fix/Perm Buffer Set and mouse IL-10 ELISA kit were purchased from BioLegend (San Diego, CA).

Fang Z.Z., Zhang D., Cao Y.F., Xie C., Lu D., Sun D.X., Tanaka N., Jiang C., Chen Q., Chen Y., Wang H, & Gonzalez F.J. (2015). Irinotecan (CPT-11)-induced elevation of bile acids potentiates suppression of IL-10 expression. Toxicology and applied pharmacology, 291, 21-27.

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Multiparameter Flow Cytometry Analysis

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Isolated cells were incubated in 2% FBS and Fc block (BD Bioscience) for 20 min at 4°C and then stained with fluorescent-conjugated antibody. Most Abs used for flow cytometry analysis were purchased from BD Biosciences except for anti-mouse F4/80-PE (eBioscience), anti-mouse/rat Foxp3-APC (eBioscience), and anti-mouse CD317(BST2, PDCA-1)-PE (eBioscience). Data were obtained using FACSCanto (BD Bioscience) and analyzed with FlowJo software (Tree Star, Ashland, OR). For intracellular staining for IL-10, the cells were stimulated with PMA/ionomycin or anti-CD3 antibody and then fixing and permeabilization buffer were used as per manufacturer instructions.

Kim Y.I., Lee B.R., Cheon J.H., Kwon B.E., Kweon M.N., Ko H.J, & Chang S.Y. (2016). Compensatory roles of CD8+ T cells and plasmacytoid dendritic cells in gut immune regulation for reduced function of CD4+ Tregs. Oncotarget, 7(10), 10947-10961.

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Canine Leukocyte Isolation and Phenotyping

Cited 1 time

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Leukocytes were isolated from samples of canine brain tissue and peripheral blood using a previously established and published protocol^{20 –22 (link)} and single cell suspension was created. Cells were stained with anti-canine CD3 (Serotec, NC0522562) conjugated with anti-mouse IgG DyLight 549 (BioRad, STAR117D549GA), anti-canine CD4 PE/Cy7 (eBioscience, 25-5040-42), anti-canine CD8a eFluor 450 (eBioscience, 48-5080-42), anti-mouse/rat FoxP3 APC (eBioscience, 17-5773-82), anti-human CD152-FITC (MyBioSource.com, MBS666569), antihuman PD-1 biotinylated antibody (R&D Systems, BAF1086) conjugated with streptavidin FITC (eBioscience, 11-4317-87), anti-canine CD45 eFluor 450 (eBioscience, 48-5450-42), anticanine CD11c (BioRad, MCA17785) conjugated with anti-mouse IgG FITC (BioRad, STAR117F), anti-human CD123 PE (eBioscience, 12-1239-41), anti-human CD83 APC (MACS, 130-098-889), and anti-human PD-L1 (CD274) PE/Cy7 (BD Bioscience, BDB558017). Cells were analyzed by Flowcytometry, using a BD LSRFOrtessa X-20 cell analyzer, and data was analyzed with FlowJo V.10.2 software.

Filley A., Henriquez M., Bhowmik T., Tewari B.N., Rao X., Wan J., Miller M.A., Liu Y., Bentley R.T, & Dey M. (2018). Immunologic and Gene Expression Profiles of Spontaneous Canine Oligodendrogliomas. Journal of neuro-oncology, 137(3), 469-479.

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