The transplantation experiment was carried out according to previous reports with modifications (27 (link), 28 (link)). Mice at 3 weeks of age were treated with a combination of metronidazole (1 g/L), neomycin sulfate (1 g/L), ampicillin (1 g/L), and vancomycin (0.5 g/L) (Sangon Biotech) for 2 weeks. Then, at 5 weeks of age, the water was replaced by antibiotic-free water and the mice were administered (i.g.) with a mixture of 2 × 108 CFU Bifidobacterium longum (ATCC15697) and 2 × 108 CFU Bifidobacterium adolescentis (ATCC15703). Also, the antibiotic-free drinking water was supplied with B. longum (final concentration ≈106 CFU/mL) and B. adolescentis (final concentration ≈106 CFU/mL). Mice in the control group were administered (i.g.) with an equal volume of Modified Reinforced Clostridial Medium (BD 218081) but without bacterial cells and the antibiotic-free drinking water was also supplied with an equal volume of Modified Reinforced Clostridial Medium. Mice were evaluated after 15 days.
Neomycin sulfate
Manufactured by Sangon
Sourced in China
Neomycin sulfate is a powdered salt form of the antibiotic neomycin. It is a broad-spectrum aminoglycoside antibiotic used for various laboratory and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Metronidazole, by Sangon (6 mentions)
Ampicillin, by Sangon (5 mentions)
Vancomycin, by Sangon (5 mentions)
Bifidobacterium adolescentis, by American Type Culture Collection (1 mentions)
Bifidobacterium longum, by American Type Culture Collection (1 mentions)
Vancomycin hydrochloride, by Sangon (1 mentions)
Ampicillin sodium, by Sangon (1 mentions)
Streptomycin, by Sangon (1 mentions)
6 protocols using neomycin sulfate
1
Bifidobacterium Gut Modulation in Mice
2
Gut Microbiome Modulation by Antibiotic Cocktail
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Commensal bacteria from the gut were depleted using four antibiotics according to method reported before (26 (link)) with modifications. Briefly, for combinatorial antibiotic treatment, mice were provided with sterile water supplemented with metronidazole (1 g/L), neomycin sulfate (1 g/L), ampicillin (1 g/L), and vancomycin (0.5 g/L) (Sangon Biotech, Shanghai, China) beginning at 3 weeks of age and lasting for 5 weeks. For single antibiotic treatment, mice were treated with sterile water supplemented with each antibiotic alone at the dose indicated above. Water containing antibiotics was supplied as drinking water to mice and changed twice a week.
3
Comparison of Pharmaceutical Compounds
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METH was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China), with a purity＞99.0%. Vancomycin, neomycin sulfate, metronidazole, and ampicillin were obtained from Sangon Biotech (Shanghai, China).
4
PCOS Induction and Antibiotic Treatment
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Eighteen rats were randomly allocated to 3 groups: PBS, DHEA and DHEA + antibiotics groups (n = 6 each group). To induce the PCOS phenotype, rats in the DHEA group were subcutaneously injected with 6 mg/100 g bodyweight DHEA (cat. no. H10940064, Yangzhou Pharmaceutical Co., Ltd) daily for 35 consecutive days. Rats in the PBS group were injected with PBS. Rats in the DHEA + antibiotics group were injected with DHEA in the same manner as the DHEA group and received a freshly prepared antibiotic cocktail daily in their drinking water. The antibiotic cocktail, consisting of ampicillin sodium (1 mg mL−1), neomycin sulfate (1 mg mL−1), metronidazole (1 mg mL−1), and vancomycin hydrochloride (0.5 mg mL−1) (all purchased from Sangon Biotech, China) [54 (link)], was administered to rats every day immediately after preparation. The rats in all 3 groups were sacrificed after 5 weeks of treatment.
5
Neutralization and Depletion Strategies for Influenza
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For in vivo neutralization, the following neutralizing antibodies were administered: 100 µg/mouse anti–IL-17A (TC11-18H10.1), 100 µg/mouse anti-CCL25 (89818), 100 µg/mouse anti–IL-6 (MP5-20F3), or 100 µg/mouse anti–IL-15 (AIO.3) were administered into mice at days 0, 2, and 4 after PR8 infection. For in vivo cell depletion, 200 µg/mouse anti-NK1.1 was administered i.v. into mice 2 d before PR8 infection. For intestinal microbiota depletion, mice were treated with a mixture of antibiotics (1 mg/ml ampicillin, 0.5 mg/ml vancomycin, 1 mg/ml neomycin sulfate, and 1 mg/ml metronidazole [Sangon Biotech]) added to their drinking water beginning 4 wk before PR8 infection and continuing until sacrifice, as previously described (Ichinohe et al., 2011 (link)). For intestinal E. coli depletion, mice were treated with 1 mg/ml streptomycin (Sangon Biotech) added to their drinking water beginning 1 wk before PR8 infection and continuing until sacrifice. Antibiotic-containing water was changed twice a week.
6
Antibiotic-induced Gut Microbiome Modulation
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At the beginning of the 5th week of CD or HSD supplementation, the mice were provided with drinking water containing an antibiotic cocktail (ampicillin, 1 g/L; neomycin sulfate, 1 g/L; metronidazole, 1 g/L; and vancomycin, 500 mg/L; Sangon Biotech (Shanghai) Co., Ltd.) for 4 weeks ad libitum in the meantime (27 (link)). The antibiotics were renewed every other day. After 4 weeks of antibiotic treatment, the mice were treated with zymosan A, and the peritoneal fluid was collected for further experiments.
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