2 hydroxyethyl methacrylate

2-hydroxyethylmethacrylate (HEMA), 2-Aminoethyl methacrylate hydrochloride (AEM) N,N′-methylenebisacrylamide (MBAA), ammonium persulphate (APS), 1,2-bis(dimethylamino)ethane (TEMED) and sodium chloride, and cecropin B and cecropin P1 were purchased from Merck KGaA (Darmstadt, Germany). Phosphate buffer saline (PBS) was purchased from Gibco Italia. Peptone, meat extract, tryptone, yeast extract and Noble Agar were purchased from BD Diagnostics (Franklin Lakes, NJ, USA). Antibodies against E. coli (Polyclonal anti-E. coli ab13627) were purchased from Abcam (Cambridge, UK). Quantum dots (QDs) and Qdot™ Incubation Buffer were purchased from Invitrogen (code Q10101 MP and Q20001 MP, respectively).

Dopamine hydrochloride (DA, 98%), epinephrine hydrochloride (EP, 98%), norepinephrine hydrochloride (NE, 98%), (L)-phenylalanine methyl ester, (L)-cysteine methyl ester, ethylene glycol dimethacrylate (EDMA), 2-hydroxyethyl methacrylate (HEMA), methanol, and acetic acid were supplied from Merck (Darmstadt, Germany). The chemicals used to prepare buffers in this research were sodium dihydrogen phosphate adjusted with phosphoric acid to obtain a pH of 1.5, acetic acid and sodium acetate for the preparation of acetate buffer to obtain a pH of 5.5, and potassium phosphate monobasic and potassium phosphate dibasic for the preparation of phosphate buffer to achieve a pH of 7.4. Ammonium chloride was adjusted by ammonia to obtain a pH of 8.5, and, finally, carbonic acid and sodium hydroxide adjusted the pH to 10.5, which were obtained from Merck (Darmstadt, Germany). Deionized (DI) water (18.2 MΩ cm) was utilized to make all of the aqueous solutions and treated with pure lab Ultra Analytic (ELGA Lab Water, Runcorn, UK). The pH meter was used to measure the pH, and the ultrasonic water bath was also used in solution preparation. Pencil graphite electrodes (PGEs) were used in pencil tips (Tombow 0.5, HB) obtained from local stationery stores.

2-Hydroxyethyl methacrylate, and 4-chloromethyl styrene (Merck, Darmstadt, Germany) were dried over calcium hydride, vacuum-distilled, and then stored at −20 °C prior to use. The initiator 2,2′-azobisisobutyronitrile (AIBN, Fluka, Switzerland) was recrystallized from ethanol at 50 °C before use. Tetrahydrofuran (THF, Merck) was dried by refluxing over sodium, and distilled under argon atmosphere before use. Diethyl amine, 4,4′-azobis(4-cyanopentanoic acid), bromobenzene, and pyridine were purchased from Sigma-Aldrich (USA), and were used as received. All other reagents were purchased from Merck and purified according to the standard methods.

Dimethylsulfoxide (DMSO, Riedel-de Haen, Seelze, Germany) was used without any further purification. Silver nitrate (AgNO₃,) was purchased from Degussa (Berlin, Germany). For lens preparation, 2-hydroxyethyl-methacrylate (pHEMA), ethylene-glycole-dimethacrylate (EGDMA, MERCK, Germany), diphenyl-(2,4,6-trimethylbenzoyl)-phosphine oxide (TPO 97%, Sigma-Aldrich, Steinheim, Germany) as well as sodium chloride (NaCl, MERCK) and hydrochloric acid (HCl 37%, MERCK) were used. Tryptone tryptophan medium, beef extract powder, peptone bacteriological and soy peptone were purchased from Biolife. Agar (Louis, MO, USA, product of Spain) and yeast extracts were purchased from Fluka Analytical (Switzerland). D(+)-glucose and di-potassium hydrogen phosphate trihydrate were purchased from Merck. Melting points were measured in open tubes with a Stuart Scientific apparatus and were uncorrected. A UV-1600 PC series spectrophotometer of VWR was used to obtain electronic absorption spectra. ATR-FT-IR spectra in the region of 4000–370 cm⁻¹ were obtained with a Cary 670 FTIR spectrometer (Agilent Technologies Inc., Santa Clara, CA, USA). XRF measurement was carried out with Rigaku NEX QC EDXRF analyzer, (Austin, TX, USA).

For membrane preparation, n-butyl acrylate (nBA) from Merck, 2-hydroxyethyl methacrylate (HEMA), 1,6-hexanediol diacrylate (HDDA), 2-dimethoxy-2-phenyl acetophenone (DMPP), sodium tetrakis [3,5-bis(trifluoromethyl) phenyl] borate (NaTFPB), potassium tetrakis-(4-chlorophenyl)borate (KTClPB) and potassium ionophore I from Sigma Aldrich (St. Louis, MO, USA) were used. The solvents and the metal salts included potassium chloride, copper (II) chloride dihydrate, calcium chloride and sodium chloride from Sigma Aldrich, cadmium chloride and aluminium chloride from Acros, mercury chloride from Fisher, zinc chloride and nickel chloride hexahydrate from Merck, iron(III) chloride from R&M Chemicals, tetrahydrofuran from SYSTERM and magnesium nitrate from Bendosan. Lithium acetate dihydrate from Acros, tris(hydroxymethyl)aminomethane hydrochloride, Tris-HCl, and silver chloride from Sigma Aldrich and bacto agar from Difco used for conventional reference electrode preparation. The chemicals used were the aluminium ionophore 1,1′-[(methylazanediyl)bis(ethane-2,1-diyl)]bis[3-(naphthalen-1-yl)thiourea], ACH synthesized and purified according to Kook et al. [34 (link)] and Abosadiya et al. [35 ]. All analytical grade chemicals were used as obtained. Deionized water (specific resistance, 18.0MΩ cm) was utilized throughout this work.

Priplast 1838 (dimer linoleic acid based polyester diol) was kindly provided by Cargill Bioindustrial (The Netherlands). Isophorone diisocyanate (98%, mixture of isomers) (IPDI) and 2-hydroxyethylmethacrylate (97%) (HEMA) were purchased from Merck KGaA (Germany). Phenothiazine (PTZ) was purchased from Sigma Aldrich (Poland). Photoinitiator Omnirad 819 was purchased from IGM resins (The Netherlands). Ethyl acetate (EtOAc) and methanol were purchased from Chempur (Poland). HEMA was used after distillation under reduced pressure; all other reagents were used as received. Magnesium chips and titanium(iv) butoxide were purchased from Sigma Aldrich (Poland), and n-butanol was purchased from Eurochem BGD (Poland). Murine fibroblast cell line, L929, as well as all cell culture reagents were purchased from Sigma Aldrich (Poland). All disposable cell culture plasticware was purchased from VWR (Poland).