1100 ion trap msd spectrometer

4-Bromo-1,8-naphthalic, morpholine, 2-methoxyethanol, ethylenediamine, chloroacetyl chloride, trimethylamine, hydrazine monohydrate, benzotriazol-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate, and anhydride were bought from Innochem (Beijing, China). The other reagents were purchased from J&K Chemicals. All reagents were analytical level and employed without further purification. Nitrate salts of metal ions (Cd²⁺, Ni²⁺, Co²⁺, Ba²⁺, Ca²⁺, Pb²⁺, Fe³⁺, Cu²⁺, Mg²⁺, Mn²⁺, Zn²⁺, K⁺, Sr²⁺, Cr³⁺, Al³⁺, Ag⁺) and the perchlorate salt of Hg²⁺ were used for appraising the combining capacity of ions to compounds. Deionized water was applied to the entire experiment.
¹H and ¹³C NMR spectra were obtained on a Bruker AV400 NMR spectrometer using CD₂Cl₂ as the solvent and tetramethylsilane (TMS) as an internal reference. Mass spectrometry was conducted on an Agilent 1100 ion trap MSD spectrometer. HPLC spectra were taken on an Agilent 1260. An Agilent UV-8454 spectrophotometer was applied to the absorption measurements. The fluorescence spectra were collected using a Hitachi F-4500 fluorescence spectrophotometer. The melting points were determined by a WRS-1B melting point apparatus. The staining of cells was performed using an OLYMPUS FV1000 confocal laser scanning microscope. The fluorescence quantum yield value was recorded by a machine named QY C11347-11.

NMR spectra were obtained by a Bruker AV400 (¹H NMR 400 MHz, ¹³C NMR 100 MHz) instrument using CD₂Cl₂ and DMSO-d₆ as solvent and tetramethylsilane (TMS) as the internal standard. Mass spectrometry analysis was performed on an Agilent 1100 ion trap MSD spectrometer. Melting points were measured with a WRS-1B melting point apparatus. Fluorescence spectra were collected with a Hitachi F-4600 fluorescence spectrophotometer. UV-vis absorption spectra were carried out using an Agilent 8453 UV/vis spectrophotometer. Infrared spectra (IR) were collected on a Bruker Vertex-70 spectrometer. Elemental analysis was determined on a PE CHN 2400 analyzer. Fluorescence imaging of cells was performed using an Olympus FV1000 confocal laser scanning microscope.