Log In Sign up
The largest database of trusted experimental protocols

On targetplus smartpool sirna

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

ON-TARGETplus SMARTpool siRNA is a collection of pre-designed small interfering RNA (siRNA) sequences targeting a specific gene. The product is designed to provide efficient and specific gene silencing.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (14 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (8 mentions) Dharmafect 1 transfection reagent, by Thermo Fisher Scientific (3 mentions) Opti mem, by Thermo Fisher Scientific (3 mentions) Dharmafect 1, by Thermo Fisher Scientific (3 mentions) Amaxa nucleofector 2, by Lonza (3 mentions) Dharmafect 1 reagent, by Thermo Fisher Scientific (2 mentions) On targetplus non targeting pool, by Thermo Fisher Scientific (2 mentions) Non targeting pool, by Thermo Fisher Scientific (2 mentions) On targetplus smartpool sirna, by Horizon Discovery (2 mentions) On targetplus non targeting pool sirna, by Thermo Fisher Scientific (2 mentions) Nucleofector solution c, by Lonza (2 mentions) Rnaimax, by Thermo Fisher Scientific (2 mentions) On targetplus non targeting pool sirna, by Horizon Discovery (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Hdac6, by Thermo Fisher Scientific (1 mentions) Cell line nucleofector kit 5, by Lonza (1 mentions) Amaxa human t cell nucleofector kit, by Lonza (1 mentions) Amaxa cell line nucleofector kit t, by Lonza (1 mentions) Attractene reagent, by Qiagen (1 mentions)

69 protocols using on targetplus smartpool sirna

1

Transcription Factor Regulation of Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tfe3, Mitf, and Tfeb were knocked down independently by small interference RNA (siRNA) transfected into differentiated C2C12 and primary B6 cells. These experiments were performed using four pools of siRNA (ON-TARGET plus SMART pool siRNA), and as the control non-targeting siRNA pool (Thermo Scientific, Waltham, MA). After 72 h, RNA and protein was obtained for RT-PCR and Western blot analysis.
Of note, Tfe3, Mitf, and Tfeb knockdown vectors did not exhibit any sequence cross reactivity.
SALMA N., SONG J.S., ARANY Z, & FISHER D.E. (2015). Transcription Factor Tfe3 Directly Regulates Pgc-1alpha in Muscle. Journal of cellular physiology, 230(10), 2330-2336.
+ Open protocol
+ Expand
2

siRNA Knockdown with ON-TARGET plus

Check if the same lab product or an alternative is used in the 5 most similar protocols
ON-TARGETplus SMARTpool siRNA (Dharmacon / Thermo Scientific) was used for all siRNA knockdown experiments. Cells were transfected with the selected ON-TARGETplus SMARTpool siRNA constructs (25 nM final concentration), DharmaFECT 1 reagent (Thermo Scientific), and Opti-MEM (Life Technologies) according to Thermo Scientific’s recommendations.
The following Dharmafect siRNA targets were used:
Humpton T.J., Alagesan B., DeNicola G.M., Lu D., Yordanov G.N., Leonhardt C.S., Yao M.A., Alagesan P., Zaatari M.N., Park Y., Skepper J.N., Macleod K.F., Perez-Mancera P.A., Murphy M.P., Evan G.I., Vousden K.H, & Tuveson D.A. (2019). Oncogenic Kras induces Nix-mediated mitophagy to promote pancreatic cancer. Cancer discovery, 9(9), 1268-1287.
+ Open protocol
+ Expand
3

Transient Transfection and Stable Cell Line Creation

Check if the same lab product or an alternative is used in the 5 most similar protocols
MNT-1 cells were transiently transfected with 100 nM ON-TARGETplus SMARTpool siRNA (Thermo Fisher Scientific, Waltham, MA, USA) using Lipofectamine RNAimax (Invitrogen) according to the manufacturer’s instructions. For the shRNA-expressing stable cell line, a MATP shRNA plasmid was constructed using the MATP sequence (5’-GUACGAGUAUGGUUCUAUC-3’). After transfection with this plasmid, selection was performed for 2 weeks with 200–1000 μg/ml zeocin (Genolution, Seoul, Korea) according to the manufacturer’s instructions.
Bin B.H., Bhin J., Yang S.H., Shin M., Nam Y.J., Choi D.H., Shin D.W., Lee A.Y., Hwang D., Cho E.G, & Lee T.R. (2015). Membrane-Associated Transporter Protein (MATP) Regulates Melanosomal pH and Influences Tyrosinase Activity. PLoS ONE, 10(6), e0129273.
+ Open protocol
+ Expand
4

siRNA Transfection Protocol Using Amaxa

Check if the same lab product or an alternative is used in the 5 most similar protocols
For small interfering RNA (siRNA) transfection, scrambled, HDAC6 and cereblon (CRBN) ‘ON-TARGETplus SMARTpool siRNA' were purchased from Thermo Scientific (Lafayette, CO, USA). siRNA transfection was carried out by Amaxa electroporation system using ‘Cell Line Nucleofector Kit V' solution, according to manufacturer's protocol (Lonza, Koln, Germany).
Hideshima T., Cottini F., Ohguchi H., Jakubikova J., Gorgun G., Mimura N., Tai Y.T., Munshi N.C., Richardson P.G, & Anderson K.C. (2015). Rational combination treatment with histone deacetylase inhibitors and immunomodulatory drugs in multiple myeloma. Blood Cancer Journal, 5(5), e312-.
+ Open protocol
+ Expand
5

CD8+ T Cell Transfection and Depletion

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freshly isolated CD8+ T cells were transfected with 4mM ITK or 14-3-3z ON-TARGETplus SMARTpool siRNA or the ON-TARGETplus Non Targeting siRNA #1 (negative control) (ThermoScientific, Dharmacon) using the Amaxa Human T Cell Nucleofector kit (Lonza), as previously described (7 (link)). ITK or 14-3-3z depletion was determined 48 hours after nucleofection by WB.
Cascio S., Medsger TA J.r., Hawse W.F., Watkins S.C., Milcarek C., Moreland L.W., Lafyatis R.A, & Fuschiotti P. (2017). 14-3-3z sequesters cytosolic T-bet, up-regulating IL-13 in Tc2 and scleroderma CD8+ lymphocytes. The Journal of allergy and clinical immunology, 142(1), 109-119.e6.
+ Open protocol
+ Expand
6

Electroporation Silences CK1α in Myeloma Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Electroporation of INA-6 and H929 was performed as described in [50 (link)] with siGLO Green scrambled siRNAs and CK1α targeting siRNAs ON-TARGETplus SMARTpool siRNA (Thermo Scientific, USA). CK1α target sequences were: GCGAUGUACUUAAACUAUU; GGAAUCAUUAGGAUAUGUU; AGAGUAACAUGA AAGGUUU; GGCUAAAGGCUGCAACAAA. Cells were harvested at 72 h after nucleofection.
Manni S., Carrino M., Manzoni M., Gianesin K., Nunes S.C., Costacurta M., Tubi L.Q., Macaccaro P., Taiana E., Cabrelle A., Barilà G., Martines A., Zambello R., Bonaldi L., Trentin L., Neri A., Semenzato G, & Piazza F. (2017). Inactivation of CK1α in multiple myeloma empowers drug cytotoxicity by affecting AKT and β-catenin survival signaling pathways. Oncotarget, 8(9), 14604-14619.
+ Open protocol
+ Expand
7

Inducible MELK Knockdown in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
siRNA experiments utilized ON-TARGET plus SMARTpool siRNA (Thermo Scientific) targeting MELK or nontargeting control (nontargeting pool, catalog no. D-001810–10-50, Invitrogen) according to the manufacturer’s instructions. The pTRIPZ lentiviral system with MELK-inducible shRNA transfection starter kit was purchased from Thermo Scientific using cat #RHS4696–200703132 and cat #RHS4696–200691582 for non-template control and shMELK. Stable cell lines were generated using lentiviral transduction. Clones were selected and screened for both RFP and MELK expression changes and were used as pools and as selected stable clones in all in vitro and in vivo experiments.
Speers C., Zhao S.G., Kothari V., Santola A., Liu M., Wilder-Romans K., Evans J., Batra N., Bartelink H., Hayes D.F., Lawrence T.S., Brown P.H., Pierce L.J, & Feng F.Y. (2016). Maternal Embryonic Leucine Zipper Kinase (MELK) as a Novel Mediator and Biomarker of Radioresistance in Human Breast Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(23), 5864-5875.
+ Open protocol
+ Expand
8

Modulating NFATc1 Expression in Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNAi studies were performed as previously described using NFATc1
specific ON-target plus SMART pool siRNA or ON-target plus Non-targeting Pool
(Thermo Scientific; Rockford, IL,) Specific si-RNA sequences are given in Supplementary Table
4.
For preparing stable over-expressing cells, mouse wild-type NFATc1
(Addgene plasmid 11101) (20 (link)) was cloned
into vector pGP-Lenti3 (GenBank Accession no. JX861384) between unique XbaI and
BamHI sites. Puromycin and GFP expression were used as selection markers for
creation of stable cell lines.
For gene knockdown experiments, NFATc1 specific GIPZ lentiviral shRNAmir
(V3LMM_418820) (Thermoscientific) was selected for experimental use. Briefly,
MC38 cells were electroporated using NEON (Invitrogen). Puromycin and GFP
expression were used as selection markers for creation of stable cell lines. For
HT29 studies, cells were transiently co-transfected with pEF6-NFATc1 and pMaxGFP
vectors, flow sorted to enrich for highly expressing GFP-positive cells and
confirmed NFATc1 overexpression by Western blot.
Tripathi M.K., Deane N.G., Zhu J., An H., Mima S., Wang X., Padmanabhan S., Shi Z., Prodduturi N., Ciombor K.K., Chen X., Washington M.K., Zhang B, & Beauchamp R.D. (2014). NFAT transcriptional activity is associated with metastatic capacity in colon cancer. Cancer research, 74(23), 6947-6957.
+ Open protocol
+ Expand
9

Modulating Antiviral Responses via siRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
ON-TARGET plus SMART pool siRNA targeting human DDX58, IRF3 or Non-targeting Pool (Thermo Fisher Scientific Inc, Pittsburgh, PA) were transfected into A549 cells by electroporation using Amaxa Cell Line Nucleofector Kit T (Lonza Walkersville, Inc., Walkersville, MD) according to the manufacturer’s protocol. At 24 h post transfection, cells were seeded into 12-well plates. At 1.5 days post transfection cells were mock-infected or infected with Candid#1.
Kolokoltsova O.A., Grant A.M., Huang C., Smith J.K., Poussard A.L., Tian B., Brasier A.R., Peters C.J., Tseng C.T., de la Torre J.C, & Paessler S. (2014). RIG-I Enhanced Interferon Independent Apoptosis upon Junin Virus Infection. PLoS ONE, 9(6), e99610.
+ Open protocol
+ Expand
10

Evaluating SURVIVIN Knockdown Synergy

Check if the same lab product or an alternative is used in the 5 most similar protocols
ON-TARGETplus SMARTpool siRNA targeting human SURVIVIN was purchased from Thermo Scientific. These RNAi molecules are designed to target 4 different regions of SURVIVIN (target sequences: 5′-CAAAGGAAACCAACAAUAA-3′, 5′-GCAAAGGAAACCAACAAUA-3′, 5′-CACCGCAUCUCUACAUUCA-3′, 5′-CCACUGAGAACGAGCCAGA-3′). Transfection of siRNA into cells was done following the manufacturer recommended protocol. At 24 hr post transfection, the transfected cells were seeded onto 24-well plate. ADI-PEG20 or cisplatin alone or combination was added, at the end of 72 hr. Viable cells were counted using trypan blue exclusion.
Savaraj N., Wu C., Li Y.Y., Wangpaichitr M., You M., Bomalaski J., He W., Kuo M.T, & Feun L.G. (2015). Targeting argininosuccinate synthetase negative melanomas using combination of arginine degrading enzyme and cisplatin. Oncotarget, 6(8), 6295-6309.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!