The A673 and TC252 cells were cultured in serum-free medium following 4 h of transfection and were cultured for 24 h, prior to the addition of complete medium. The A673 and TC252 cells were washed twice with cold PBS 48 h following transfection and were subsequently fixed in cold 70% alcohol overnight. The fixed cells were incubated in propidium iodide and RNase A, and were detected by flow cytometry using a C6 Flow Cytometer® Instrument (BD Biosciences, Franklin Lakes, NJ, USA).
C6 flow cytometer instrument
Manufactured by BD
Sourced in United States
The C6 Flow Cytometer® Instrument is a compact, benchtop flow cytometer designed for multicolor analysis of cell samples. It utilizes laser-based technology to detect and analyze various cellular parameters, including size, granularity, and the expression of specific fluorescently-labeled markers. The instrument provides researchers with a tool for rapid and precise quantification of cell populations within a heterogeneous sample.
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Lab products found in correlation
4 protocols using c6 flow cytometer instrument
1
Cell Cycle Analysis by Flow Cytometry
2
Apoptosis Analysis of Transfected HepG2 Cells
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HepG2 cells were harvested after transfection with combination therapy constructs and washed twice using cold PBS containing 0.5% BSA. They were then resuspended in 1×Binding Buffer at a concentration of 1×106 cells/ml, and 100μl of the solution (1×105 total cells) were transferred to a 5 ml culture tube. Next, 5μl of FITC Annexin V and 5μl PI were added, and the cells were gently vortexed and then incubated for 15 min at RT (25°C) in the dark. A total of 400μl of 1×Binding Buffer was added to each tube, and the samples were analyzed by flow cytometry within 1 hr using a C6 FlowCytometer® Instrument (BD Biosciences, San Jose, CA, USA).
3
ZIKV-Specific T Cell Responses in Mice
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Four groups of 5-weeks-old A129 mice (n = 4) were immunized with DMEM, 1 × 105 FFU VSRD-ZIKV, 1 × 105 FFU ZIKV MR766 WT, or 1 × 105 FFU ZIKV PRVABC59 through the s.c. route. On day seven p.i., all animals were sacrificed, and the spleens were isolated to perform the ICS experiment. Approximately 2 × 106 splenocytes were stimulated with E polypeptide array (strain PRVABC59) (Bioresource) overnight. At the same time, BD GolgiPlug (BD Bioscience, San Jose, CA, USA) was added to block protein transport. Cells were incubated with antibodies to CD4 or CD8, then fixed with 2% paraformaldehyde. Cells were permeabilized before the addition of anti-interferon-γ (IFN-γ) or control IgG1 (rats) (e-Biosciences). All samples were processed with a C6 Flow Cytometer instrument (BD Biosciences). Dead cells were excluded based on the forward and side scatter. Data were analyzed with FlowJo.
4
Phenotypic Analysis of Differentiated HL-60 Cells
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HL-60 cells were harvested at 48 h after transfection and were washed twice at 4°C in PBS containing 0.5% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.). ATRA- or PMA-induced HL-60 cells were incubated with phycoerythrin-conjugated cluster of differentiation molecule 11B (CD11b) antibody (CD11b04-4; eBioscience; Thermo Fisher Scientific, Inc.) or fluorescein isothiocyanate-conjugated anti-cluster of differentiation CD14 antibody (11-0141-82; eBioscience; Thermo Fisher Scientific, Inc.) at 1:100 dilution for 30 min at room temperature. Flow cytometry was performed using a C6 flow cytometer instrument (BD Biosciences, Franklin Lakes, NJ, USA) and subsequently analyzed with CFlow Sampler Analysis 1.0.208.2 (BD Biosciences).
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