Rabbit anti human mcl 1 monoclonal antibody

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-human Mcl-1 monoclonal antibody is a laboratory reagent produced by Cell Signaling Technology. It is designed to detect the Mcl-1 protein, which is a member of the Bcl-2 family of proteins involved in the regulation of apoptosis.

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Lab products found in correlation

Enhanced chemiluminescence, by Cytiva (1 mentions) Mouse anti human α tubulin clone dm1a monoclonal antibody, by Cell Signaling Technology (1 mentions)

2 protocols using rabbit anti human mcl 1 monoclonal antibody

Western Blot Analysis of Apoptosis Regulators

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HACC cells (UM-HACC-2A,-2B,-5,-6) lysates were resolved by electrophoresis and membranes were probed overnight at 4°C with a 1:1,000 dilution of hamster anti-human Bcl-2 monoclonal antibody (BD Biosciences, San Jose, CA, USA), rabbit anti-human Mcl-1 monoclonal antibody (Cell Signaling, Beverly, MA, USA) or mouse anti-human Bcl-x_L monoclonal antibody (BD Biosciences) for 1 hour at room temperature. Membranes were exposed to appropriate peroxidase-coupled secondary antibodies and proteins were visualized with enhanced chemiluminescence (Amersham, Pittsburgh, PA, USA).

Acasigua G.A., Warner K.A., Nör F., Helman J., Pearson A.T., Fossati A.C., Wang S, & Nör J.E. (2015). BH3-mimetic small molecule inhibits the growth and recurrence of adenoid cystic carcinoma. Oral oncology, 51(9), 839-847.

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Apoptotic Protein Expression Analysis

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The following antibodies were used: rabbit anti-human BCL-2 (clone D55G8) monoclonal antibody (1:1,000, Cell Signaling Technology); rabbit anti-human BCL-X_L (clone 54H6) monoclonal antibody (1:1,000, Cell Signaling Technology); rabbit anti-human MCL-1 monoclonal antibody (1:1,000, Cell Signaling Technology); rabbit anti-human BCL-W (Clone 31H4) monoclonal antibody (1:1,000, Cell Signaling Technology); rabbit anti-human Noxa (clone EPR9735B) monoclonal antibody (1:1,000, Abcam); rabbit anti-human BIM (1:1,000, Cell Signaling Technology); mouse anti-human cytochrome c (clone 7H8.2C12) monoclonal antibody (1:1,000 BD pharmingen); rabbit anti-human PARP (Clone 9542S) monoclonal antibody (1:1,000, Cell Signaling Technology); rabbit anti-human cleaved caspase 3 (Clone 5AE17) monoclonal antibody (1:1000, Cell Signaling Technology); mouse anti-human α-tubulin (clone DM1A) monoclonal antibody (1:10,000, Cell Signaling Technology) and horseradish peroxidase (HRP)-conjugated goat anti-rabbit (clone NA9340V) and goat anti-mouse (clone NXA931) secondary antibodies (1:10,000 GE Healthcare). See Supplementary Materials and Methods for detailed protocol.

Bate-Eya L.T., den Hartog I.J., van der Ploeg I., Schild L., Koster J., Santo E.E., Westerhout E.M., Versteeg R., Caron H.N., Molenaar J.J, & Dolman M.E. (2016). High efficacy of the BCL-2 inhibitor ABT199 (venetoclax) in BCL-2 high-expressing neuroblastoma cell lines and xenografts and rational for combination with MCL-1 inhibition. Oncotarget, 7(19), 27946-27958.

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