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Potassium nitrosodisulfonate

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Potassium nitrosodisulfonate is a chemical compound used as a laboratory reagent. It is a white, crystalline solid that is soluble in water. The compound is used in various analytical and research applications, but no further details about its intended use can be provided in an unbiased and factual manner.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Mitosox red, by Thermo Fisher Scientific (1 mentions) Penicillin, by American Type Culture Collection (1 mentions) Streptomycin, by American Type Culture Collection (1 mentions) Trifluoroacetic acid anhydride, by Merck Group (1 mentions) Human hb, by Merck Group (1 mentions) Chloroform, by Merck Group (1 mentions) L ascorbic acid, by Merck Group (1 mentions) N acetyl l cysteine, by Merck Group (1 mentions) Methanesulfonic acid, by Merck Group (1 mentions) Acetonitrile, by Tedia (1 mentions) Acetone, by Tedia (1 mentions) Ethyl acetate, by Tedia (1 mentions) Methyl alcohol, by Tedia (1 mentions) Thiazole orange, by Merck Group (1 mentions) Ammonium acetate, by Thermo Fisher Scientific (1 mentions) Menadione, by Merck Group (1 mentions) Dihydroethidium, by Merck Group (1 mentions)

Close spelling variants of this product

Fremy's salt (potassium nitrosodisulfonate, (2 citations)

4 protocols using potassium nitrosodisulfonate

1

Mitochondrial ROS Measurement Protocol

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All chemical used in this work were of analytical grade and purchased from Sigma (St. Louis, MO, USA), unless specified otherwise. MitoSOX Red (MitoHE) was purchased from Life Technologies (Carlsbad, CA, USA). Potassium nitrosodisulfonate was purchased as a 50–75 % pure powder (Sigma). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), L-glutamine, penicillin, and streptomycin were obtained from ATCC (Manassas, VA, USA). Polydimethylsiloxane (PDMS) microdevices were prepared from the Sylgard 184 Elastomer Kit (Ellsworth Adhesives, Germantown, WI, USA).
The pH values of solutions were adjusted using 1.5 M sodium hydroxide and 0.1 M hydrochloric acid solutions as needed.
de Campos R.P., Siegel J.M., Fresta C.G., Carusoab G., da Silva J.A, & Lunte S.M. (2015). Indirect detection of superoxide in RAW 264.7 macrophage cells using microchip electrophoresis coupled to laser-induced fluorescence. Analytical and bioanalytical chemistry, 407(23), 7003-7012.
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2

EPR Spectroscopy of Glycyl Radicals

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EPR spectra of the glycyl radical was collected at 80 K. Spectra were recorded at 9.37 GHz with a modulation amplitude of 3 G, microwave power of 1.26 μW, and a 100 kHz modulation frequency. A center field of 3350 G, a sweep time of 21 s, and a sweep width of 200 G were used. Each spectrum shown is an average of 10 scans. Potassium nitrosodisulfonate (Fremy’s salt, Sigma Aldrich) was used as a standard. The double integrals of each spectrum were calculated using Xenon software and compared to the double integrals obtained from Fremy’s standard to obtain concentrations of glycyl radical.
Andorfer M.C., King-Roberts D.T., Imrich C.N., Brotheridge B.G, & Drennan C.L. (2023). Development of an in vitro method for activation of X-succinate synthases for fumarate hydroalkylation. iScience, 26(6), 106902.
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3

Quantification of Deuterated Estradiol

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E2-2, 4, 16, 16, 17-d5 ([2H5]-E2) was from C/D/N Isotope (Canada H9R 1H1). Acetone, acetonitrile, ethyl acetate, and methyl alcohol were obtained from TEDIA (Charlotte, NC288224, USA). E2, chloroform, N-Acetyl-L-Cysteine (NAC), L-Ascorbic acid (99%), trifluoroacetic acid anhydride (TFAA), methanesulfonic acid (MSA), human serum Alb, human Hb, and potassium nitrosodisulfonate were purchased from Sigma-Aldrich Inc. (St Louis, MO63178, USA).
Lin C., Chen D.R., Kuo S.J., Feng C.Y., Chen D.R., Hsieh W.C, & Lin P.H. (2022). Profiling of Protein Adducts of Estrogen Quinones in 5-Year Survivors of Breast Cancer Without Recurrence. Cancer Control : Journal of the Moffitt Cancer Center, 29, 10732748221084196.
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4

Synthesis of 2-Hydroxyethidium from Hydroethidine

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Menadione, phosphate buffer (DPBS), dihydroethidium (DHE), thiazole orange (TO), diethylene triamine penta-acetic acid (DTPA), uridine 5'-diphospho-glucuronic acid (UDPGA) and potassium nitrosodisulfonate (NDS) were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). 1-benzyl-1,4-dihydronicotinamide (BNAH) was purchased from TCI Chemicals (Zwijndrecht, Belgique) and dimethylsulfoxyde (DMSO), FBS (Foetal Bovin serum), DMEM/F12, ammonium acetate, methanol, acetonitrile from ThermoFisher Scientific (Illkirch, France), DMPO (5,5-dimethyl-1-pyrroline N-oxide) from Dojindo (Kumamoto, Japon) and Trypsine from Corning (Boulogne-Billancourt, France). S29434 (N-[2-(2-methoxy-6H-dipyrido[2,3-a:3,2-e] pyrrolizin-11-yl)ethyl]-2-furamide) was provided by the Institut de Recherches Servier (Croissy-sur-Seine, France). 2-Hydroxyethidium (2OH-E+) was synthesized by the reaction of hydroethidine (DHE) with potassium nitrosodisulfonate (NDS) in the proportion 2/1 (Zielonka et al., 2008 (link)). The synthesis was performed by mixing: 200 µl of hydroethidine (20 mM) in 4 ml of phosphate (50 mM) DPTA buffer (100 µM), 24 ml of water, and 8 ml of NDS (1 mM) with gentle agitation. The mixture was kept at room temperature for 2 h, then purified by solid phase chromatography (SPE).
Chhour M., Perio P., Gayon R., Ternet-Fontebasso H., Ferry G., Nepveu F., Boutin J.A., Sudor J, & Reybier K. (2021). Association of NQO2 With UDP-Glucuronosyltransferases Reduces Menadione Toxicity in Neuroblastoma Cells. Frontiers in Pharmacology, 12, 660641.
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