Total rna kit

RNA was extracted from the collected peach fruit tissues using a Total RNA Kit (Sangon, Shanghai, China) according to the manufacturer’s instructions. RNA samples were evaluated in 1% agarose gels to determine the levels of degradation and contamination and then analyzed using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, USA) to determine RNA quantity and quality. First-strand cDNA was synthesized from the RNA samples with a Reverse Transcriptase kit (Tiangen, Beijing, China). The cDNA products were diluted to 20 ng µL⁻¹ for subsequent quantitative reverse-transcription PCR (qRT-PCR) analyses.

Total RNA was extracted from each tissue using the plant Total RNA Kit (Sangon Biotech, Shanghai, China), according to the manufacturer’s instructions. The concentration and purity of extracted RNA were measured by NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). Only the RNA absorbing ratio of 1.8–2.0 at OD260 nm/OD280 nm and 2.0–2.6 at OD260 nm/OD230 nm were used for subsequent analysis. In addition, the integrity of RNA was evaluated by 1% agarose gel electrophoresis.