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Ccr7 bv711

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CCR7-BV711 is a fluorochrome-conjugated antibody that binds to the CCR7 receptor, which is expressed on various immune cells and plays a role in lymphocyte trafficking and homing. This product can be used for flow cytometric analysis of CCR7 expression on cell populations.

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Lab products found in correlation

Cd8 bv570, by BioLegend (2 mentions) Cd16 apc, by BD (1 mentions) Cd56 fitc, by BD (1 mentions) Cd3 pc7, by BD (1 mentions) Nkp30 bv711, by BD (1 mentions) Cxcr3 bv421, by BD (1 mentions) Nkp44 percp efluor 710, by Thermo Fisher Scientific (1 mentions) Cxcr1 pe, by R&D Systems (1 mentions) Cd3 pe vio770, by Miltenyi Biotec (1 mentions) Tigit percp efluor710, by Thermo Fisher Scientific (1 mentions) Cd56 bv510, by BD (1 mentions) Cd16 buv737, by BD (1 mentions) Zombie nir fixable viability dye, by BioLegend (1 mentions) Nkg2d apc, by BD (1 mentions) Pd 1 bv650, by BD (1 mentions) Cd69 bv421, by BD (1 mentions) Cd69 apc, by BD (1 mentions) Cd14 bv650, by BioLegend (1 mentions) Tnf bv605, by BioLegend (1 mentions) Cd28 ecd, by Beckman Coulter (1 mentions)

3 protocols using ccr7 bv711

1

Comprehensive NK Cell Profiling in Melanoma

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NK cell phenotype of melanoma patients enrolled in the trial was examined using fluorochrome-conjugated antibodies against the following cell-surface markers: CD56-FITC, CD3-PC7, CD16-APC, CD69-BV421, NKp30-BV711, CXCR3-BV421, CCR3-BV510 (BD Biosciences; San Diego, CA), NKp44-PerCP eFluor 710 (eBioscience; San Diego, CA), CXCR1-PE (R&D Systems; Minneapolis, MN), CCR7-BV711 (BioLegend; San Diego, CA), and matching IgG isotype controls from the same vendors. The immune checkpoint and NK cell activation receptor panel included the following markers: Zombie NIR Fixable Viability Dye (BioLegend; San Diego, CA), CD3-PE-Vio770 (Miltenyi Biotec; San Diego, CA), ANK-1-PE (Santa Cruz Biotechnology; Dallas, TX), TIGIT-PerCP eFluor 710 (eBioscience), CD45-BUV395, CD56-BV510, CD16-BUV737, NKG2D-APC, NKp46-BV711, CD69-BV421, and PD-1-BV650 (BD Biosciences).
Vujanovic L., Chuckran C., Lin Y., Ding F., Sander C.A., Santos P.M., Lohr J., Mashadi-Hossein A., Warren S., White A., Huang A., Kirkwood J.M, & Butterfield L.H. (2019). CD56dim CD16− Natural Killer Cell Profiling in Melanoma Patients Receiving a Cancer Vaccine and Interferon-α. Frontiers in Immunology, 10, 14.
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2

Comprehensive Lymphocyte Immunophenotyping

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The following antibodies were used for phenotyping and functional evaluation of lymphocytes in this study: CD107a-BV421 (Clone H4A3, Biolegend), CD4-PECy5.5 (Clone S3.5, Invitrogen), CD8-BV570 (Clone RPA-T8, Biolegend), CD14-BV650 (Clone M5E2, Biolegend), CD20-BV650 (Clone 2H7, Biolegend), CD16-BV650 (Clone 3G8, Biolegend), CD28-ECD (Clone CD28.2, Beckman Coulter), CD95-PECy5 (Clone Dx2, BD Biosciences), CD38-PE (Clone OKT10, NIH NHP Reagent Resource, University of Massachusetts, Boston, MA), CCR7- BV711 (Clone G043H7, Biolegend). CD3-APCCy7 (Clone SP34-2, BD Biosciences), granzyme B-AF700 (Clone GB11, BD Biosciences), T-bet-PECy7 (Clone 4B10, eBioscience), Ki67-FITC (Clone B56, BD Bioscience) or Ki67-BV786 (Clone B56, BD Bioscience), CD69-APC (Clone FN50, BD Bioscience) or CD69-BV605 (Clone FN50, Biolegend), Perforin-FITC (Clone pf344, MabTech), TNF-BV605 (Clone MAb11, Biolegend), IFNg-BV785 (Clone 4S.B3, Biolegend), Live Dead Fixable Aqua Dead Cell Stain (Molecular Probes).
Roberts E.R., Carnathan D.G., Li H., Shaw G.M., Silvestri G, & Betts M.R. (2016). Collapse of Cytolytic Potential in SIV-Specific CD8+ T Cells Following Acute SIV Infection in Rhesus Macaques. PLoS Pathogens, 12(12), e1006135.
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3

Intracellular Cytokine Staining Workflow

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Intracellular cytokine staining was performed as previously described(1) using the following markers: CD3-APCH7, CD107a PECy7, CD14-Pacific Blue, CD16-Pacific Blue, CD4-PECy5.5, IFN-γ-PerCPCy5.5, CD45RO-AF700, CD19-Pacific Blue (BD Biosciences, San Jose, CA), TNFα-AF647, CD8-BV570, CCR7-BV711 (BioLegend, San Diego, CA), granzyme B-PE Texas Red (Invitrogen), CD27-PECy5 (eBioscience, San Diego, CA) perforin-FITC(Abcam, Cambridge, UK). Prepared cells were acquired using an LSR II flow cytometer equipped with BD FACSDiva software (BD Biosciences). Acquired data was analyzed using the FlowJo software version 7.6.3 (Tree Star).
Morrow M.P., Kraynyak K.A., Sylvester A.J., Shen X., Amante D., Sakata L., Parker L., Yan J., Boyer J., Roh C., Humeau L., Khan A.S., Broderick K., Marcozzi-Pierce K., Giffear M., Lee J., Trimble C.L., Kim J.J., Sardesai N.Y., Weiner D.B, & Bagarazzi M.L. (2016). Augmentation of cellular and humoral immune responses to HPV16 and HPV18 E6 and E7 antigens by VGX-3100. Molecular Therapy Oncolytics, 3, 16025-.
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