Ethanol (analytical pure grade) was obtained from Zhen Xin Co. Ltd. (Shanghai, China). Isoflurane was purchased from Baxter Healthcare Co. (Deerfield, IL, USA). Rat CORT ELISA kits were obtained from Assay pro LLC. (Saint Charles, MO, USA). Rat IGF-1 ELISA kits were purchased from RD Systems, Inc. (Minneapolis, MN, USA). TRIzol reagent kits were obtained from Omega Bio-Tek (Doraville, USA). GeXP multiplex gene expression analysis kits were purchased from Beckman-Coulter Inc. (Fullerton, CA, USA). The oligonucleotide primers for rat Q-PCR (PAGE purification) and GeXP multiplex gene expression analysis (HPLC purification) were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). Polyclonal rat IGF-1, IRS1, AKT1/2, SOX9 and Col2a1 antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Other chemicals and agents were of analytical grade.
Rat igf 1 elisa kits
Manufactured by R&D Systems
Sourced in United States
The Rat IGF-1 ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure the concentration of Rat Insulin-like Growth Factor 1 (IGF-1) in serum, plasma, and cell culture supernatants. The kit utilizes a specific antibody coated on a microplate to capture IGF-1 from the samples, and a detection antibody conjugated to a reporter enzyme for quantification.
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2 protocols using rat igf 1 elisa kits
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Detailed Reagent Sourcing for Biochemical Analysis
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Reagents and Materials for Rat CORT and IGF-1 Assays
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Isoflurane was purchased from Baxter Healthcare Co. Rat corticosterone (CORT) ELISA kits were obtained from Assaypro LLC. Rat IGF-1 ELISA kits were purchased from RD Systems Inc. Total cholesterol (TCH), LDL-cholesterol and HDL-cholesterol assay kits were purchased from Sangon Biotech Co. Ltd. Reverse transcription kits were purchased from TaKaRa Biotechnology Co. Ltd. GeXP multiplex gene expression analysis kits were purchased from Beckman Coulter Inc. The oligonucleotide primers for rat GeXP multiplex gene expression analysis (HPLC purification) were synthesised by Sangon Biotech Co. Ltd. Polyclonal antibodies for Col2a1, IGF-1, LXR and ABCA1 were obtained from Santa Cruz Biotechnology Inc. Other chemicals and agents used were of analytical grade.
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