1H NMR spectra (500 mHz superconducting Fourier transform (FT)-NMR spectrometer, Varian Unity Inova 500 MHz NB High-Resolution FT NMR; Varian Inc., Santa Clara, CA) was used to monitor the synthesis of conjugates. To measure the 1H NMR spectra, conjugates were dissolved in D2O, DMSO or D2O/DMSO mixtures.
Varian unity inova 500 mhz nb high resolution ft nmr
Manufactured by Agilent Technologies
Sourced in United States
The Varian Unity Inova 500 MHz NB High-Resolution FT NMR is a nuclear magnetic resonance (NMR) spectrometer designed for high-resolution analysis. It operates at a frequency of 500 MHz and is capable of performing Fourier transform NMR (FT-NMR) measurements.
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4 protocols using varian unity inova 500 mhz nb high resolution ft nmr
1
1H NMR Analysis of Conjugates
2
Characterization of ChitoPEG/DOX/IO Nanocomposites
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1H NMR spectra (500 mHz superconducting Fourier transform (FT)-NMR spectrometer, Varian Unity Inova 500 MHz NB High-Resolution FT NMR; Varian Inc., Santa Clara, CA, USA) was used to monitor the synthesis of conjugates. To measure the 1H NMR spectra, conjugates were dissolved in D2O, DMSO, or D2O/DMSO mixtures.
A transmission electron microscope (TEM) (H-7600, Hitachi Instruments Ltd., Tokyo, Japan) was used to observe the morphology of ChitoPEG/DOX/IO nanocomposites. ChitoPEG/DOX/IO nanocomposites in water (nanoparticle weight 1 mg/mL) were dropped onto a carbon film-coated copper grid and dried at room temperature overnight. TEM observation was carried out at 80 kV.
Particle size of ChitoPEG/DOX/IO nanocomposites was analyzed with Nano-ZS at room temperature (Malvern, Worcestershire, UK) (nanoparticle content in water was less than 0.1 wt.%).
A transmission electron microscope (TEM) (H-7600, Hitachi Instruments Ltd., Tokyo, Japan) was used to observe the morphology of ChitoPEG/DOX/IO nanocomposites. ChitoPEG/DOX/IO nanocomposites in water (nanoparticle weight 1 mg/mL) were dropped onto a carbon film-coated copper grid and dried at room temperature overnight. TEM observation was carried out at 80 kV.
Particle size of ChitoPEG/DOX/IO nanocomposites was analyzed with Nano-ZS at room temperature (Malvern, Worcestershire, UK) (nanoparticle content in water was less than 0.1 wt.%).
3
NMR Characterization of Conjugates
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500 mHz super conducting Fourier transform (FT)-NMR spectrometer (Varian Unity Inova 500 MHz NB High Resolution FT NMR; Varian Inc., Santa Clara, CA, USA) was used to measure 1H NMR spectra of conjugates. Chemicals were dissolved in DMSO or D2O/DMSO mixtures to measure 1H NMR spectra.
4
NMR Spectroscopic Analysis of Chemical Synthesis
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Chemical composition and synthesis procedures were monitored using 1H NMR spectra (500 mHz superconducting Fourier transform (FT)-NMR spectrometer, Varian Unity Inova 500 MHz NB High-Resolution FT NMR; Varian Inc., Santa Clara, CA, USA). Each of the chemicals in the synthesis procedures were dissolved in DMSO or D2O/DMSO mixtures and then analyzed.
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