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Progesterone

Manufactured by Siemens
Sourced in United States

Progesterone is a clinical laboratory equipment designed for the quantitative determination of progesterone levels in human serum or plasma samples. It is a hormone that plays a crucial role in the menstrual cycle, pregnancy, and other physiological processes.

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4 protocols using progesterone

1

Hormonal Biomarkers of Menstrual Cycle

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At each visit, blood samples were collected and frozen (−70˚C) before analysis with standard immunoassay kits for follicle-stimulating hormone (FSH, IU.l−1) (Siemens Healthcare Diagnostics, Los Angeles, CA, USA; intraassay and interassay coefficients of variations were 2.6% and 5.5%, respectively; sensitivity, 0.1 IU.l−1), progesterone (ng.ml−1) (Siemens Medical Solutions Diagnostics, Los Angeles, CA, USA; intraassay and interassay coefficients of variations were 4.0% and 5.7%, respectively; sensitivity, 0.2 ng.ml−1) and estradiol (pg.mol−1) (Beckman Coulter Inc., Fullerton, CA, USA; intraassay and interassay coefficients of variations were 6.7% and 7.6%, respectively; sensitivity, 3 pg.mol−1). progesterone levels ≥3 ng.ml−1 were used as a cut-off to classify the luteal phase46 (link) (five women with and five women without insomnia had a PSG night recorded in the luteal phase of their menstrual cycles; in all the remaining nights progesterone was <3 ng.ml−1).
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2

Hormonal Profile Assessment

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Serum levels of estrogen, progesterone, testosterone, prolactin, follicle-stimulating hormone, and luteinizing hormone (Siemens, San Francisco, California, USA) were determined by chemiluminescence. An electrochemical luminescence analysis system (Roche, CobasE-601; Roche, Basel, Switzerland) was used for detection. The test was always performed by the same inspector. All tested items met the quality control requirements before proceeding to the analysis of the specimens. The internal coefficient of variation was 3.8–5.4% and between batch 4.3–4.6%.
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3

Serum Hormone and Adipokine Measurements

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Serum LH and FSH levels were measured using a radioimmunoassay (BioSource SA, Nivelles, Belgium). Serum estradiol level was measured using a chemiluminescence immunoassay kit (Estradiol II, Roche Diagnostics, Indianapolis, IN, USA) and a Roche Analytics E-170 Immunology Analyzer (Diamond Diagnostics, Holliston, MA, USA). Progesterone level was measured using a radioimmunoassay kit (Progesterone, Siemens Healthcare Diagnostics, East Walpole, MA, USA) and an ADVIA Centaur CP Immunoassay System (Siemens, Seoul, Korea). Serum leptin level was measured using the human leptin “Dual Range” enzyme-linked immunosorbent assay (ELISA) kit (EZHL-80SK, Merck Millipore Co., Darmstadt, Germany) with a sensitivity of 0.5 ng/mL, an interassay coefficient of variation (CV) of 2.6%–6.2%, and an intra-assay CV of 2.6%–4.6%. Finally, adiponectin level was measured using the human adiponectin ELISA kit (EZHADP-61K, Merck Millipore Co.) with a sensitivity of 1.5 ng/mL, an interassay CV of 2.4%–8.4%, and intra-assay CV of 1.0%–7.4%, on a SpectraMax 190 Microplate Spectrophotometer (Molecular Devices, Sunnyvale, CA, USA).
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4

Pregnancy Hormone Biomarker Assay Protocol

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Serum samples were shipped to and analyzed at the Central Ligand Assay Satellite Services laboratory in the Department of Epidemiology at the University of Michigan, School of Public Health (see Supplemental Material for quality control and assurance details). Progesterone (Siemens, catalog no. 1586287) (ADVIA ), sex hormone-binding globulin [SHBG] (Siemens, catalog no. 6520781) (ADVIA ), testosterone (Siemens, catalog no. 5476206) (ADVIA ), total triiodothyronine [T3] (Siemens, catalog no. 8427516) (ADVIA) total thyroxine [T4] (Siemens, catalog no. 9236439) (ADVIA ), free T4 (fT4; Siemens, catalog no. 6490106) (ADVIA ), and TSH (Siemens, catalog no. 8700387) (ADVIA ) were measured using chemiluminescence immunoassays. Estriol [E3] (DiaMetra, catalog no. DKO019) (DiaMetra ) and corticotropin-releasing hormone [CRH] (LifeSpan, catalog no. LS-F5352) (LifeSpan ) were measured using enzyme immunoassays. The ratios of Progesterone/E3 and T3/T4 were calculated and assessed as these ratios may be better indicators of adverse pregnancy outcomes than each of the individual hormone measurements (Dietrich et al. 2012 (link); Romero et al. 1988 (link)). Overall, 1070 women had hormone measurements, from those 597 and 473 had one and two measurements, respectively.
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