Prior to freezing and lyophilizing, as well as post, particle size and zeta potential were analyzed via a Malvern Zetasizer Nano ZS. Briefly, a small amount of lyophilized NPs was re-suspended in 1 mL DDI H2O, vortexed for 30 seconds, then transferred to a disposable cuvette (Sarstedt) for analysis of particle size (in nm) by dynamic light scattering. The sample was then transferred to a disposable capillary cell (Malvern) for analysis of zeta potential. For each NP batch, the mean diameter ± S.D. for three measurements was determined. The polydispersity index (PDI) was also quantified to establish particle size distribution.
Disposable cuvette
Manufactured by Sarstedt
Sourced in Germany
Disposable cuvette is a single-use container used in spectrophotometric analysis. It is designed to hold samples for measurement of light absorption or transmission properties.
Automatically generated - may contain errors
3 protocols using disposable cuvette
1
Nanoparticle Characterization via Zetasizer
2
Nanoparticle Characterization via Zetasizer
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Prior to freezing and lyophilizing, as well as post, particle size and zeta potential were analyzed via a Malvern Zetasizer Nano ZS. Briefly, a small amount of lyophilized NPs was re-suspended in 1 mL DDI H2O, vortexed for 30 seconds, then transferred to a disposable cuvette (Sarstedt) for analysis of particle size (in nm) by dynamic light scattering. The sample was then transferred to a disposable capillary cell (Malvern) for analysis of zeta potential. For each NP batch, the mean diameter ± S.D. for three measurements was determined. The polydispersity index (PDI) was also quantified to establish particle size distribution.
3
Dynamic Light Scattering of NTS-Polyplex Nanoparticles
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The Litesizer DLS 500 (Anton Paar, Graz, Austria) was used to measure the size and charge of NTS-polyplex NPs harboring either the pTracer-rNurr1-V5 or the pEGFP-N1 prepared in DMEM without phenol red. First, the diameter distribution was determined by dynamic light scattering in 1 mL of NTS-polyplex NPs at RT (25°C, angle 90°) using a disposable cuvette (Sarstedt, Nümbrecht, Germany). Then, the zeta potential was measured in 50 μL of NTS-polyplex NPs using a Univette Low Volume and 4 V. The dataset was processed using the KalliopeTM Software (Anton Paar).
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