DMEM-F12 medium, phosphate buffered solution (PBS) fetal bovine serum (FBS), DCFH-DA probe and mitochondria-targeted MitoSOX probe (M36008) were purchased from Invitrogen. Hcy, ATX, MTT, propidium iodide (PI) were obtained from Sigma. TUNEL-DAPI kit, Mito-SOX probe and BCA assay kit were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Primary antibodies, including cleaved PARP (#94885), active-caspase-3 (#9664), active-caspase-7 (#8438), active-caspase-9 (#9507), Bax (#2772), Bad (#9292), Bcl-xL (#2764), Ser428-ATR (#2853), Ser15-p53 (#9284), total-p53 (#2524), and Ser139-histone (#9718) were all obtained from Cell Signaling Technology (Beverly, USA). Bcl-2 (#14-6992-82), CD-34 (#MA1-10202) and Ser-1981-ATM (#14-9046-82) were bought from Invitrogen (Carlsbad, USA). All solvents used were of high performance liquid chromatography (HPLC) grade.
Mito sox probe
Manufactured by Beyotime
Sourced in China
The Mito-SOX probe is a fluorogenic dye that is specifically targeted to mitochondria. It is used to detect superoxide (O2-) in the mitochondria of live cells.
Automatically generated - may contain errors
Lab products found in correlation
Tunel dapi kit, by Beyotime (4 mentions)
Bca assay kit, by Beyotime (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Dcfh da probe, by Thermo Fisher Scientific (2 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)
Propidium iodide, by Merck Group (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Active caspase 7, by Cell Signaling Technology (1 mentions)
Active caspase 9, by Cell Signaling Technology (1 mentions)
Ser15 p53, by Cell Signaling Technology (1 mentions)
Total p53, by Cell Signaling Technology (1 mentions)
Active caspase 3, by Cell Signaling Technology (1 mentions)
Bcl 2, by Thermo Fisher Scientific (1 mentions)
Cleaved parp, by Cell Signaling Technology (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Infinite m200 pro plate reader, by Tecan (1 mentions)
5 protocols using mito sox probe
1
Oxidative Stress Evaluation in Cells
2
Oxidative Stress Evaluation Protocol
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DMEM-F12 medium, phosphate buffered solution (PBS), foetal bovine serum (FBS), and DCFH-DA probe were all purchased from Invitrogen. MTT, propidium iodide (PI) and other agents were obtained from Sigma. TUNEL-DAPI kit, Mito-SOX probe and BCA assay kit were purchased from Beyotime Institute of Biotechnology (Shanghai, China). All primary antibodies were obtained from Cell Signalling Technology (Beverly, USA). All solvents used were of high-performance liquid chromatography (HPLC) grade.
3
Mitochondrial ROS Detection Using mitoSOX
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The mitoSOX probe (Beyotime Institute of Biotechnology) was used to determine mtROS production. First, cells were seeded onto 6-well cell culture plates. After 24 h of incubation, G-Rh2 specificity was determined by pretreating HeLa cells with mitoQ (5 µM) for 1 h prior to treatment with G-Rh2 (35 and 45 µM) for 24 h. The cells were collected by centrifugation and washed with cold PBS prior to adding 5 µM mitoSOX probe (cat. no. M36008; Invitrogen; Thermo Fisher Scientific, Inc.) and incubating the mixture at 37°C for 20 min in the dark. The cells were then washed twice with PBS, 200 µl cell suspension (1×106 cells/ml) was added to the 96-well black plate and fluorescence units were detected with an Infinite M200 PRO plate reader (Tecan Group, Ltd.) at 510/580 nm excitation/emission wavelengths.
4
Comprehensive Cell Viability Assays
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TUNEL-DAPI kit, JC-1 pobe, DCFH-DA probe, Mito-SOX probe, PI staining solution and MTT were bought from Beyotime company (Shanghai, China). DMEM medium, fetal bovine serum (FBS), and penicillin–streptomycin were purchased from Invitrogen (Carlsbad, CA, United States). All antibodies and inhibitors used in the present study were purchased from Cell Signaling Technology (Beverly, MA, United States). Water used in this study was supplemented by Milli-Q system from Millipore.
5
Oxidative Stress and Cell Viability Assay
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NB was obtained from BenFu Technology Co., Ltd. (Guangzhou, China) DMEM, PBS, FBS, 2′.7′-dichlorofluorescin diacetate (DCFH-DA) probe and mitochondria-targeted MitoSOX probe (M36008) were all purchased from Thermo Fisher Scientific (Waltham, MA, USA). Temozolomide (TMZ), MTT and propidium iodide (PI) were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). TUNEL-DAPI kit, MitoSOX probe and BCA assay kit were purchased from Beyotime Institute of Biotechnology (Shanghai, China). All antibodies used in this study were obtained from Cell Signaling Technology (Beverly, MA, USA). All solvents used were of high performance liquid chromatography (HPLC) grade.
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