Rt random primers high capacity cdna reverse transcriptase kit

The Trizol LS^® reagent (Invitrogen Canada Inc., Burlington, ON, Canada) and the Trizol reagent (Invitrogen Canada Inc., Burlington, ON, Canada) were used in accordance with the manufacturer’s instructions to extract RNA from swab and tissue samples, respectively. Then, the amount of extracted RNA was quantified at 260 nm wavelength with a Nanodrop 1000 spectrophotometer (ThermoFisher Scientific, Wilmington, DE, USA). Complementary DNA (cDNA) was synthesized using 1000 ng of RNA from swab samples and 2000 ng of RNA from tissue samples with the RT random primers high-capacity cDNA reverse transcriptase kit (Invitrogen Life Technologies, Carlsbad, CA, USA) following the manufacturer’s guidelines.

The OP and CL swab samples were vortexed and 250 μL of the sample was used to extract the RNA using Trizol LS^® reagent (Invitrogen Canada Inc., Burlington, ON, Canada) and total RNA was extracted from tissue samples using Trizol reagent (Invitrogen Canada Inc., Burlington, ON, Canada) according to manufacturer’s guidelines. Following this, the amount of extracted RNA was quantified using a Nanodrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA.) at 260 nm wavelength. Finally, 1000 ng of RNA from swab samples and 2000 ng of RNA from tissue samples were used to synthesize cDNA using RT random primers high-capacity cDNA reverse transcriptase kit (Invitrogen Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions.