Recombinant murine cxcl1

Manufactured by Thermo Fisher Scientific

Sourced in United States

Recombinant murine CXCL1 is a chemokine protein produced in E. coli. It functions as a chemoattractant, promoting the migration of cells expressing the CXCR2 receptor.

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Lab products found in correlation

Recombinant murine ccl2, by Thermo Fisher Scientific (2 mentions) Transwell polycarbonate permeable supports, by Corning (1 mentions) Normal goat igg control, by R&D Systems (1 mentions) Tisseel, by Baxter (1 mentions)

4 protocols using recombinant murine cxcl1

MDSC Migration Assay with CXCL1 and CXCR2 Antagonist

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In vitro migration of murine MDSCs was evaluated in 24-well plates with transwell polycarbonate-permeable supports (8.0 μm; Costar Corning, Cambridge, MA, USA). MDSCs (1 × 10⁶/mL) were seeded in the upper chambers of the inserts 30 min after incubation with culture supernatants of the differential expression Hepa1-6 cells at each concentration. Next recombinant murine CXCL1 (PeproTech, Rocky Hill, NJ, USA) were placed in the lower chamber at a concentration of 0, 10, or 100 ng/mL. The number of MDSCs in the bottom compartment was counted 24 h later.
To evaluate the suppressed migration of MDSCs, MDSCs (1 × 10⁶/mL) were plated in the upper chambers of the inserts 30 min after incubation with the CXCR2 antagonist at a concentration of 0, 100, or 1000 ng/mL at each concentration, the number of MDSCs in the bottom compartment was counted 24 h later. Next recombinant murine CXCL1 (PeproTech, Rocky Hill, NJ, USA) was placed in the lower chamber at a concentration of 10 ng/ml. After incubation for 24 h, the number of MDSCs in the bottom compartment was counted.

Xia S., Wu J., Zhou W., Zhang M., Zhao K., Liu J., Tian D, & Liao J. (2021). SLC7A2 deficiency promotes hepatocellular carcinoma progression by enhancing recruitment of myeloid-derived suppressors cells. Cell Death & Disease, 12(6), 570.

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Neutralizing IL-1α and Restoring CXCL1 in Aspergillosis

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For IL-1α neutralization studies, normal goat IgG control and anti-mIL-1α neutralizing antibody were purchased from R&D systems. IgG control or anti-mIL-1α neutralizing antibody were administered i.p. at 40 μg per mouse. Administration of neutralizing antibody was given every other day, beginning the day prior to A. fumigatus challenge. For CXCL1 reconstitution studies, recombinant murine CXCL1 was purchased from PeproTech. CXCL1 was administered i.t. at 0.1–0.5 μg per mouse and was given 3 hours after A. fumigatus challenge. For the hIL1ra studies, recombinant hIL1ra and the appropriate placebo (Amgen) were kindly provided by Dr. Charles A. Dinarello. The hIL1ra and placebo were administered i.p. at 200 mg per mouse given at −24, 0, and +24 h relative to A. fumigatus challenge.

Caffrey A.K., Lehmann M.M., Zickovich J.M., Espinosa V., Shepardson K.M., Watschke C.P., Hilmer K.M., Thammahong A., Barker B.M., Rivera A., Cramer R.A, & Obar J.J. (2015). IL-1α Signaling Is Critical for Leukocyte Recruitment after Pulmonary Aspergillus fumigatus Challenge. PLoS Pathogens, 11(1), e1004625.

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Chemokine-Loaded Fibrin Sealant for Wound Healing

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Fibrin sealant (Tisseel, Baxter) was delivered to the wound bed by co-application of thrombin and fibrinogen, which were prepared under sterile conditions according to the manufacturer instructions. The sealer protein protease inhibitor was omitted from the mixture to facilitate delivery of the recombinant chemokines to the wound bed via fibrin degradation. Directly before application, recombinant murine CCL2 (Peprotech) and recombinant murine CXCL1 (Peprotech) were mixed into the fibrinogen component. Control mice were treated with Tisseel without chemokines. The fibrinogen and thrombin components were maintained at 37°C to avoid polymerization. Using two pipets, equal volumes of fibrinogen and thrombin were simultaneously applied to the wound beds of anesthetized mice and allowed to polymerize. Treatments were given every day from wound days 1 to 7, then every other day for the remainder of the experiment. Each chemokine treatment contained 10ng of recombinant CCL2 and 10ng of recombinant CXCL1.
Application volumes were adjusted according to wound bed area and ranged from 30uL to 10uL.

Crane M.J., Xu Y., Monaghan S.F., Hall B.M., Albina J.E., Henry W.P., Tran H.L., Chhabria K., Jordon A.R.D., Carlsen L., & Jamieson A.M. (2020). Pulmonary infection interrupts acute cutaneous wound healing through disruption of chemokine signals.

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Chemokine-Induced Sponge Angiogenesis

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Recombinant murine CCL2 (Peprotech) and recombinant murine CXCL1 (Peprotech) were diluted in 1x PBS for injection into implanted PVA sponges. The backs of mice were cleaned with iodine solution and isopropyl alcohol. 0.5ug of each chemokine mixed in a total volume of 50uL of PBS was injected through the skin and into the center of each sponge for a total treatment of 3ug per wound. Control mice received injections of PBS vehicle. Mice were injected on wound days 5 and 6, and sponges were isolated on wound day 7.

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