Anti-mouse CD140b (catalog 136002, RRID: AB_1953332), APC anti-mouse CD140b antibody (catalog 136008, RRID: AB_2268091), Alexa Fluor 647 anti-human CD31 antibody (catalog 303111, RRID:AB_493077), Alexa Fluor 488 anti-human CD31 antibody (catalog 303109, RRID:AB_493075), APC anti-human CD140b (PDGFRβ) antibody (catalog 323608, RRID:AB_2162787), Brilliant Violet 421 anti-mouse CD45 antibody (catalog 103133, RRID:AB_10001045), BV421 anti-human CD117 (C-kit) antibody (catalog 313215, RRID:AB_10896056), BV421 anti-mouse CD117 (catalog 560557, RRID:AB_1645258), PE/Cy5 anti-human CD45 (catalog 304010, RRID:AB_314398), APC anti-mouse FCεRIα (catalog 134316, RRID:AB_10640121), Brilliant Violet 711 anti-human TNF-α antibody (catalog 502940, RRID AB_2563885), and APC anti-mouse TER-119/erythroid cells antibody (catalog 116212 RRID AB_313713) were from BioLegend. Mouse/rat CD31/PECAM-1 antibody (catalog AF3628, RRID:AB_2161028), mouse mast cell protease-6/Mcpt6 antibody (catalog MAB3736, RRID:AB_2240825), and BV510 anti-human FCεRIα (catalog 334626, RRID:AB_2564291) were from R&D Systems
Pe cy5 anti human cd45
Manufactured by BioLegend
PE/Cy5 anti-human CD45 is a monoclonal antibody that binds to the CD45 antigen expressed on human leukocytes. It is conjugated with a combination of phycoerythrin (PE) and cyanine 5 (Cy5) fluorescent dyes.
Automatically generated - may contain errors
Lab products found in correlation
Apc anti mouse fcεriα, by BioLegend (1 mentions)
Apc anti mouse cd140b antibody, by BioLegend (1 mentions)
Alexa fluor 647 anti human cd31 antibody, by BioLegend (1 mentions)
Alexa fluor 488 anti human cd31 antibody, by BioLegend (1 mentions)
Facscanto instrument, by BD (1 mentions)
Countbright beads, by Thermo Fisher Scientific (1 mentions)
Human fc block, by BD (1 mentions)
2 protocols using pe cy5 anti human cd45
1
Multicolor Flow Cytometry Analysis of Mouse and Human Cells
2
Flow Cytometric Analysis of Nasal Cells
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Cells obtained as described above from the nasal lavage fluid and the nasal swab were combined and subsequently counted. Cells were incubated with Human BD Fc block (BD Biosciences) for 10 min, followed by 0.045 µg PE‐Cy5 anti‐human CD45 (Leukocyte common antigen; clone HI30, Biolegend) and 0.1875 µg PE anti‐human CD326 (epithelial cell surface antigen; clone C4, Biolegend) in a 100 µl volume. Cells were incubated on ice for 20 min in the dark, followed by two washes with PBS stain buffer. Quantification of cell number counted was determined by CountBright beads (Thermo Fisher). Cells were fixed with 1% formaldehyde in PBS and analyzed on a BD Biosciences FACS Canto instrument within 3 days. FlowJo was used for data analysis.
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