Srebp 1

OA (HPLC $\ge$ 98 $\%$ ) was supplied from Yuanye (Shanghai, China) and dissolved by 2 $\%$ Tween 80 phosphate-buffered saline (PBS pH:7.2-7.4). Orlistat capsules were purchased from Aili (Chongqing, China). The kits of TC, TG, HDL-C, LDL-C, ALT, AST, GSH, and MDA were obtained from Nanjing Jiancheng Biotechnology Co. (Nanjing, Jiangsu, China). The enzyme-linked immunosorbent assay (ELISA) kits of interleukin 6 (IL-6), tumor necrosis factor- $\alpha$ (TNF- $\alpha$ ), Glucagon-Like Peptide 1 (GLP-1) and Monocyte Chemotactic Protein 1 (MCP-1) was supplied from Meimian (Yancheng, Jiangsu, China). The primary antibodies of SREBP-1, TGR5, UCP-1, and $\beta$ -actin were purchased from Abclonal (Wuhan, Hubei, China). Meanwhile, Abcam (Cambridge, UK) provides the secondary antibodies and PPAR $\gamma$ antibody.

Total protein from the cell was collected using the ProteinExt^®Mammalian Total Protein Extraction Kit (TransGen Biotech, Beijing, China), following the manufacturer’s protocol. The concentration of the protein was measured using the Bradford protein assay kit (Novoprotein, Shanghai, China). The protein was resolved on 10% SDS-PAGE and then transferred to a PVDF membrane, followed by sealing of the sealing fluid. The membranes were incubated with the corresponding primary antibodies for 8 h at 4 °C and subsequently incubated with the secondary anti-bodies (Goat Anti-Rabbit IgG H&L (HRP), Zen bioscience, Chengdu, China) for 2 h. Then the bands were washed three times, and the hypersensitive chromogenic solution was developed using chemiluminescence in Touch Imager Pro, e-BLOT Life Science (Shanghai) Co., Ltd. (Shanghai, China). The primary antibodies PCNA, CDK2, and CDK4 were purchased from Zen bioscience (Chengdu, China). The primary antibodies PPARγ, CEBP/α, FABP4, SREBP1, and RAD51 were purchased from the Abclonal (Wuhan, China).