Protease inhibitor cocktail (P8340), phosphatase inhibitor cocktail 2 (P5726) and 3 (P0044), and dimethyl sulfoxide (D8418) were purchased from Sigma-Aldrich. The primary antibodies used in this study from Santa Cruz biotechnology were cyclin D1 (sc-753); cyclin A (sc-596); cyclin B1 (sc-245). Additionally, we used pHH3ser10 (9701A; Cell signaling), LC-3 (L8918; Sigma), cleaved caspase-3, and PARP (cell signaling) and GAPDH (IMG-5019A-2; IMGENEX). Horseradish peroxidase-conjugated secondary antibodies of goat anti-rabbit (4010-05) and goat anti-mouse (1012-05) were purchased from Southern Biotech.
Dimethyl sulfoxide d8418
Manufactured by Merck Group
Sourced in United States
Dimethyl sulfoxide (D8418) is a clear, colorless liquid that is widely used as a solvent in various applications. It has a high boiling point and is miscible with water and many organic solvents. Dimethyl sulfoxide is a versatile chemical compound with a range of uses, including as a reaction medium, cryoprotectant, and penetration enhancer.
Automatically generated - may contain errors
Lab products found in correlation
Cyclin d1 sc 753, by Santa Cruz Biotechnology (1 mentions)
Cyclin a sc 596, by Santa Cruz Biotechnology (1 mentions)
Gapdh img 5019a 2, by Novus Biologicals (1 mentions)
Horseradish peroxidase conjugated secondary antibodies of goat anti rabbit 4010 05, by Southern Biotech (1 mentions)
Goat anti mouse 1012 05, by Southern Biotech (1 mentions)
Cyclin b1 sc 245, by Santa Cruz Biotechnology (1 mentions)
Lc3 l8918, by Merck Group (1 mentions)
αsma a2547, by Merck Group (1 mentions)
Smad2 3 3102, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit g 21234, by Thermo Fisher Scientific (1 mentions)
Phospho smad3 9520, by Cell Signaling Technology (1 mentions)
β actin a5441, by Merck Group (1 mentions)
Fibronectin 610077, by BD (1 mentions)
Tgf β 240 b, by R&D Systems (1 mentions)
Cip p4978, by Merck Group (1 mentions)
Ndufa9 459100, by Thermo Fisher Scientific (1 mentions)
Pa5 29975, by Thermo Fisher Scientific (1 mentions)
Cycloheximide 01810, by Merck Group (1 mentions)
Pgc1α h 300, by Santa Cruz Biotechnology (1 mentions)
Gst b 14, by Santa Cruz Biotechnology (1 mentions)
5 protocols using dimethyl sulfoxide d8418
1
Cell Cycle and Apoptosis Pathway Analysis
2
Fibrosis-related Protein Detection Assay
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PAI-1 (614024) and fibronectin (610077) antibodies were obtained from BD Biosciences (Franklin Lakes, NJ, USA). Phospho-Smad3 (#9520) and Smad2/3 (#3102) antibodies were provided by Cell Signaling (Danvers, MA, USA). CTGF antibodies (SC-101586) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), and TGF-β1 antibody (NBP1-80289) was obtained from Novus Biologicals (Littleton, CO). Horseradish peroxidase-conjugated goat anti-rabbit (G21234) and anti-mouse (G21040) antibodies were purchased from Invitrogen (Carlsbad, CA, USA). α-SMA (A2547) and β-actin (A5441) antibodies, MTT (M2128), and dimethylsulfoxide (D8418) were acquired from Sigma Chemicals (St. Louis, MO, USA). TGF-β (240-B) was purchased from R&D Systems (Minneapolis, MN, USA). CTGF antibody (sc-101586) obtained from Santa Cruz Biotechnology (Santa Cruz, CA). CCl4 (33650-0330) was received from Junsei Chemical (Kyoto, CO).
3
Cellular Protein Quantification and Validation
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Cycloheximide (01810) and calf-intestinal alkaline phosphatase (CIP, P4978) were purchased from Sigma. Dimethyl sulfoxide (D8418, Sigma-Aldrich, St Louis, MO, USA) was added to the cultures at 0.1% (v/v) as a solvent control. Specific antibodies against ATP5C1 (PA5-29975) and NDUFA9 (459100) were purchased from Invitrogen. Antibodies specific for ATP5A1 (51), SIRT3 (14.45), VDAC (B-6), SDHA (B-1), UQCRC2 (G-10), COX IV (D-20), PGC-1α (H-300), PGC-1β (E-9), NRF1 (H-285), NRF2 (C-20), Tfam (H-203), SIRT1 (H-300), PKCα (C-20), actin (I-19), V5 (H-9), Flag (D-8), His (AD1.1.10) and GST (B-14) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
4
Autophagy Regulation via AMPK Signaling
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3-MA (M9281), 4,6-diamidino-2-phenylindole dihydrochloride (DAPI, D9542), LC3 (L8918), dimethyl sulfoxide (D8418), and N’-(7-chloroquinolin-4-yl)-N,N-diethylpentane-1,4-diamine (chloroquine; C6628) were purchased from Sigma. Bafilomycin A1 (196000) was purchased from Calbiochem (San Diego, CA, USA). Alexa 488-conjugated anti-rabbit IgG (A17041) was purchased from Molecular Probes (Eugene, OR, USA). Actin (sc-1616) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-phospho-ACC (3661) and anti-phospho-AMPKα (2535) Abs were purchased from Cell Signaling Technology (Beverly, MA, USA).
5
Preparation of BTKi-1 Stock Solution
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Stock solution (100 mM) of BTKi-1 (Merck KGaA, Darmstadt, Germany) was obtained by dissolving 200 mg in 4.49 mL of DMSO (Dimethyl sulfoxide D8418-50 ml Sigma-Aldrich). Further dilutions were either in culture medium for mouse cerebellar slices or water for Xenopus experiments.
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