Mac 2 rat anti mouse antibody clone 3 38

For immunohistochemistry staining of paraffin-embedded tissue sections, Elite ABC kits and diaminobenzidine (DAB; AXXORA, Lörrach, Germany) were used. Cell density was described as cells per square millimeter, as previously reported [6 (link),7 (link)]. For mouse-derived antibodies, a mouse-on-mouse immunodetection kit (AXXORA) was used. On paraffin-embedded slides, the following primary antibodies were used: macrophages, MAC-2 rat anti-mouse antibody (clone 3/38; Cedarlane, ON, Canada); myofibroblasts, α-smooth muscle actin (αSMAC) mouse anti-mouse monoclonal antibody (clone 1A4; Sigma, Taufkirchen, Germany) [7 (link),8 (link)]. For detection of apoptotic cardiomyocytes, cleaved ASP 175 Caspase-3 rabbit anti-mouse polyclonal antibody (Cell Signaling, Danvers, MA, USA) was used, and a biotinylated secondary antibody was used (DAKO, Hamburg, Germany) for the latter. These apoptotic cardiomyocytes were detected by morphological means, as cCasp3 is a cytoplasmic staining, allowing to determine cardiomyocytes by morphology [37 (link)]. For MAC-2 and cCasp3, counterstaining was performed with Quick hemalaun kit (Vector, Burlingame, CA, USA), and for αSMAC with eosin as described [25 (link)].

For immunohistochemistry, Vectastain Elite ABC kits and diaminobenzidine (AXXORA, Lörrach, Germany) were used. Cell density was described as cells/mm², as previously reported and evaluated by a histological technician blinded for group assignment [31 (link)–33 (link)]. For mouse-derived antibodies, the mouse-on-mouse (M.O.M) immunodetection kit (AXXORA) was used. MAC-2 rat anti-mouse antibody (clone 3/38) was used for macrophages (Cedarlane, Ontario, Canada).