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Specific elisa

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Specific ELISA is a laboratory equipment used for enzyme-linked immunosorbent assay (ELISA) testing. It is designed to detect and measure specific target analytes in a sample. The core function of Specific ELISA is to quantify the presence and concentration of a particular substance or molecule of interest through a series of immunochemical reactions and colorimetric detection.

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Lab products found in correlation

Freund s adjuvant, by Merck Group (2 mentions) Anti cd40 gm csf, by BioLegend (2 mentions)

Spelling variants of this product

Mouse-specific ELISA kits (7 citations) Mouse anti-dsDNA IgG–specific ELISA kit (3 citations)

2 protocols using specific elisa

1

Generation of Anti-Siglec-15 Monoclonal Antibodies

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To generate the mAbs, NZB/NZW F1 mice were immunized with mouse Siglec-15 Fc fusion protein in Freund’s adjuvants (Sigma-Aldrich, St Louis, MO) using protocols as previously described44 (link),45 (link). Hybridoma clones (m01 and m03) were screened by mouse Siglec-15 Fc specific ELISA as well as flow cytometry using 293T-S15+ cells. A different panel of Siglec-15 hybridomas were also generated by the immunization of Siglec-15 KO mice with human Siglec-15 Fc in complete Freund’s adjuvant followed by i.p. injection of anti-CD40/GM-CSF (BioLegend), and then boosted with Sigelc15 Fc in incomplete Freund’s adjuvant. Hybridoma clones (5G12 and IH3) were selected based on their specificity to both mouse and human Siglec-15.
Mouse tissues and tumors were excised, fixed with formalin, embedded with paraffin, and stained with hematoxylin and eosin. Tissue inflammatory status was evaluated in a blind manner by pathologists. Anti-nuclear antigens Ig and anti-double stranded DNA IgG in the serum of mice were analyzed by specific ELISA (Alpha Diagnostic International, San Antonio, TX).
Wang J., Sun J., Liu L.N., Flies D.B., Nie X., Toki M., Zhang J., Song C., Zarr M., Zhou X., Han X., Archer K.A., O’Neill T., Herbst R.S., Boto A.N., Sanmamed M.F., Langermann S., Rimm D.L, & Chen L. (2019). Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy. Nature medicine, 25(4), 656-666.
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2

Generation of Anti-Siglec-15 Monoclonal Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
To generate the mAbs, NZB/NZW F1 mice were immunized with mouse Siglec-15 Fc fusion protein in Freund’s adjuvants (Sigma-Aldrich, St Louis, MO) using protocols as previously described44 (link),45 (link). Hybridoma clones (m01 and m03) were screened by mouse Siglec-15 Fc specific ELISA as well as flow cytometry using 293T-S15+ cells. A different panel of Siglec-15 hybridomas were also generated by the immunization of Siglec-15 KO mice with human Siglec-15 Fc in complete Freund’s adjuvant followed by i.p. injection of anti-CD40/GM-CSF (BioLegend), and then boosted with Sigelc15 Fc in incomplete Freund’s adjuvant. Hybridoma clones (5G12 and IH3) were selected based on their specificity to both mouse and human Siglec-15.
Mouse tissues and tumors were excised, fixed with formalin, embedded with paraffin, and stained with hematoxylin and eosin. Tissue inflammatory status was evaluated in a blind manner by pathologists. Anti-nuclear antigens Ig and anti-double stranded DNA IgG in the serum of mice were analyzed by specific ELISA (Alpha Diagnostic International, San Antonio, TX).
Wang J., Sun J., Liu L.N., Flies D.B., Nie X., Toki M., Zhang J., Song C., Zarr M., Zhou X., Han X., Archer K.A., O’Neill T., Herbst R.S., Boto A.N., Sanmamed M.F., Langermann S., Rimm D.L, & Chen L. (2019). Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy. Nature medicine, 25(4), 656-666.
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