The High-performance liquid chromatography (HPLC) system includes two LC-20AD pumps (Shimadzu, Japan), an SPD-20A UV-Vis detector, a thermostat column, and an Agilent ZORBAX SB-C18 column (5 µm, 4.6 × 250 mm). The eluation system consists of water with 0.1% formic acid (mobile phase A) and methanol (mobile phase B), and the flow rate was 0.8 mL/min during the following gradient: 0.00–35.00 min, 30–100% mobile phase B; 35.00–40.00 min 100 mobile phase B. The 1D NMR spectra were recorded in CD3OD using a Bruker DRX-600 spectrometer (Bruker, Rheinstetten, Germany) with tetramethylsilane (TMS) as the internal standard. Cells were cultured in a constant temperature incubator shaker (Zhicheng Analytical Instrument, Shanghai, China). The ligand fishing process was completed by a high-speed centrifuge (DLABsci Instrument, Beijing, China) and a vortex oscillator (Crystal Instrument, HYQ-3110, USA). HPLC-MS/MS analysis was performed on a Waters ACQUITY system coupled with a XEVO TQ MS triple-quadrupole mass spectrometer (Waters, Milford, PA, USA). A microplate reader (ThermoFisher, Multiskan GO, USA) was used for enzymatic activity assay. A Shimadzu 8030 LC-MS (Shimadzu, Japan) was used for compound identification.
Spd 20a uv vis detector
Manufactured by Agilent Technologies
Sourced in Japan
The SPD-20A UV-Vis detector is a laboratory instrument designed to detect and measure the absorbance of ultraviolet and visible light by chemical compounds. It is a core component in various analytical techniques and procedures.
Automatically generated - may contain errors
Lab products found in correlation
Lc 20ad pump, by Shimadzu (1 mentions)
Xevo tq ms triple quadrupole mass spectrometer, by Waters Corporation (1 mentions)
Drx 600 spectrometer, by Bruker (1 mentions)
Zorbax sb c18 column, by Agilent Technologies (1 mentions)
Multiskan go, by Thermo Fisher Scientific (1 mentions)
Acquity system, by Waters Corporation (1 mentions)
Inertsustain c18, by Agilent Technologies (1 mentions)
2 protocols using spd 20a uv vis detector
1
Characterization of Bioactive Compounds via HPLC-MS/MS
2
Preparative and Analytical HPLC Purification of Peptides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peptides were purified using preparative HPLC (Shimadzu, SPD-20A UV/VIS detector, LC-20AR LC pump) with a C18 column (Agilent Eclipse XDB-C18, 9.4 × 250 mm, 5 μm) with 10% MeCN (aq.)/acetonitrile (0.1% TFA) eluent system. Gradient: 0–3 min, 0% B; 3–13 min, 0–100% B to obtain a crude product of peptides and gradient: 0–3 min, 0–40% B; 3–30 min, 40–100% B for further purification.
Analytical HPLC was done using three methods. Method 1: column: Agilent Eclipse XDB-C18, 4.6 × 250 mm, 5 μm; wavelength: 254 nm; flow: 1 mL min−1; column temperature: 25 °C; eluent system: A: 10% MeCN (aq.)/B: acetonitrile (0.1% TFA) gradient: 0–3 min, 0–30% B; 3–60 min, 30–100% B. Method 2: column: Agilent Eclipse XDB-C18, 4.6 × 250 mm, 5 μm; wavelength: 254 nm; flow: 1 mL min−1; column temperature: 25 °C; eluent system: A: 10% MeOH (aq.)/B: methanol (0.1% TFA); gradient: 0–3 min, 0% B; 3–60 min, 0–100% B. Method 3: column: GL sciences, InertSustain C18, 4.6 × 250 mm, 5 μm; wavelength: 220 nm; flow: 1 mL min−1; column temperature: 45 °C; eluent system: A: 10% MeCN (aq., 0.1% TFA)/B: acetonitrile (0.1% TFA) gradient: 0–5 min, 0–30% B; 5–30 min, 30–80% B.
Analytical HPLC was done using three methods. Method 1: column: Agilent Eclipse XDB-C18, 4.6 × 250 mm, 5 μm; wavelength: 254 nm; flow: 1 mL min−1; column temperature: 25 °C; eluent system: A: 10% MeCN (aq.)/B: acetonitrile (0.1% TFA) gradient: 0–3 min, 0–30% B; 3–60 min, 30–100% B. Method 2: column: Agilent Eclipse XDB-C18, 4.6 × 250 mm, 5 μm; wavelength: 254 nm; flow: 1 mL min−1; column temperature: 25 °C; eluent system: A: 10% MeOH (aq.)/B: methanol (0.1% TFA); gradient: 0–3 min, 0% B; 3–60 min, 0–100% B. Method 3: column: GL sciences, InertSustain C18, 4.6 × 250 mm, 5 μm; wavelength: 220 nm; flow: 1 mL min−1; column temperature: 45 °C; eluent system: A: 10% MeCN (aq., 0.1% TFA)/B: acetonitrile (0.1% TFA) gradient: 0–5 min, 0–30% B; 5–30 min, 30–80% B.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!