The following mAbs and reagents were used in this study: Allophycocyanin (APC)-conjugated anti-CD3, APC-conjugated anti-IL-4, APC-conjugated anti-TCRVα24-Jα18, APC-Cy7-conjugated anti-CD3, FITC-conjugated anti-CD3, FITC-conjugated anti-CD45, FITC-conjugated anti-CD56, FITC-conjugated anti-TCRγδ, FITC-conjugated anti-IFN-γ, PE-conjugated anti-TCRVα24-Jα18, PE-conjugated anti-CD45, PE-conjugated anti-CD56, PE-conjugated anti-CD69, PE-conjugated anti-IL-17A, PE-conjugated anti-lymphocyte-activation gene 3 (anti-LAG3), PE-Cy5-conjugated anti-CD161, PE-Cy7-conjugated anti-TNF-α, PerCP-conjugated anti-CD3, PerCP-conjugated anti-CD45, FITC-conjugated mouse IgG isotype, PE-conjugated mouse IgG isotype and PE-Cy7-conjugated mouse IgG isotype control (all from Becton Dickinson, San Diego, CA, USA); PE-conjugated anti-programmed death-1 (anti-PD-1; eBioscience, San Diego, CA, USA) and APC-conjugated anti-TCR Vα7.2 (BioLegend, San Diego, CA, USA). Cells were stained with combinations of appropriate mAbs for 20 min at 4°C. Stained cells were analyzed on a Navios flow cytometer using Kaluza software (version 1.5a; Beckman Coulter, Brea, CA, USA).
Pe conjugated mouse igg isotype
Manufactured by BD
Sourced in United States
The PE-conjugated mouse IgG isotype is a laboratory reagent used for flow cytometry. It serves as an isotype control, which is a critical component in the analysis of cell surface markers. This product provides a non-specific binding control to help distinguish specific target binding from background signal.
Automatically generated - may contain errors
Lab products found in correlation
Pe cy5 conjugated anti cd161, by BD (3 mentions)
Pe cy7 conjugated anti tnf α, by BD (3 mentions)
Fitc conjugated mouse igg isotype, by BD (3 mentions)
Apc conjugated anti tcr vα7, by BioLegend (3 mentions)
Navios flow cytometer, by Beckman Coulter (3 mentions)
Apc cy7 conjugated anti cd3, by BD (2 mentions)
Pe conjugated anti cd69, by BD (2 mentions)
Pe cy7 conjugated mouse igg isotype control, by BD (2 mentions)
Pe conjugated anti programmed death 1 anti pd 1, by Thermo Fisher Scientific (2 mentions)
Kaluza software, by Beckman Coulter (2 mentions)
Pe conjugated anti il 17, by BD (2 mentions)
Fitc conjugated anti tcr γδ, by BD (2 mentions)
Fitc conjugated anti ifn γ, by BD (2 mentions)
Percp conjugated anti cd45, by BD (1 mentions)
Pe conjugated anti cd56, by BD (1 mentions)
Percp conjugated anti cd3, by BD (1 mentions)
Pe conjugated anti ccr6, by BD (1 mentions)
Fitc conjugated anti cd107a, by BD (1 mentions)
Fitc conjugated anti cd3, by BD (1 mentions)
Fitc conjugated annexin 5, by BD (1 mentions)
3 protocols using pe conjugated mouse igg isotype
1
Multiparameter Flow Cytometry Analysis
2
Multiparametric Flow Cytometry of Immune Cells
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The following mAbs and reagents were used in this study: Allophycocyanin (APC)-Alexa Fluor 750-conjugated anti-CD3, phycoerythrin (PE)-Cy5-conjugated anti-CD161, fluorescein isothiocyanate (FITC)-conjugated anti-TCR γδ, FITC-conjugated anti-IFN-γ, PE-conjugated anti-IL-17, PE-Cy7-conjugated anti-TNF-α, PE-conjugated anti-CCR6, PE-conjugated anti-CCR9, PE-conjugated anti-CXCR3, PE-conjugated anti-CXCR6, FITC-conjugated anti-perforin, FITC-conjugated anti-granzyme, FITC-conjugated anti-CD107a, FITC-conjugated mouse IgG isotype, PE-conjugated mouse IgG isotype and PE-Cy7-conjugated mouse IgG isotype control (all from Becton Dickinson, San Diego, CA) and APC-conjugated anti-TCR Vα7.2 (BioLegend, San Diego, CA). Cells were stained with combinations of appropriate mAb for 20 minutes at 4°C. Stained cells were analyzed on a Navios flow cytometer using Kaluza software (Beckman Coulter, Brea, CA).
3
Immunophenotyping of T Cell Subsets
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following mAbs and reagents were used in this study: Allophycocyanin (APC)-Cy7-conjugated anti-CD3, phycoerythrin (PE)-Cy5-conjugated anti-CD161 and fluorescein isothiocyanate (FITC)-conjugated anti-TCR γδ, FITC-conjugated anti-CD3, FITC-conjugated anti-IFN-γ, FITC-conjugated annexin V, PE-conjugated anti-CD3, PE-conjugated anti-IL-17, PE-Cy7-conjugated anti-TNF-α, PE-conjugated anti-CD69, FITC-conjugated mouse IgG isotype, PE-conjugated mouse IgG isotype and PE-Cy7-conjugated mouse IgG isotype control (all from Becton Dickinson, San Diego, CA); PE-conjugated anti-programmed death-1 (anti-PD-1; eBioscience, San Diego, CA) and APC-conjugated anti-TCR Vα7.2 (BioLegend, San Diego, CA). Cells were stained with combinations of appropriate mAbs for 20 minutes at 4°C. Stained cells were analyzed on a Navios flow cytometer using Kaluza software (version 1.1; Beckman Coulter, Brea, CA).
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