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Pe 102 1003

Manufactured by Qiagen
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The PE‐102-1003 is a laboratory equipment product manufactured by Qiagen. It is designed to perform a core function within the laboratory setting. No further details about its intended use or capabilities can be provided in an unbiased and factual manner.

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Lab products found in correlation

Iproof, by Bio-Rad (2 mentions) End repair kit, by Illumina (2 mentions) Paired end adapters, by Qiagen (2 mentions) Ultrapure ligase l603 hc l, by Qiagen (2 mentions) Ampure xp bead, by Beckman Coulter (2 mentions) Dneasy blood and tissue kit, by Covaris (2 mentions) Hiseq 2000, by Illumina (2 mentions)

2 protocols using pe 102 1003

1

Illumina Sequencing Library Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit and sheared using a Covaris E220 (Woburn, MA) (duty cycle 10, intensity 5, cycles/burst 200 and time 180 s) to ~400 base pair (bp). Sheared genomic DNA was gel purified and used as input for Illumina sequencing library prep, with end-repair using the NEBNext end-repair kit (E6050L), and A-tailing with Taq polymerase. Ligation to Illumina paired-end adapters (PE‐102-1003) was done using Ultrapure ligase (L603-HC-L) from Enzymatics, and amplification with iProof (Bio-Rad). Agencourt Ampure XP beads were used for clean-up and size selection at each step. The resulting DNA library was sequenced on a single lane of an Illumina HiSeq2000 at the Stanford Center for Genomics and Personalized Medicine.
Kelley J.L., Peyton J.T., Fiston-Lavier A.S., Teets N.M., Yee M.C., Johnston J.S., Bustamante C.D., Lee R.E, & Denlinger D.L. (2014). Compact genome of the Antarctic midge is likely an adaptation to an extreme environment. Nature Communications, 5, 4611.
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2

DNA Extraction, Shearing, and Library Prep

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit and sheared using a Covaris E220 (Woburn, MA) (duty cycle 10, intensity 5, cycles/burst 200, time 180 sec) to approximately 400 base pair (bp). Sheared genomic DNA was gel purified and used as input for Illumina sequencing library prep, with end-repair with NEBNext end-repair kit (E6050L), and A-tailing with Taq polymerase. Ligation to Illumina paired-end adapters (PE-102-1003) was done using Ultrapure ligase (L603-HC-L) from Enzymatics, and amplification with iProof (BioRad). Agencourt Ampure XP beads were used for clean-up and size selection at each step. The resulting DNA library was sequenced on a single lane of an Illumina HiSeq2000 at the Stanford Center for Genomics and Personalized Medicine.
Kelley J.L., Peyton J.T., Fiston-Lavier A.S., Teets N.M., Yee M.C., Johnston J.S., Bustamante C.D., Lee R.E, & Denlinger D.L. (2014). Compact genome of the Antarctic midge is likely an adaptation to an extreme environment. Nature Communications, 5, 4611.
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