Ab64644

Manufactured by Abcam

Sourced in United States

Ab64644 is a primary antibody that targets the protein STAT1. This antibody is designed for use in various immunoassay applications, such as Western blotting, immunohistochemistry, and immunocytochemistry. The core function of this product is to specifically bind and detect the STAT1 protein in biological samples.

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Lab products found in correlation

4 protocols using ab64644

Analyzing Transcriptional Regulators in Mycobacterial Infection

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Anti-LXRα (ab41902 Abcam), anti-ATF1 (sc270, Santa Cruz), anti-ATF3 (sc188, Santa Cruz), anti-ATF5 (sc377168, Santa Cruz), anti-RXRA (sc553, Santa Cruz), anti-RARA (sc551, Santa Cruz), anti-MEF2A (ab7606. Abcam), anti-MEF2C (ab64644, Abcam), anti-LC3 (NB100-2220, Novus), anti-β-actin (sc-1616, Santa Cruz), anti-H3K27ac (ab4729),anti-NCoR (ab24552), anti-KAT3B/p300 (ab14984), anti-KAT13A/SRC1/NCoA1 (ab84), anti-H3K4me1 (ab8895, abcam), anti-PPARγ (ab41928, Abcam), anti-Mtb-FITC (ab20962, Abcam), anti-rabbit Alexa647 (A-21245, Life Technologies), Prolong Gold with Dapi (Life Technologies), anti-rabbit-Alexa488 (Life Technologies), Bodipy 493 (Life Technologies). TO901317, ATRA and BAX Inhibiting Peptide V5 were bought from Sigma and resupended in DMSO. Nelfinavir (Nel) and Ritonavir (Rit) were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, National Institute of Allergy and Infectious Diseases. Auto Histone ChIP-seq Kit (Protein A) was bought from Diagenode.

Bouttier M., Laperriere D., Memari B., Mangiapane J., Fiore A., Mitchell E., Verway M., Behr M.A., Sladek R., Barreiro L.B., Mader S, & White J.H. (2016). Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection. Nucleic Acids Research, 44(22), 10571-10587.

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Protein Expression Analysis in Cardiac Tissue

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Apical myocardia harvested from four to six hearts per treatment group were homogenized in a lysis buffer containing phosphatase and proteinase cocktail inhibitor, in a ratio of 100:1:1, respectively. Lysates of normalized concentrations treated with reducing agents were denatured at 100°C for 10 min and separated on SDS-PAGE gels. The transferred protein bands were blocked and immunoblotted overnight in the following primary antibodies: β₁AR (ab3442; Abcam), β₂AR (ab182136; Abcam), MEF2 (ab64644; Abcam), GRK5 (ab64943; Abcam) GATA4 (ab84593; Abcam), NFAT (ab25916; Abcam), AC5 (PAC-501AP; FabGennix), AC6 (PAC-601AP; FabGennix), AC7 (PAC-701AP; FabGennix), ANP (sc-515701; Santa Cruz Biotechnology), BNP (sc-271185; Santa Cruz Biotechnology), GRK2 (sc-13143; Santa Cruz Biotechnology), pNF-κB (3033T; Cell Signaling Technology), NF-κB (8242T; Cell Signaling Technology), Cleaved Caspase-3 (9661T; Cell Signaling Technology), Collagen Type I (14695-1-AP, Proteintech), Collagen Type III (13548-1-AP, Proteintech), and GAPDH (10494-1-AP; Proteintech). Immunoblots were performed in triplicates and normalized with their respective loading controls.

Adzika G.K., Hou H., Adekunle A.O., Rizvi R., Adzraku S.Y., Li K., Deng Q.M., Mprah R., Ndzie Noah M.L., Adu-Amankwaah J., Machuki J.O., Shang W., Ma T., Koda S., Ma X, & Sun H. (2021). Amlexanox and Forskolin Prevents Isoproterenol-Induced Cardiomyopathy by Subduing Cardiomyocyte Hypertrophy and Maladaptive Inflammatory Responses. Frontiers in Cell and Developmental Biology, 9, 719351.

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ILK and MEF2C Signaling Pathway

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DMSO (Wako, KPM0962, Japan), ILK antibody (Abcam, ab15838, USA), ILK phosphorylation antibody (sc-130196), and polyclonal antibody p-MEF-2 (Ser59) (sc-13919-R) were purchased from Santa (USA); MEF2C antibody (ab64644) and α-tubulin antibody (ab15246) were purchased from Abcam (USA).

Dong Z., Pan W., Wu H., Liu D, & Cang M. (2015). Activated Integrin-Linked Kinase Negatively Regulates Muscle Cell Enhancement Factor 2C in C2C12 Cells. BioMed Research International, 2015, 748470.

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Antibody Staining Protocols for Neuronal Markers

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The rat anti-CTIP2 monoclonal antibody [25B6] (Abcam) was used at 1/500 for immunohistochemistry and 1/400 for flow cytometry. The rabbit anti-MEF2C polyclonal antibody ab64644 (Abcam) was diluted 1/500 for immunohistochemistry and 1/300 for flow cytometry. The rabbit anti-Etv1 antibody (GeneTex) was used at 1/500 dilution. The rabbit anti-NeuN polyclonal antibody (Merck Millipore) was diluted 1/800 for flow cytometry, and the mouse anti-NeuN monoclonal antibody (clone A60, Merck Millipore) was used at 1/800 dilution for flow cytometry. The mouse anti-NeuN (Chemicon) was used at 1/1,000 dilution for immunohistochemistry. The goat antibody against DCX (Santa Cruz) was diluted 1/500, and the rabbit antibody anti-calretinin (Swant) was used at 1/3,000 dilution. Secondary anti-mouse or anti-rabbit antibodies, generated in donkey or goat and conjugated to Alexa Fluor 488, 594, or 647, were purchased from Invitrogen. These antibodies were used at 1/500 dilution for cytometry and 1/1,000 for immunohistochemistry.

López-Sánchez N., Fontán-Lozano Á., Pallé A., González-Álvarez V., Rábano A., Trejo J.L, & Frade J.M. (2017). Neuronal tetraploidization in the cerebral cortex correlates with reduced cognition in mice and precedes and recapitulates Alzheimer's-associated neuropathology. Neurobiology of aging, 56.

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