OCs and OBs were seeded and cultured in µ-Slide 8 wells (ibidi GmbH, Lochhamer Schlag, Germany) under the same conditions described in Section 2.4 . Twenty-four hours after CNM exposure, the cultures were washed twice with Dulbecco’s phosphate-buffered saline (DPBS; Nacalai Tesque) and subsequently stained with a nuclear staining solution (bisbenzimide H33342 fluorochrome trihydrochloride dimethyl sulfoxide solution; Nacalai Tesque) and lysosome staining solution (CytoPainter lysosomal staining ab138895; Abcam, Cambridge, UK) for 30 min. The cells were then washed twice with DPBS. The images were acquired using a BZ-X710 inverted fluorescent phase-contrast microscope (Keyence, Osaka, Japan).
Bisbenzimide h33342 fluorochrome trihydrochloride dimethyl sulfoxide solution
Manufactured by Nacalai Tesque
Sourced in United Kingdom
Bisbenzimide H33342 fluorochrome trihydrochloride dimethyl sulfoxide solution is a fluorescent stain used for the detection and visualization of DNA in various applications. It is a cell-permeant nuclear counterstain that binds to the minor groove of DNA, emitting a blue fluorescence upon excitation. This product is commonly used in microscopy, flow cytometry, and other techniques for nuclear staining and DNA quantification.
Automatically generated - may contain errors
2 protocols using bisbenzimide h33342 fluorochrome trihydrochloride dimethyl sulfoxide solution
1
Fluorescent Imaging of Nanoparticle-Treated Cells
2
Assessing Cellular Lysosomal Response to Nanomaterials
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Cells cultured on Cellview glass-bottomed advanced TC four compartments (Greiner Bio-one, Frickenhausen, Germany) were exposed to FT9110 and CNHs at 100 µg/mL for 48 hours under the same conditions as described for the cell viability assay. For assessment, cells were stained with nuclear stain (Bisbenzimide H33342 Fluorochrome Trihydrochloride Dimethyl Sulfoxide Solution; Nacalai Tesque) and lysosome stain (Cyto Painter Lysosomal Staining ab 138895; Abcam, Cambridge, UK) 30 minutes before observation. The cells were visualized using an AxioObserverZ1 fluorescence microscope (Carl Zeiss Jena GmbH, Jena, Germany) with a 40× objective lens.
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