Platelets isolated by gel filtration (2×108/mL) and treated with indicated inhibitors and agonists were pelleted at 960 g (5 min, 37°C). The platelets were then lysed for 30 min on ice with 50 mM Tris-HCl (pH 7.4), 1 % IGEPAL CA-630, 0.25 % sodium deoxycholate, 150 mM NaCl, 1 mM EDTA, 1 mM PMSF, 1 µg/mL aprotinin, 1 µg/mL leupeptin, 1 µg/mL pepstatin, 1 mM Na3VO4, and 1 mM NaF. Proteins were separated by SDS-PAGE, transferred to PVDF membranes, and probed with rabbit anti-human phospho-PLCγ2 Tyr759, phospho-PLCγ2 Tyr1217, phospho-SYK Tyr323, phospho-SYK Tyr525/526 (C87C1), or phospho-Src family Tyr416 (Cell Signaling, Danvers, MA). The membranes were stripped and then probed for normalization with rabbit anti-human SYK (C-20) (Santa Cruz Biotechnology, Santa Cruz, CA) or PLCγ2 (Cell Signaling).
Phospho plcγ2 tyr759
Manufactured by Cell Signaling Technology
Sourced in United States
Phospho-PLCγ2 (Tyr759) is a rabbit monoclonal antibody that recognizes the phosphorylated form of phospholipase C gamma 2 (PLCγ2) at the tyrosine 759 residue. This antibody is designed for the detection and analysis of the phosphorylation status of PLCγ2 in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Plcγ2, by Cell Signaling Technology (2 mentions)
Phospho tyr1217 plcγ2, by Cell Signaling Technology (1 mentions)
Phospho src family tyr416, by Cell Signaling Technology (1 mentions)
Phospho syk tyr525 526 c87c1, by Cell Signaling Technology (1 mentions)
Anti actin antibody, by Merck Group (1 mentions)
C57bl 6n mice, by Orient Bio (1 mentions)
2 protocols using phospho plcγ2 tyr759
1
Platelet signaling pathway analysis
2
Osteoclastogenesis Signaling Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The C57BL/6N mice used in this study were purchased from Orient Bio Inc. (Seongnam, Korea). All mouse studies were performed following the protocol (WKU16-87) approved by the Animal Care and Use Committee of Wonkwang University.
Cell culture agents, including media, fetal bovine serum (FBS), and supplements, were purchased from Hyclone (Pierce, Rockford, IL, USA). Soluble recombinant mouse RANKL and recombinant human macrophage colony-stimulating factor (M-CSF) were supplied by T Kim (KIOM, Daejeon, Korea). Anti-NFATc1 and anti-c-Fos antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and anti-actin antibody from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against other proteins used in this study (phospho-ERK [p-ERK; Thr202/Tyr204], ERK, phospho-JNK [Thr183/Tyr185], JNK, p-p38 [Thr180/Tyr182], p-38, phospho-IκBα [Ser32], IκBα, phospho-BTK [Ser180], BTK, phospho-PLCγ2 [Tyr759], and PLCγ2) were purchased from Cell Signaling Technology (Danvers, MA, USA).
Cell culture agents, including media, fetal bovine serum (FBS), and supplements, were purchased from Hyclone (Pierce, Rockford, IL, USA). Soluble recombinant mouse RANKL and recombinant human macrophage colony-stimulating factor (M-CSF) were supplied by T Kim (KIOM, Daejeon, Korea). Anti-NFATc1 and anti-c-Fos antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and anti-actin antibody from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against other proteins used in this study (phospho-ERK [p-ERK; Thr202/Tyr204], ERK, phospho-JNK [Thr183/Tyr185], JNK, p-p38 [Thr180/Tyr182], p-38, phospho-IκBα [Ser32], IκBα, phospho-BTK [Ser180], BTK, phospho-PLCγ2 [Tyr759], and PLCγ2) were purchased from Cell Signaling Technology (Danvers, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!