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Aldh1 2

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ALDH1/2 is a laboratory reagent used in biochemical research. It is an enzyme that catalyzes the oxidation of aldehydes to carboxylic acids. The core function of ALDH1/2 is to facilitate this specific enzymatic reaction.

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Lab products found in correlation

P21 waf1 cip1 12d1, by Cell Signaling Technology (1 mentions) Cd133, by Miltenyi Biotec (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) Mouse anti human cd44, by Cell Signaling Technology (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Cyclin d1, by Thermo Fisher Scientific (1 mentions) Neutral buffered formalin, by Merck Group (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Vectastain abc kit, by Vector Laboratories (1 mentions) Nucleoporin 62, by Santa Cruz Biotechnology (1 mentions) Abcg2, by Cell Signaling Technology (1 mentions) Cd133, by Cell Signaling Technology (1 mentions) Epcam, by Cell Signaling Technology (1 mentions) Aldh1, by Cell Signaling Technology (1 mentions) Nanog, by Cell Signaling Technology (1 mentions) Mdr1 abcb1, by Cell Signaling Technology (1 mentions) Epas 1, by Santa Cruz Biotechnology (1 mentions) Hif 1α, by Cell Signaling Technology (1 mentions)

4 protocols using aldh1 2

1

Western Blot Analysis of EMT Markers

Cited 2 times
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The cell lysis, determination of protein concentrations, protein separation by SDS-PAGE, and blotting procedures were performed as previously described [39 (link),40 (link)]. For the detection of specific protein bands, the following antibodies were used: p21 Waf1/Cip1 (12D1, Cell Signaling #2947, 1:2000), ZEB1 (D80D3, Cell Signaling #3396, 1:500), Vimentin (D21H3, Cell Signaling #5741, 1:5000), E-cadherin (24E10, Cell Signaling #3195, 1:2000), SNAI2 (Slug, C19G7, Cell Signaling #9585, 1:1000), CD133 (Miltenyi #130-092-395, 1:250), ALDH1/2 (Santa Cruz #sc-166362, 1:500), CD44 (Cell Signaling #3570, 1:1000), ABCG2 (Cell Signaling # 4477, 1:1000), Oct4A (C52G3, Cell Signaling #2890, 1:1000), and GAPDH (6C5, Abnova #MAB5476, 1:40,000). Protein bands were visualized using Immobilon Western Blot Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA). Images were processed and analyzed using ImageJ (ImageJ 1.46r, Rasband, WS, U.S. National Institutes of Health) and ratios were determined relative to the intensity of the housekeeper GAPDH band. The uncropped western blot figures can been found in supplementary files.
Bueno-Fortes S., Muenzner J.K., Berral-Gonzalez A., Hampel C., Lindner P., Berninger A., Huebner K., Kunze P., Bäuerle T., Erlenbach-Wuensch K., Sánchez-Santos J.M., Hartmann A., De Las Rivas J, & Schneider-Stock R. (2021). A Gene Signature Derived from the Loss of CDKN1A (p21) Is Associated with CMS4 Colorectal Cancer. Cancers, 14(1), 136.
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2

Red Propolis Modulates Stemness Markers

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UM-SCC-17B cells were treated with increasing concentrations of red propolis (0.5-50 μg/ml) for 24 hours. Alternatively, cells were exposed to 5 μg/ml red propolis or vehicle control for 24, 48, or 72 hours. Primary antibodies were, as follows: rabbit anti-human Bmi-1 or Oct-4 (Cell Signaling Technology); mouse anti-human CD44 (Cell Signaling Technology), ALDH1/2 (Santa Cruz Biotechnology) or β-actin (Chemicon/Millipore). Immunoreactive proteins were visualized by SuperSignal West Pico chemiluminescent substrate (Thermo Scientific).
Nör F., Nör C., Bento L.W., Zhang Z., Bretz W.A, & Nör J.E. (2021). Propolis Reduces the Stemness of Head and Neck Squamous Cell Carcinoma. Archives of oral biology, 125, 105087.
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3

Immunohistochemical Analysis of Thyroid Cancer

Cited 1 time
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Formalin-fixed, paraffin-embedded (FFPE) tissues from surgically removed human thyroid cancer samples were collected at Asan Medical Center, Seoul, Korea between 1996 and 2012 following the Institutional Review Board approval. The use of human samples was also approved by the IRB board of the National Cancer Institute. Tissues from xenografted tumor were fixed in 10% neutral buffered formalin (Sigma-Aldrich) and subsequently embedded in paraffin. Five-micrometer-thick sections were prepared and stained with hematoxylin and eosin. Immunohistochemistry (IHC) was performed as previously described (Kim, et al. 2012 (link)) with some modification using Vectastain ABC kit (Vector Laboratories). Antibodies for SRC-3 (#611104, BD Transduction Laboratories), Cyclin D1 (RB-9041-P1, Thermo Scientific), Ki-67 (RB-9043-P0, Thermo Scientific), Bim (#2933, Cell Signaling), cleaved caspase-3 (#9664, Cell Signaling), ALDH1/2 (sc-166362, Santa Cruz), and β-catenin (#9562, Cell Signaling) were used to evaluate protein expression by IHC.
Lee W.K., Kim W.G., Fozzatti L., Park S., Zhao L., Willingham M.C., Lonard D., O’Malley B.W, & Cheng S.Y. (2020). Steroid receptor coactivator-3 as a target for anaplastic thyroid cancer. Endocrine-related cancer, 27(4), 209-220.
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4

Western Blot Analysis of Stem Cell Markers

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Total cell lysates were obtained by adding RIPA buffer (R0728, Sigma-Aldrich) and protease inhibitor cocktail (P2714, Sigma-Aldrich). Equilibrated protein samples were loaded into a 10% SDS-Page gel, electrophoresed and then electro-transferred to a PVDF membrane (Bio-Rad). Transferred membranes were blocked with a blocking buffer containing 5% non-fat milk (Labscientific, M0841). The following monoclonal antibodies were used: Nanog (Cell Signaling, D73G4), CD133 (Cell Signaling, D2V8Q), CD44 (Cell Signaling, 8E2), ALDH1/2 (Santa Cruz, sc-166362), ALDH1 (Cell Signaling, D9J7R), MDR1/ABCB1 (Cell Signaling, E1Y7B), HIF1-α (Cell Signaling, D5F3M), ABCG2 (Cell Signaling, D5V2K), nucleoporin-62 (Santa Cruz, sc-48373), EpCAM (Cell Signaling, VU1D9) and EPAS-1 (Santa Cruz, 190B). Target proteins were visualized with the secondary mouse or rabbit IgG antibodies and a chemiluminescent substrate (Santa Cruz, sc-2048). All Western blot results were normalized by total protein (Fig. S5). All Western blot experiments were reproduced and performed three times for quantification.
Shen Q., Hill T., Cai X., Bui L., Barakat R., Hills E., Almugaiteeb T., Babu A., Mckernan P.H., Zalles M., Battiste J.D, & Kim Y.T. (2021). Physical Confinement during Cancer Cell Migration Triggers Therapeutic Resistance and Cancer Stem Cell-like Behavior. Cancer letters, 506, 142-151.
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