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Kb nb 142 70

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The Kb-NB-142-70 is a laboratory equipment product manufactured by Bio-Techne. It is a precision instrument designed for specialized applications in research and development laboratories. The core function of this product is to provide accurate and consistent measurements or analysis, but a detailed description of its intended use cannot be provided without the risk of extrapolation or bias.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Rat tail collagen 1, by BD (2 mentions) Dexamethasone (dex), by Merck Group (2 mentions) Collagenase 1, by Thermo Fisher Scientific (2 mentions) Soybean trypsin inhibitor, by Thermo Fisher Scientific (2 mentions) Phenol red free matrigel, by BD (2 mentions) Anti ras antibody, by Abcam (2 mentions) Anti amylase and anti β actin antibodies, by Merck Group (2 mentions) Anti cytokeratin 19 antibody, by Leica (2 mentions) Anti claudin 18 antibody, by Thermo Fisher Scientific (2 mentions) Anti gst and anti gfp, by Santa Cruz Biotechnology (2 mentions) Anti activated notch1 antibody, by Abcam (2 mentions) Anti pkd2, by OriGene (2 mentions) Anti pkd3 antibody, by Fortis Life Sciences (2 mentions) Anti ps744 748 pkd antibodies, by Cell Signaling Technology (2 mentions) Recombinant human tgfα, by Merck Group (2 mentions) Crt0066101, by Bio-Techne (2 mentions) Anti pkd1, by Antibodies-online (2 mentions) Transit 293, by Mirus Bio (1 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions)

5 protocols using kb nb 142 70

1

HEK293T Transient Transfection Assay

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HEK293T cells were maintained in RPMI 1640 medium supplemented with 10% FCS. For transient transfections, 3·105 HEK293T cells were seeded per well of a 6-well plate. The next day, cells were transfected with 2 μg of plasmid DNA using TransIT293 (Mirus Bio Corporation, Madison, WI) according to the manufacturer’s instructions and grown for 24 hours. Treatment of cells with kbNB142-70 (Tocris) and PDBu (Sigma-Aldrich) was at 10 μM and 1 μM, respectively.
Weber P., Hornjik M., Olayioye M.A., Hausser A, & Radde N.E. (2015). A computational model of PKD and CERT interactions at the trans-Golgi network of mammalian cells. BMC Systems Biology, 9, 9.
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2

Antibody Source and Cell Culture Reagents

Cited 1 time
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The anti-amylase and anti-β-actin antibodies were from Sigma-Aldrich (St Louis, MO), anti-cytokeratin-19 antibody from Leica Microsystems (Buffalo Grove, IL), anti-claudin-18 antibody from Invitrogen (Carlsbad, CA), anti-Ras antibody from Epitomics (Burlingame, CA), anti-GST and anti-GFP from Santa Cruz (Santa Cruz, CA), anti-activated Notch1 antibody from Abcam (Cambridge, MA), anti-PKD1 from Antibodies-online Inc. (Atlanta, GA), anti-PKD2 from Acris Antibodies (San Diego, CA), anti-PKD3 antibody from Bethyl Laboratories (Montgometry, TX), and anti-pS744/748-PKD antibodies from Cell Signaling Technology (Danvers, MA) or Abcam. Recombinant human TGFα was purchased from Chemicon (Billerica, MA) and R&D Systems (Minneapolis, MN). Dexamethasone was from Sigma-Aldrich, soybean trypsin inhibitor and collagenase I from Affymetrix (Santa Clara, CA). Phenol red-free Matrigel and rat tail collagen I were from BD Biosciences (San Diego, CA). Hoechst 33342 was purchased from Invitrogen (Grand Island, NY). The PKD inhibitor CRT0066101 was from TOCRIS (Ellisville, MO) and kb-NB-142-70 was described previously 51 (link). The γ-secretase inhibitors DAPT and R04929097 were from Cellagen Technology (San Diego, CA).
Liou G.Y., Döppler H., Braun U.B., Panayiotou R., Buzhardt M.S., Radisky D.C., Crawford H.C., Fields A.P., Murray N.R., Wang Q.J., Leitges M, & Storz P. (2015). Protein kinase D1 drives pancreatic acinar cell reprogramming and progression to intraepithelial neoplasia. Nature communications, 6, 6200.
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3

Antibody Source and Cell Culture Reagents

Cited 1 time
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The anti-amylase and anti-β-actin antibodies were from Sigma-Aldrich (St Louis, MO), anti-cytokeratin-19 antibody from Leica Microsystems (Buffalo Grove, IL), anti-claudin-18 antibody from Invitrogen (Carlsbad, CA), anti-Ras antibody from Epitomics (Burlingame, CA), anti-GST and anti-GFP from Santa Cruz (Santa Cruz, CA), anti-activated Notch1 antibody from Abcam (Cambridge, MA), anti-PKD1 from Antibodies-online Inc. (Atlanta, GA), anti-PKD2 from Acris Antibodies (San Diego, CA), anti-PKD3 antibody from Bethyl Laboratories (Montgometry, TX), and anti-pS744/748-PKD antibodies from Cell Signaling Technology (Danvers, MA) or Abcam. Recombinant human TGFα was purchased from Chemicon (Billerica, MA) and R&D Systems (Minneapolis, MN). Dexamethasone was from Sigma-Aldrich, soybean trypsin inhibitor and collagenase I from Affymetrix (Santa Clara, CA). Phenol red-free Matrigel and rat tail collagen I were from BD Biosciences (San Diego, CA). Hoechst 33342 was purchased from Invitrogen (Grand Island, NY). The PKD inhibitor CRT0066101 was from TOCRIS (Ellisville, MO) and kb-NB-142-70 was described previously 51 (link). The γ-secretase inhibitors DAPT and R04929097 were from Cellagen Technology (San Diego, CA).
Liou G.Y., Döppler H., Braun U.B., Panayiotou R., Buzhardt M.S., Radisky D.C., Crawford H.C., Fields A.P., Murray N.R., Wang Q.J., Leitges M, & Storz P. (2015). Protein kinase D1 drives pancreatic acinar cell reprogramming and progression to intraepithelial neoplasia. Nature communications, 6, 6200.
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4

Angiogenesis Inhibition Mechanism Study

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Axitinib was obtained from M/S. Eurasian Chemicals PVT. Ltd. and kb-NB142-70 from Tocris Bioscience (UK). VEGF165 was purchased from Calbiochem (Merck-Millipore, Germany). Antibodies against phospho-Ser744/748 PKD1, phospho-Ser916 PKD1, total PKD1, phospho-Tyr951 VEGFR2, total VEGFR2, and β-actin were from Cell Signaling Technology. Phospho-Ser498 HDAC5 and total HDAC5 antibodies were from Santa Cruz Biotechnology. Phorbol 12-myristate 13-acetate (PMA) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich.
Varga A., Gyulavári P., Greff Z., Futosi K., Németh T., Simon-Szabó L., Kerekes K., Szántai-Kis C., Brauswetter D., Kokas M., Borbély G., Erdei A., Mócsai A., Kéri G, & Vántus T. (2015). Targeting Vascular Endothelial Growth Factor Receptor 2 and Protein Kinase D1 Related Pathways by a Multiple Kinase Inhibitor in Angiogenesis and Inflammation Related Processes In Vitro. PLoS ONE, 10(4), e0124234.
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5

Calcium Signaling Modulation via Pharmacological Compounds

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The NT solution contained (in mM) NaCl (143), KCl (5.4), CaCl2 (1.8), MgCl2 (0.5), NaH2PO4 (0.5), glucose (11.1), and HEPES (5) and was adjusted to pH 7.4 with NaOH. Fura 2‐AM was obtained from Thermo Fisher Scientific; RvD1, RvE1, and compound A (Cpd A) were obtained from Cayman Chemical; bisindolylmaleimide I (BIM I) and BIM V were obtained from Merck; DC260126, AH‐7614, GW‐9508, and kb‐NB 142‐70 were obtained from Tocris Bioscience; and TAK‐875 and AM‐1638 were obtained from MedChemExpress. All other drugs were purchased from Sigma‐Aldrich. Stock solutions of the drugs were made by dissolving the compounds in deionized water or dimethyl sulfoxide (DMSO) according to the manufacturers' specifications and stored at −20°C. On the day of the experiment, one aliquot was thawed and used. The final concentration of DMSO in the solutions was <0.1%.
Pyo H., An X, & Cho H. (2022). The role of free fatty acid receptor pathways in a selective regulation of TRPA1 and TRPV1 by resolvins in primary sensory neurons. Journal of Cellular Physiology, 237(9), 3651-3660.
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