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Fei quanta 250

Manufactured by Thermo Fisher Scientific
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Sourced in United States, Netherlands, France

The FEI Quanta 250 is a scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of samples. It features a field emission electron source, which provides high-brightness and high-resolution imaging capabilities. The Quanta 250 is capable of operating in high-vacuum, low-vacuum, and environmental modes, allowing for the examination of both conductive and non-conductive samples.

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Lab products found in correlation

Exoquick exosome precipitation solution, by System Biosciences (3 mentions) Leica em cpd030, by Leica Microsystems (2 mentions) Leica em scd050, by Leica Microsystems (2 mentions) Leica em ace200, by Leica Microsystems (2 mentions) Zetaview pmx 110, by Particle Metrix (2 mentions) Oxoid cm0003, by Merck Group (2 mentions) Zetasizer nano, by Malvern Panalytical (1 mentions) Ftir 8400, by Shimadzu (1 mentions) Uv 1800, by Shimadzu (1 mentions) Odn1668, by InvivoGen (1 mentions) Dxr 500, by Bruker (1 mentions) Scanning electron microscope, by Thermo Fisher Scientific (1 mentions) Jxa 8100, by JEOL (1 mentions) Sdc 050 sputter coater, by Thermo Fisher Scientific (1 mentions) 0.1 m sodium cacodylate buffer, by Merck Group (1 mentions) Glutaraldehyde, by Merck Group (1 mentions) Evo 10, by Zeiss (1 mentions) Cpd 030, by Leica Microsystems (1 mentions) Scd050, by Leica Microsystems (1 mentions) Leo 906e, by Zeiss (1 mentions)

60 protocols using fei quanta 250

1

Liposomal Formulation and Cell Morphology Analysis

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Morphology of the liposomes of DODAC/PHO-S (1:1) (0.3–2.0 mM) and empty DODAC (0.3–2.0 mM) was analyzed by scanning electron microscopy (SEM). Aliquots of 5 μL of the liposomal formulation in a glass slides were incubated for 1 hour at 50°C. The samples were gold coated using a Balzers SCD 030 Sputter Coater (Balzers Union Ltd., Balzers, Liechtenstein) and visualized in an FEI Quanta 250 (FEI Company, Hillsboro, OR, USA) SEM.
Aliquots of melanoma B16F10 cells (5×104 cells/well, when the cell density reached 80%–90% confluence) were plated on sterile glass slides. After adhesion, cells were treated with PHO-S, empty DODAC, and DODAC/PHO-S (1:1) (0.3 and 2.0 mM). Then, the cells were fixed by adding 3% glutaraldehyde, incubated for 1 hour at 4°C, and post-fixed in 1% OsO4 for 1 hour at room temperature. After fixation, the cells were washed several times with 0.005 M sodium cacodylate, pH 7.2, and gradually passed through acetone solutions of increasing concentrations ranging from 30% to 100% for 10 minutes. Samples were completely dried in a critical-point drying apparatus using liquid CO2 as the exchange medium. Dehydrated specimens were mounted onto aluminum stubs, coated with carbon/gold and gold-coated using a Balzers SCD 030 Sputter Coater (Balzers Union Ltd.), and visualized in an FEI Quanta 250 (FEI Company) SEM.
Luna A.C., Saraiva G.K., Filho O.M., Chierice G.O., Neto S.C., Cuccovia I.M, & Maria D.A. (2016). Potential antitumor activity of novel DODAC/PHO-S liposomes. International Journal of Nanomedicine, 11, 1577-1591.
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2

Biofilm Formation on Cotton Fibers

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Scanning electron microscopy (SEM) was undertaken to observe the formation of biofilms on the surface of cotton fibers. Cotton‐containing cultures of P. luteoviolacea 2ta16, P. piscicida JCM 20779, and P. rubra DSM‐6842 were prepared as described previously. Parallel cultures were allowed to grow for either 24 or 96 h at 30°C with shaking at 180 RPM. Cotton balls were then taken from the cultures and were washed with 50 mL of milli‐Q H2O by shaking at 100 RPM in an Erlenmeyer flask for 15 min at 22°C. Cells were fixed by serial washes (50 mL each) with 10% (v/v) ethanol, 25% (v/v) ethanol, 50% (v/v) ethanol, 75% (v/v) ethanol, 90% (v/v) ethanol, and 100% (v/v) ethanol for 15 min each with shaking at 80 RPM at 22°C. Samples were then directly analyzed using an FEI Quanta 250 instrument (operated at 0.6 torr, electron beam operated at 10 kV, Backscatter Electron and Secondary Electron Detectors utilized). SEM images of unused cotton balls were obtained as a control. Unused cotton balls were sputter‐coated with gold and analyzed using the FEI Quanta 250 under identical conditions.
Timmermans M.L., Picott K.J., Ucciferri L, & Ross A.C. (2018). Culturing marine bacteria from the genus Pseudoalteromonas on a cotton scaffold alters secondary metabolite production. MicrobiologyOpen, 8(5), e00724.
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3

Elemental Content Analysis of Active Carbons

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Scanning electron microscopy (SEM) was used to the elemental content of the active carbons determination. The activated carbons samples (AC1, AC2, and AC3) were observed using a Quanta 250 FEI scanning electron microscope (Thermo Fisher Scientific Inc., USA) operated at 15 kV. The elemental analysis was performed by means of a Tracor Northern energy dispersive X-ray (EDX) spectrometer (Thermo Fisher Scientific Inc., USA) mounted on the Quanta 250 FEI. The EDX detector was equipped with an ultra-thin light-element window to detect elements with atomic numbers >4. The elemental content results are presented as the mean of content from six independent places on active carbon surface. The results are presented as the percentage of element weight on the active carbon surface ± SD.
Burchacka E., Pstrowska K., Bryk M., Maciejowski F., Kułażyński M, & Chojnacka K. (2023). The Properties of Activated Carbons Functionalized with an Antibacterial Agent and a New SufA Protease Inhibitor. Materials, 16(3), 1263.
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4

Quantifying Osteoclast-Mediated Bone Resorption

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BMMs (2 × 104 cells per well) were seeded on bovine bone slices in 96-well plates for 24 h, and then stimulated with 0, 0.2, 0.4, and 0.8 µM RA in the presence of M-CSF (30 ng/mL) and RANKL (50 ng/mL) for another 3 days. Cells were then fixed with 2.5% glutaraldehyde. Bone slices were visualized under a scanning electron microscope (SEM, FEI Quanta 250; FEI, Hillsboro, OR, USA), and the resorption areas were quantified with Image J software (NIH).
Wang Q., Mo J., Zhao C., Huang K., Feng M., He W., Wang J., Chen S., Xie Z., Ma J, & Fan S. (2018). Raddeanin A suppresses breast cancer-associated osteolysis through inhibiting osteoclasts and breast cancer cells. Cell Death & Disease, 9(3), 376.
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5

Hydroxyapatite Crystal Morphology Analysis

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To examine and correctly establish the morphology and size of the hydroxyapatite crystals obtained, the hydroxyapatite samples were studied in High Vacuum mode using a FEI Quanta 250 scanning electron microscope (FEI Company, Eindhoven, The Netherlands). The analyzed samples were metallized by coating with a 5 nm thick Au layer.
Cursaru L.M., Iota M., Piticescu R.M., Tarnita D., Savu S.V., Savu I.D., Dumitrescu G., Popescu D., Hertzog R.G, & Calin M. (2022). Hydroxyapatite from Natural Sources for Medical Applications. Materials, 15(15), 5091.
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6

Scanning Electron Microscopy Imaging

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The sample surfaces were imaged by SEM using a FEI Quanta 250 (FEI Company Inc. Thermo-Fisher Scientific, OR, USA), with a tungsten filament and an acceleration voltage in the range of 10–30 kV in secondary electron mode.
Diaz-Rodriguez S., Chevallier P., Paternoster C., Montaño-Machado V., Noël C., Houssiau L, & Mantovani D. (2019). Surface modification and direct plasma amination of L605 CoCr alloys: on the optimization of the oxide layer for application in cardiovascular implants. RSC Advances, 9(4), 2292-2301.
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7

Morphological Analysis of BCP-1 Treated Cells

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For morphological analysis, MCF-7 and MDA-MB-231 cells were plated on glass coverslips in 24-well plates. Cells were then treated with BCP-1 for 48 h, then fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.3) for 90 min. Samples were then dehydrated in an increasing concentration series of ethanol (30–100%), subjected to the critical point, metallized, and analyzed under SEM (FEI Quanta 250; FEI Company, OR, USA).
Zani C.P., Zani A.P., Thomazini C.M., Retamiro K.M., de Oliveira A.R., Gonçalves D.L., Sarragiotto M.H., Garcia F.P., de Oliveira Silva S., Nakamura C.V, & Ueda-Nakamura T. (2023). β-Carboline-α-aminophosphonate Derivative: A Promising Antitumor Agent for Breast Cancer Treatment. Molecules, 28(9), 3949.
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8

Scanning Electron Microscopy of Listeria monocytogenes Biofilms

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Small (5 mm diameter x 1 mm thickness) pieces of cantaloupe rind containing L. monocytogenes biofilms were used for SEM (Scanning Electron Microscope FEI Quanta 250). Briefly, rind pieces were fixed for 2 h in 2% glutaraldehyde-PBS buffer at room temperature, followed by dehydration steps in a series of ethanol baths (10 min each) containing 25, 50, 75, 95, and 100% (3 times) ethanol, and postfixed by incubation with 1% osmium tetroxide in PBS for 1 h in the dark. The samples were then dried using Balzers Critical Point Dryer (CPD 020) before coating with gold-palladium, as described earlier (Muravnik et al., 2016 (link)).
Fulano A.M., Elbakush A.M., Chen L.H, & Gomelsky M. (2023). The Listeria monocytogenes exopolysaccharide significantly enhances colonization and survival on fresh produce. Frontiers in Microbiology, 14, 1126940.
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9

Surface Morphology of Pretreated Biomass

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Surface images of pretreated SOB and PP, with and without enzymatic digestion, were obtained by scanning electron microscopy (SEM) and compared with the untreated material. The samples were directly placed on graphite layer and observed at magnifications of x800–2000 in a scanning electron microscope model FEI Quanta-250 (FEI Co., Netherlands). Several images were obtained from different areas of the samples (at least 20 images per sample) to confirm the reproducibility of the results. Microscopy observations were made at the Microscopy Laboratory of CICVyA, INTA, Bs As, Argentina.
Ben Guerrero E., Arneodo J., Bombarda Campanha R., Abrão de Oliveira P., Veneziano Labate M.T., Regiani Cataldi T., Campos E., Cataldi A., Labate C.A., Martins Rodrigues C, & Talia P. (2015). Prospection and Evaluation of (Hemi) Cellulolytic Enzymes Using Untreated and Pretreated Biomasses in Two Argentinean Native Termites. PLoS ONE, 10(8), e0136573.
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10

Characterization of Mesoporous Silica Nanoparticles

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For this purpose, the samples were prepared by pressing it into KBr pellets in a ratio of 2:8. It was then scanned between 4000 and 400 cm−1 with a resolution of 4 cm−1. Morphological characteristics of the MSNPs and DDMSNPs were observed by scanning electron microscope (SEM) model FEI Quanta 250 (USA). Before recording the images, the samples were sputter coated with gold and then observed by SEM. The concentration of DIM and DOX was determined using UV–Vis spectrophotometer (Shimadzu UV 1800, Japan). To determine the morphology of MSNPs and DDMSNPs, JEOL JEM-2100 transmission electron microscope (TEM) (JEOL, Inc., Peabody, MA, USA) was used at an acceleration voltage of 300 kV. A drop of nanoparticle suspension was dispersed in DI water, lyophilized and mounted on a thin film of amorphous carbon deposited on a copper grid (300 meshes). After that, the grids were dried in clean condition and examined directly with the TEM. Nano Zetasizer (Malvern Instruments, Malvern, UK) was applied to perform dynamic light scattering (DLS) to identify the size of MSNPs. The most important structural groups responsible for functionality were identified by analyzing the infrared spectrum obtained in the transmission mode with a Fourier transform infrared spectrometer (FTIR) (FTIR-8400S, Shimadzu).
Sarkar R., Biswas S., Ghosh R., Samanta P., Pakhira S., Mondal M., Dutta Gupta Y., Bhandary S., Saha P., Bhowmik A, & Hajra S. (2024). Exosome-sheathed porous silica nanoparticle-mediated co-delivery of 3,3′-diindolylmethane and doxorubicin attenuates cancer stem cell-driven EMT in triple negative breast cancer. Journal of Nanobiotechnology, 22, 285.
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