Nanopure diamond water purification system

All the chemicals used for this study were of analytical grade. Internal standards and reagents for GC-MS, such as l-alanine-2,3,3,3-d₄ (d₄-alanine), d-ribitol, 12-bromo-1-dodecanol, N-methyl-N (trimethylsilyl)trifluoroamide (MSTFA), pyridine, diethyl ether, and all other metabolite standards, were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methoxyamine hydrochloride was obtained from Fluka (Steinheim, Switzerland), anhydrous sodium sulfate from BDH chemicals (Poole, UK), and acetone from Biolab (Scoresby, Australia). Methanol, sodium hydroxide and sodium bicarbonate were purchased from Merck (Darmstadt, Germany). All the chemical solutions were prepared using Grade 1 water (BARNSTEAD^® NANOpure Diamond™ Water Purification System, Waltham, MA, USA) or absolute ethanol (UNIVAR, AJAX FINECHEM, Auckland, New Zealand).

All the chemicals used for this study were of analytical grade. Internal standard and reagents for GC-MS, D-ribitol, N-methyl-N (trimethylsilyl) trifluoroamide (MSTFA), pyridine, and all other metabolite standards, were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methoxyamine hydrochloride was obtained from Fluka (Steinheim, Switzerland), anhydrous sodium sulphate from BDH chemicals (Poole, UK), and acetone from Biolab (Scoresby, Australia). Methanol, sodium hydroxide and sodium bicarbonate were purchased from Merck (Darmstadt, Germany). All the chemical solutions were prepared using Grade 1 water (Barnstead ^® NANOpure Diamond™ Water Purification System, Waltham, MA, USA) or absolute ethanol (Univar, Ajax Finechem, Auckland, New Zealand).