Anti tsp 1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-TSP-1 is a laboratory reagent used for the detection and quantification of Thrombospondin-1 (TSP-1) in various sample types. TSP-1 is a multifunctional glycoprotein involved in various cellular processes. Anti-TSP-1 can be used in techniques such as ELISA, Western Blotting, and Immunohistochemistry to measure TSP-1 levels.

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Lab products found in correlation

Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Anti vcam 1, by BioLegend (1 mentions) Collagenase type 1, by Worthington (1 mentions) Collagenase type 1a, by Merck Group (1 mentions) Tnf α, by R&D Systems (1 mentions) Streptomycin, by Lonza (1 mentions) L glutamine, by Lonza (1 mentions) Anti ve cadherin, by BD (1 mentions) Endothelial growth supplements, by Cell Biologics (1 mentions) Chemi doc xrs imaging device, by Bio-Rad (1 mentions) Anti myc, by Cell Signaling Technology (1 mentions) Anti hsc70, by Santa Cruz Biotechnology (1 mentions) Anti p21 waf1, by Cell Signaling Technology (1 mentions) Anti cyclin a, by Santa Cruz Biotechnology (1 mentions) Anti p15, by Santa Cruz Biotechnology (1 mentions) Fusion solo, by Vilber (1 mentions) Anti cd36, by GeneTex (1 mentions) Anti actin, by Merck Group (1 mentions)

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TSP-1 (6 citations)

3 protocols using anti tsp 1

Isolating and Characterizing Vascular Endothelial Cells

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Antibodies—anti-VCAM-1 (Biolegend, San Diego, CA, USA), anti-TSP-1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-VE-cadherin and fluorochrome-conjugated anti-CD45 and anti-CD31 (BD Biosciences, San Jose, CA, USA).
Recombinant proteins—mouse IL-17 and TNF-α (R&D Systems, Minneapolis, MN, USA). Type 1A collagenase (Sigma-Aldrich St. Louis, MO, USA) and Type I collagenase (Worthington Biochemical Corp., Lakewood, NJ, USA).
Peptides—TSP-1-derived peptides 4N1K (KRFYVVMWKK, CD47-binding peptide) and CSV (CSVTCG, CD36-binding peptide), and control peptides 4NGG (KRFYGGMWKK) and ANK (ANKHYF), respectively (Bio Basic, Markham, Ontario, Canada).
Culture medium—DMEM containing 10 mM HEPES, 0.1 mM NEAA, 1 mM sodium pyruvate, 100 U/mL penicillin, 100 mg/mL streptomycin, 200 mM L-glutamine (Lonza, Basel, Switzerland), 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA, USA) and endothelial growth supplements (Cell Biologics Inc., Chicago, IL, USA), all purchased from Sigma–Aldrich, unless otherwise stated.

Soriano-Romaní L., Mir F.A., Singh N., Chin I., Hafezi-Moghadam A, & Masli S. (2022). CD47 Binding on Vascular Endothelial Cells Inhibits IL-17-Mediated Leukocyte Adhesion. International Journal of Molecular Sciences, 23(10), 5705.

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Protein Expression Analysis Protocol

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The following antibodies were used: anti-p21Waf1 (Cell Signaling, 2947S), anti-TSP1 (Santa Cruz, sc-12312), anti-p15 (Santa Cruz, sc-612), anti-p-Rb (S780) (BD Pharmingen, 558385), anti-cyclin A (Santa Cruz, sc-271282), rabbit polyclonal anti-Myc (Cell Signaling, 5605S), and anti-hsc-70 (Santa Cruz, sc-7298). Visualization was performed by chemiluminescence with a Bio-Rad Chemi Doc XRS imaging device (Bio-Rad) and Fusion Solo (Vilber).

Guillon J., Petit C., Moreau M., Toutain B., Henry C., Roché H., Bonichon-Lamichhane N., Salmon J.P., Lemonnier J., Campone M., Verrièle V., Lelièvre E., Guette C, & Coqueret O. (2019). Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment. Cell Death & Disease, 10(3), 199.

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Validated Antibody Characterization Protocol

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Chemicals were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise noted. CO at 1000 or 3000 ppm in air was purchased from Air Products and Chemicals, Inc. (Allentown, PA). Antibodies were validated from the purchase company with indicated host specificity and the data sheets of each product were supplied as below: Anti-annexin V-PE (Becton Dickinson/Pharmingen, BD, San Jose, CA cat # 556421), anti-actin (Sigma-Aldrich, St. Louis, Mo, cat# A-2066), anti-AQP4 (Millipore/Sigma, St. Louis, MO, cat#ab3594), anti-CD36 (Genetex, Irvine, CA, cat# GTX20003), anti-Ly6G eFluor450 (Novus Biologicals, Centennial, CO, cat#RB6-8C5), anti-mouse MPO-PE (R & D Systems, Minneapolis, MN, cat# AF3667), anti-mouse CD31 BV510 (BD cat#563089), anti-CD41 PerCP Cy5.5 (BioLegend, San Diego, CA, cat#133918), Anti-GFAP Brilliant Violet 421 (BioLegend, cat# 644719), anti-TMEM119-APC (Abcam, Cambridge, UK, cat#ab225494), anti-NPR AF-790 (Santa Cruz Biotechnology, Dallas, TX cat# sc-390081), anti-MBP PerCP (Novus Biologicals, cat # NBP2–22121PCP), anti-TSP-1 (Santa Cruz, cat#393504 and 393504FITC), anti-p65 subunit of NF-κB (Abcam, cat# ab32536), anti-phosphorylated at serine 536 (Phospho-Ser⁵³⁶) p65 NF-κB) (Cell Signaling, Danvers, MA, cat# 3031).

Ruhela D., Bhopale V.M., Kalakonda S, & Thom S.R. (2021). Astrocyte-derived microparticles initiate a neuroinflammatory cycle due to carbon monoxide poisoning. Brain, Behavior, & Immunity - Health, 18, 100398.

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