For mice, rabbits, and dogs, mitochondrial fractions were prepared in-house using a mitochondrial isolation kit (Cosmo Bio Co., Ltd., Tokyo, Japan) just after the livers were dissected from the animals. Crl:CD1 mice (five 12-week-old males and five 10-week-old females) were purchased from Charles River Laboratories Japan (Kanagawa, Japan). NZW rabbits (five 36-week-old males and six 27- to 32-week-old females) and TOYO beagles (two 7-month-old males and two 7-month-old females) were purchased from Oriental Yeast (Tokyo, Japan). Procedures involving animals and their care conformed to institutional guidelines, which are in compliance with Japanese laws and were approved by the IACUC at our institution.
Crl cd1 mice
Manufactured by Charles River Laboratories
Sourced in Japan
The Crl:CD1 mouse is a widely used outbred mouse strain. It is a general-purpose laboratory mouse model that is commonly used in a variety of research applications.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using crl cd1 mice
1
Mitochondrial Isolation from Animal Livers
2
Murine Model of Vibrio vulnificus Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We purchased 5-week-old male Crl:CD1 mice (Charles River Laboratories Japan, Kanagawa, Japan). The mice were housed in a controlled environment with a 12-h/12-h light/dark cycle and maintained at 23°C. One week later, the mice were utilized in the animal experiments.
Previously, Yamazaki et al. established aV. vulnificus mouse wound infection model (25 (link)). Mice were subcutaneously inoculated with 100 μL of a 10×-diluted bacterial solution adjusted to an OD600 of 1.0 into the right caudal thighs and infected (106 CFU/head) at 23°C after anesthesia using isoflurane. The mice were sacrificed 9 h after the injections. The mouse muscles were dissected and placed in a 1.5-mL tube. Mouse blood samples were collected in 1.5-mL tubes and EDTA dipotassium (EDTA-2K) at 1 mg/mL was added, to prevent blood coagulation.
Neutropenic mice, 6 weeks old, were prepared via a 2-dose intraperitoneal administration of cyclophosphamide monohydrate at 150 mg/kg of body weight at day −4 and 100 mg/kg at day −1 from infection. Neutropenic mice were infected with bacteria using the method used in another experiment. The mice were sacrificed 6 h after the injections. The mouse muscles were dissected and placed in 1.5-mL tubes. Mouse blood samples were collected in 1.5-mL tubes, and EDTA-2K at 1 mg/mL was added to prevent blood coagulation.
Previously, Yamazaki et al. established a
Neutropenic mice, 6 weeks old, were prepared via a 2-dose intraperitoneal administration of cyclophosphamide monohydrate at 150 mg/kg of body weight at day −4 and 100 mg/kg at day −1 from infection. Neutropenic mice were infected with bacteria using the method used in another experiment. The mice were sacrificed 6 h after the injections. The mouse muscles were dissected and placed in 1.5-mL tubes. Mouse blood samples were collected in 1.5-mL tubes, and EDTA-2K at 1 mg/mL was added to prevent blood coagulation.
3
Antimicrobial Effects of D-CATH-2 Peptides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 26 amino acid full d -enantiomer of chicken CATH-2 (RFGRFLRKIRRFRPKVTITIQGSARF-NH2) (d-CATH-2) with a net charge of 9+ and two truncated derivatives (d-C(1 –21), 8+ and d-C(4 –21), 7+) were used in this study. The peptides were synthesized by Fmoc-chemistry at China Peptides (CPC scientific, Sunnyvale, CA, USA) and purified by reverse phase high-performance liquid chromatography to a purity of >95%. Lyophilized peptides were dissolved in endotoxin free water.
S. suis serotype 2 strains P1/7, D282, S735, and OV625 were used in this study. All strains have been previously characterized [30] (link). Bacterial strains were grown overnight at 37 °C from glycerol stocks in Todd-Hewitt broth (THB, Oxoid Ltd., London, UK) before use.
Seven to ten week old Crl:CD-1 mice (both male and female) were purchased from Charles River (Germany). All mice were kept under specific pathogen-free conditions with free access to food and water under the guidelines for animal experimentation as approved by the Dutch central authority for scientific procedures on animals (CCD, License number: AVD108002015175).
S. suis serotype 2 strains P1/7, D282, S735, and OV625 were used in this study. All strains have been previously characterized [30] (link). Bacterial strains were grown overnight at 37 °C from glycerol stocks in Todd-Hewitt broth (THB, Oxoid Ltd., London, UK) before use.
Seven to ten week old Crl:CD-1 mice (both male and female) were purchased from Charles River (Germany). All mice were kept under specific pathogen-free conditions with free access to food and water under the guidelines for animal experimentation as approved by the Dutch central authority for scientific procedures on animals (CCD, License number: AVD108002015175).
4
Immunization of Crl:CD-1 Mice with FlaA and FlaB
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Six- to seven-week-old female Crl:CD-1 mice (Charles River Laboratories, MA) were immunized with FlaA and FlaB proteins by intramuscular administration. Briefly, mice were immunized with 5 µg of proteins or PBS (negative control) on days 0, 14, and 28 (4 (link)). Sera were obtained prior to the first dose and 14 days after the last dose and stored at −20°C.
5
Immunogenic Conjugate Vaccines Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Six-to 7-week-old female Crl:CD-1 mice (Charles River Laboratories, MA) were injected intramuscularly (IM) in the right gastrocnemius at 0, 14 and 28 days with either sterile PBS pH 7.4, the O1:rFlaA conjugate vaccine at 5 μg O1 OPS/dose or the O1:rFlaB conjugate vaccine at 2.5 μg O1 OPS/dose. Serum was obtained by centrifugation of blood collected by retro-orbital bleeding in Z-gel Micro tubes (Sarstedt, Germany) before the first immunization and two weeks after the third vaccination. Serum was stored at -20°C until use. New Zealand White rabbits were immunized under standard protocols at Cocalico Biologicals (PA) by IM injection at 0, 14, 28 and 42 days with quadrivalent conjugate (formulated at 5 μg of each polysaccharide per dose), admixed unconjugated purified OPS and recombinant flagellin molecules (5 μg of each respective OPS and flagellin component), 5 μg rFlaA, or 5 μg rFlaB proteins, with the first dose formulated in complete Freunds adjuvant and the remaining doses formulated in incomplete Freunds adjuvant. Sera were obtained prior to the first dose, 14 days after the third dose and 28 days after the 4th immunization, and stored at -20°C until use.
6
Isolation Rearing and Social Interaction in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experimental subjects were male Crl:CD1 mice (Charles-River Laboratories) obtained from the breeding facility of the Institute of Experimental Medicine (Budapest, Hungary). Mice were maintained at a temperature of 22 ± 1 °C and at a relative humidity of 60 ± 10%, in a light-dark cycle of 12:12 h with lights off at 7AM and on at 7PM. Food and water were available ad libitum, except for the delay discounting training and test. Body weights of mice were regularly measured and did not differ between groups at any time point. Following weaning on postnatal day 21 (PN21), mice were randomly assigned to social (4 mice per cage) or isolation rearing (individually housed) in Plexiglas cages measuring 36,5 × 20,7 × 14 cm. Intruders used in the resident-intruder test were also male Crl:CD1 mice of the same source and were housed socially (4–5 mice per cage), under similar conditions. All experiments were carried out in accordance with the Directive of the European Parliament and the Council from 22 September 2010 (2010/63/EU) and were reviewed and approved by the Animals Welfare Committee of the Institute of Experimental Medicine.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!