Scn400 f brightfield and fluorescence slide scanner

Portions of the liver, ear (skin), lung and colon were fixed in 10% neutral buffered formalin for 1-7 days and transferred to an 80% ethanol solution for storage. Organs were then embedded in a paraffin block and lateral cross-sections were cut and stained with hematoxylin and eosin (H&E) (Histology Services, University of Adelaide). Slides were scanned using a SCN400 F Brightfield and Fluorescence Slide Scanner (Leica Microsystems) at 20X magnification and the CaseViewer software (3DHISTECH Ltd) was used to visualize samples. Liver, skin and lung sections were histologically scored in accordance with the protocol described in Mayer et al.⁸⁶ (link) Liver sections (3-5 separate lobes/mouse) were scored by trained pathologists in a blinded manner.

Sections of liver were fixed in 10% neutral‐buffered formalin for 7 days and then transferred into 80% ethanol for long‐term storage. Liver sections were then cut with a microtome, embedded in paraffin, and stained with hematoxylin and eosin (H&E). Liver embedding in paraffin and H&E staining was carried out by the University of Adelaide Health and Medical School's Histology Department. Images of H&E‐stained slides were acquired by the SAHMRI histology screening service on a SCN400 F Brightfield and Fluorescence Slide Scanner (Leica Microsystems) at 20 × magnification. CaseViewer (3DHISTECH Ltd) was then used to visually score regions of inflammation based on the following criteria set by Mayer et al.¹³ while blinded to treatment groups:

Portal inflammation: 0, no inflammatory infiltrate; 1, low level of inflammatory cell infiltration; 2, moderate level of inflammatory cell infiltration; 3, severe inflammation.

Lobular inflammation: 0, no inflammatory infiltrate; 1, low level of inflammatory cell infiltration; 2, moderate level of inflammatory cell infiltration; 3, severe inflammation (>50% of parenchyma).

Necrosis: 0, none; 1, small necroses; 2, large necrotic areas; 3, bridging necroses.