Rpmi medium 1640 basic 1

Manufactured by Thermo Fisher Scientific

Sourced in United States

RPMI medium 1640 basic (1×) is a widely used cell culture medium formulation that provides essential nutrients and components to support the growth and maintenance of various cell lines in vitro. It is a basal medium that can be supplemented with additional factors as needed for specific cell culture applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Thp1 defnlrp3, by InvivoGen (1 mentions) Thp 1 null, by InvivoGen (1 mentions) Pcmv3, by Sino Biological (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Bca protein concentration assay kit, by Beyotime (1 mentions) Anti p53, by Proteintech (1 mentions) Low melting agarose, by BBI Lifesciences (1 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions) Smm 293 tii medium, by Sino Biological (1 mentions)

Close spelling variants of this product

RPMI 1640 medium (19060 citations) RPMI 1640 (15891 citations) RPMI medium (1417 citations) 1640 medium (927 citations) RPMI Medium 1640 basic (59 citations) 1640 medium (RPMI 1640) (17 citations) RPMI 1640 1× medium (4 citations) RPMI 1640 basic (4 citations) Medium 1640 basic (3 citations) RPMI Medium 1640 basic medium (2 citations)

5 protocols using rpmi medium 1640 basic 1

Culturing Rat Aortic Endothelial Cells and Simvastatin/Glycyrrhizin Preparation

Cited 2 times

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The rat aortic endothelial cells (RAECs) line was purchased from American Type Collection Center (ATCC). RAECs were cultured in RPMI medium 1640 basic (1×) (Gibco, USA), containing 10% of fetal bovine serum (Gibco, USA) and 1% penicillin–streptomycin (Gibco, USA). The cells were incubated in 5% CO₂ at 37°C.
Simvastatin (sigma) was dissolved in 95% ethanol and 0.1 M NaOH, heating at 50°C for 2 h, and neutralizing with HCl to pH 7.2 as described previously [11 (link)]. HMGB1 inhibitor glycyrrhizin (Gly) was purchased from Aladdin was dissolved in 1% ethanol and adjusted to PH7.2 with 1M NaOH [8 (link)].

Lv Z.H., Phuong T.A., Jin S.J., Li X.X, & Xu M. (2017). Protection by simvastatin on hyperglycemia-induced endothelial dysfunction through inhibiting NLRP3 inflammasomes. Oncotarget, 8(53), 91291-91305.

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Generation and Culture of THP-1 Cell Lines

Cited 1 time

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THP-1 cells were from the Cell Resource Center, IBMS, CAMS/PUMC (Beijing, China). The THP-1 cell line with Gsdmd knockout (THP-1^Gsdmd-KO) was generated previously [30 (link)]. Cell culture was performed at 37 °C in a 5% CO₂ humidified incubator. The culture medium was RPMI Medium 1640 basic (1×) (C22400500BT, Gibco) containing 10% (v/v) FBS (10099-141 C, Gibco), 1% penicillin and streptomycin (SV30010, HyClone). THP-1-Null, THP-1-defCasp1 (Casp1 knockdown), and THP-1-defNLRP3 (Nlrp3 knockdown) were purchased from InvivoGen and cultured as instructed by the manufacturer.

Zhao Y, & Sun L. (2022). Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis. Cell Death Discovery, 8, 305.

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Cellular response to DNA damage

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Fetal bovine serum (FBS), streptomycin (CAS: 57-92-1), penicillin (CAS: 61-33-6), and trypsin (1:250) were purchased from Sijiqing Company (Hangzhou, China), while RPMI Medium 1640 Basic (1×) was product of Gibco (Grand Island, NY). Hoechst 33342, Necrostatin-1 (Nec-1), dimethyl sulfoxide (DMSO) and methyl-cyclodextrin (mCD, CAS: 128446-36-6) were the products of SIGMA-ALDRICH (St Louis, MO, USA). Low melting agarose and β-tubulin antibody were products of BBI Life Sciences (Shanghai, China); z-VAD-fmk (CAS: 187389-52-2) and BCA protein concentration assay kit were bought from Beyotime (Shanghai, China). The plasmids pCMV3-TP53-myc and pCMV3 were bought from Sino Biological (Beijing, China). The antibodies used and their sources (in brackets) were: anti-p-H2AX (Ser139) (Bioss, Beijing, China); anti-H2AX and anti-p53 (Proteintech Group, Chicago, USA). The secondary antibodies were from ZS-BIO (Beijing, China).

Cui X., Cao Z., & Wang Z. (2021). DNA damage and necroptosis induced by peroxidase from proso millet in human colorectal cancer cells. Tropical Journal of Pharmaceutical Research, 18(5), 975-983.

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NSCLC Cell Line Cultivation and Authentication

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NSCLC cell lines (A549, PC9, and H226) and normal human pulmonary epithelial cell line (BEAS-2B) were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All cell lines were authenticated by matching the short-tandem repeat (STR) DNA profiles of each cell line to the corresponding standard STR in the database of ATCC and DSMZ. No contamination of mycoplasma was found in these cell lines. A549, PC9, and BEAS-2B cells were cultured in DMEM medium containing 10% fetal bovine serum (FBS, Gibco, NY, USA). H226 Cells were incubated in RPMI-1640 basic (1×) medium (Gibco, NY, USA). All cells were cultured in a humidified atmosphere with 5% CO₂ at 37 °C.

He Q., Yang L., Gao K., Ding P., Chen Q., Xiong J., Yang W., Song Y., Wang L., Wang Y., Ling L., Wu W., Yan J., Zou P., Chen Y, & Zhai R. (2020). FTSJ1 regulates tRNA 2ʹ-O-methyladenosine modification and suppresses the malignancy of NSCLC via inhibiting DRAM1 expression. Cell Death & Disease, 11(5), 348.

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Cell Culture Conditions for Immune Cell Lines

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The MICA⁺ Hmy2.CIR, Daudi, H9, and NKL cell lines were from stocks held in our laboratory. The NKG2D-2B4 and 2B4 cells were licensed for use by the Chengcheng Zhang Laboratory of the University of Texas Southwestern Medical Center (TX, USA). All of the cell lines listed above were cultured in RPMI 1640 basic (1×) medium (Cat#: 11875500BT, Gibco) containing 10% fetal bovine serum (FBS; Cat#: 10099141, Gibco). The medium for the NKL cells also contained 10 ng/mL interleukin-2 (Cat#: 200-02-50UG, PeproTech). All cells were cultured at 37°C in incubators containing 5% CO₂. Additionally, suspension HEK293 cells were cultured in dedicated and serum-free SMM 293-TII medium (Cat#: M293TII, Sino Biological Inc.).

Liu R., Luo Q., Luo W., Wan L., Zhu Q., Yin X., Lu X., Song Z., Wei L., Xiang Z, & Zou Y. (2022). A Soluble NK-CAR Mediates the Specific Cytotoxicity of NK Cells toward the Target CD20+ Lymphoma Cells. Aging and Disease, 13(5), 1576-1588.

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